The Presence of <i>Treponema</i> spp. in Equine Hoof Canker Biopsies and Skin Samples from Bovine Digital Dermatitis Lesions

Equine hoof canker and bovine digital dermatitis are infectious inflammatory diseases of the hooves with an unknown etiology. However, anaerobic spirochetes of the genus <i>Treponema</i> are considered to be potential etiological agents. The aim of this study was to find a suitable way t...

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Autores principales:	Paulína Marčeková, Marián Mad’ar, Eva Styková, Jana Kačírová, Miriam Sondorová, Pavol Mudroň, Zdeněk Žert
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	equine hoof canker bovine digital dermatitis <i>Treponema</i> PCR Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a4ecb4861eba449ebe496e1337c42683
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spelling	oai:doaj.org-article:a4ecb4861eba449ebe496e1337c426832021-11-25T18:24:05ZThe Presence of <i>Treponema</i> spp. in Equine Hoof Canker Biopsies and Skin Samples from Bovine Digital Dermatitis Lesions10.3390/microorganisms91121902076-2607https://doaj.org/article/a4ecb4861eba449ebe496e1337c426832021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2190https://doaj.org/toc/2076-2607Equine hoof canker and bovine digital dermatitis are infectious inflammatory diseases of the hooves with an unknown etiology. However, anaerobic spirochetes of the genus <i>Treponema</i> are considered to be potential etiological agents. The aim of this study was to find a suitable way to isolate DNA and to detect the presence of treponemal DNA in samples of equine hoof canker and bovine digital dermatitis. DNAzol<sup>®®</sup> Direct and column kits were used to isolate DNA from samples of equine hoof canker and bovine digital dermatitis. The presence of <i>Treponema</i> spp. was detected using PCR and Sanger sequencing. DNAzol<sup>®®</sup> Direct is suitable for isolating DNA from these types of samples. Treponemal DNA was detected in equine hoof samples as well as in bovine digital dermatitis skin samples. In equine hoof biopsies, the most frequently detected was <i>Treponema pedis</i> (8/13). <i>Treponema brennaborense</i> (2/13) and <i>Treponema denticola</i> (2/13) were also found. In the case of bovine digital dermatitis, <i>Treponema medium</i> ssp. <i>bovis</i> was confirmed in 14 of 36 skin samples. <i>Treponema pedis</i> (9/36), <i>Treponema vincentii</i> (1/36), <i>Treponema phagedenis</i> (1/36), and <i>Treponema brennaborense</i> (1/36) were detected as well. DNAzol<sup>®®</sup> Direct was more appropriate for isolation of treponemal DNA because the columns isolation method was more equipment and time-consuming. The presence of several <i>Treponema</i> spp. was determined in the samples. In horses, the most commonly detected species was a <i>T. pedis</i>, while in cattle it was <i>T</i>. <i>medium</i> ssp. <i>bovis</i>.Paulína MarčekováMarián Mad’arEva StykováJana KačírováMiriam SondorováPavol MudroňZdeněk ŽertMDPI AGarticleequine hoof cankerbovine digital dermatitis<i>Treponema</i>PCRBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2190, p 2190 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	equine hoof canker bovine digital dermatitis <i>Treponema</i> PCR Biology (General) QH301-705.5
spellingShingle	equine hoof canker bovine digital dermatitis <i>Treponema</i> PCR Biology (General) QH301-705.5 Paulína Marčeková Marián Mad’ar Eva Styková Jana Kačírová Miriam Sondorová Pavol Mudroň Zdeněk Žert The Presence of <i>Treponema</i> spp. in Equine Hoof Canker Biopsies and Skin Samples from Bovine Digital Dermatitis Lesions
description	Equine hoof canker and bovine digital dermatitis are infectious inflammatory diseases of the hooves with an unknown etiology. However, anaerobic spirochetes of the genus <i>Treponema</i> are considered to be potential etiological agents. The aim of this study was to find a suitable way to isolate DNA and to detect the presence of treponemal DNA in samples of equine hoof canker and bovine digital dermatitis. DNAzol<sup>®®</sup> Direct and column kits were used to isolate DNA from samples of equine hoof canker and bovine digital dermatitis. The presence of <i>Treponema</i> spp. was detected using PCR and Sanger sequencing. DNAzol<sup>®®</sup> Direct is suitable for isolating DNA from these types of samples. Treponemal DNA was detected in equine hoof samples as well as in bovine digital dermatitis skin samples. In equine hoof biopsies, the most frequently detected was <i>Treponema pedis</i> (8/13). <i>Treponema brennaborense</i> (2/13) and <i>Treponema denticola</i> (2/13) were also found. In the case of bovine digital dermatitis, <i>Treponema medium</i> ssp. <i>bovis</i> was confirmed in 14 of 36 skin samples. <i>Treponema pedis</i> (9/36), <i>Treponema vincentii</i> (1/36), <i>Treponema phagedenis</i> (1/36), and <i>Treponema brennaborense</i> (1/36) were detected as well. DNAzol<sup>®®</sup> Direct was more appropriate for isolation of treponemal DNA because the columns isolation method was more equipment and time-consuming. The presence of several <i>Treponema</i> spp. was determined in the samples. In horses, the most commonly detected species was a <i>T. pedis</i>, while in cattle it was <i>T</i>. <i>medium</i> ssp. <i>bovis</i>.
format	article
author	Paulína Marčeková Marián Mad’ar Eva Styková Jana Kačírová Miriam Sondorová Pavol Mudroň Zdeněk Žert
author_facet	Paulína Marčeková Marián Mad’ar Eva Styková Jana Kačírová Miriam Sondorová Pavol Mudroň Zdeněk Žert
author_sort	Paulína Marčeková
title	The Presence of <i>Treponema</i> spp. in Equine Hoof Canker Biopsies and Skin Samples from Bovine Digital Dermatitis Lesions
title_short	The Presence of <i>Treponema</i> spp. in Equine Hoof Canker Biopsies and Skin Samples from Bovine Digital Dermatitis Lesions
title_full	The Presence of <i>Treponema</i> spp. in Equine Hoof Canker Biopsies and Skin Samples from Bovine Digital Dermatitis Lesions
title_fullStr	The Presence of <i>Treponema</i> spp. in Equine Hoof Canker Biopsies and Skin Samples from Bovine Digital Dermatitis Lesions
title_full_unstemmed	The Presence of <i>Treponema</i> spp. in Equine Hoof Canker Biopsies and Skin Samples from Bovine Digital Dermatitis Lesions
title_sort	presence of <i>treponema</i> spp. in equine hoof canker biopsies and skin samples from bovine digital dermatitis lesions
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a4ecb4861eba449ebe496e1337c42683
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The Presence of <i>Treponema</i> spp. in Equine Hoof Canker Biopsies and Skin Samples from Bovine Digital Dermatitis Lesions

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