Pseudomonas aeruginosa significantly increases expression of receptor for advanced glycation endproducts (RAGE) in the septicemia suffering patients

Receptor for Advanced Glycation Endproducts (RAGE) is a cell surface receptor, which recognizes several endogenous and exogenous molecules and subsequently induces expression of several molecules including chemokines. Chemokines are members of the cytokine superfamily and participate in several immu...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	A. Kariminik, F. Hosseini, E. Nasiri
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2021
Materias:	septicemia rage cxcl11 gene expression Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/a4e88fff586e4864ae340cb74a74c02d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a4e88fff586e4864ae340cb74a74c02d
record_format	dspace
spelling	oai:doaj.org-article:a4e88fff586e4864ae340cb74a74c02d2021-11-22T07:09:55ZPseudomonas aeruginosa significantly increases expression of receptor for advanced glycation endproducts (RAGE) in the septicemia suffering patients2220-76192313-739810.15789/2220-7619-PAS-1253https://doaj.org/article/a4e88fff586e4864ae340cb74a74c02d2021-11-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/1253https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398Receptor for Advanced Glycation Endproducts (RAGE) is a cell surface receptor, which recognizes several endogenous and exogenous molecules and subsequently induces expression of several molecules including chemokines. Chemokines are members of the cytokine superfamily and participate in several immune system functions, including cell migration, inflammation, angiogenesis/angiostasis etc. CXC ligand 11 (CXCL11) is an important chemokine which participates in the induction of appropriate immune responses against microbes, including bacteria. The main mechanisms responsible to overcome septicemia are yet to be clarified. Thus, it has been hypothesized that RAGE may participate in induction of CXCL11 in response to the microbial agents. Due to the fact that immune responses play key roles in limitation of infection, it has been proposed that RAGE may inhibit spread of septicemia. Therefore, in this project mRNA levels of RAGE and CXCL11 were explored in the patients suffering from septicemia versus healthy controls. RAGE and CXCL11 expression levels in the 80 subjects, including 40 septicemia patients and 40 healthy controls were explored using Real-Time PCR technique. Accordingly, by using the specific primer against RAGE and CXCL11 in a Rotorgene vehicle the mRNA levels have been determined. The septicemia and the sources of the bacteria in the blood were diagnosed using microbial cultures. The results demonstrated that although mRNA levels for RAGE and CXCL11 did not change in the septicemia patients vs. healthy controls, mRNA levels of RAGE were significantly higher in the patients infected by Pseudomonas aeruginosa compared to those infected by other bacteria, Escherichia coli, Staphylococcus aureus, and Acinetobacter baumannii. RAGE and CXCL11 mRNA levels did not differ among male and female patients. Based on the results it seems that RAGE is a critical receptor against P. aeruginosa during septicemia and more investigations, especially on the RAGE down-stream molecules can clarify its main roles against P. aeruginosa.A. KariminikF. HosseiniE. NasiriSankt-Peterburg : NIIÈM imeni Pasteraarticlesepticemiaragecxcl11gene expressionInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 11, Iss 5, Pp 984-988 (2021)
institution	DOAJ
collection	DOAJ
language	RU
topic	septicemia rage cxcl11 gene expression Infectious and parasitic diseases RC109-216
spellingShingle	septicemia rage cxcl11 gene expression Infectious and parasitic diseases RC109-216 A. Kariminik F. Hosseini E. Nasiri Pseudomonas aeruginosa significantly increases expression of receptor for advanced glycation endproducts (RAGE) in the septicemia suffering patients
description	Receptor for Advanced Glycation Endproducts (RAGE) is a cell surface receptor, which recognizes several endogenous and exogenous molecules and subsequently induces expression of several molecules including chemokines. Chemokines are members of the cytokine superfamily and participate in several immune system functions, including cell migration, inflammation, angiogenesis/angiostasis etc. CXC ligand 11 (CXCL11) is an important chemokine which participates in the induction of appropriate immune responses against microbes, including bacteria. The main mechanisms responsible to overcome septicemia are yet to be clarified. Thus, it has been hypothesized that RAGE may participate in induction of CXCL11 in response to the microbial agents. Due to the fact that immune responses play key roles in limitation of infection, it has been proposed that RAGE may inhibit spread of septicemia. Therefore, in this project mRNA levels of RAGE and CXCL11 were explored in the patients suffering from septicemia versus healthy controls. RAGE and CXCL11 expression levels in the 80 subjects, including 40 septicemia patients and 40 healthy controls were explored using Real-Time PCR technique. Accordingly, by using the specific primer against RAGE and CXCL11 in a Rotorgene vehicle the mRNA levels have been determined. The septicemia and the sources of the bacteria in the blood were diagnosed using microbial cultures. The results demonstrated that although mRNA levels for RAGE and CXCL11 did not change in the septicemia patients vs. healthy controls, mRNA levels of RAGE were significantly higher in the patients infected by Pseudomonas aeruginosa compared to those infected by other bacteria, Escherichia coli, Staphylococcus aureus, and Acinetobacter baumannii. RAGE and CXCL11 mRNA levels did not differ among male and female patients. Based on the results it seems that RAGE is a critical receptor against P. aeruginosa during septicemia and more investigations, especially on the RAGE down-stream molecules can clarify its main roles against P. aeruginosa.
format	article
author	A. Kariminik F. Hosseini E. Nasiri
author_facet	A. Kariminik F. Hosseini E. Nasiri
author_sort	A. Kariminik
title	Pseudomonas aeruginosa significantly increases expression of receptor for advanced glycation endproducts (RAGE) in the septicemia suffering patients
title_short	Pseudomonas aeruginosa significantly increases expression of receptor for advanced glycation endproducts (RAGE) in the septicemia suffering patients
title_full	Pseudomonas aeruginosa significantly increases expression of receptor for advanced glycation endproducts (RAGE) in the septicemia suffering patients
title_fullStr	Pseudomonas aeruginosa significantly increases expression of receptor for advanced glycation endproducts (RAGE) in the septicemia suffering patients
title_full_unstemmed	Pseudomonas aeruginosa significantly increases expression of receptor for advanced glycation endproducts (RAGE) in the septicemia suffering patients
title_sort	pseudomonas aeruginosa significantly increases expression of receptor for advanced glycation endproducts (rage) in the septicemia suffering patients
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2021
url	https://doaj.org/article/a4e88fff586e4864ae340cb74a74c02d
work_keys_str_mv	AT akariminik pseudomonasaeruginosasignificantlyincreasesexpressionofreceptorforadvancedglycationendproductsrageinthesepticemiasufferingpatients AT fhosseini pseudomonasaeruginosasignificantlyincreasesexpressionofreceptorforadvancedglycationendproductsrageinthesepticemiasufferingpatients AT enasiri pseudomonasaeruginosasignificantlyincreasesexpressionofreceptorforadvancedglycationendproductsrageinthesepticemiasufferingpatients
_version_	1718417934100987904

Pseudomonas aeruginosa significantly increases expression of receptor for advanced glycation endproducts (RAGE) in the septicemia suffering patients

Ejemplares similares