Effects of dietary glucose oxidase on growth performance and intestinal health of AA broilers challenged by Clostridium perfringens

ABSTRACT: Arbor Acre (AA) broilers were used as the research object to investigate whether glucose oxidase (GOD) has preventive and relieving effects on necrotic enteritis. The experiment was designed as a factorial arrangement of 2 dietary treatments × 2 infection states. Chickens were fed a basal...

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Autores principales:	Yizhu Zhao, Jiahuan Fu, Peng Li, Ningbo Chen, Yanjie Liu, Dan Liu, Yuming Guo
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	broiler necrotic enteritis clostridium perfringens glucose oxidase intestinal health Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/a4f4bfbb2b594b3896d0c8d84a4cfc00
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spelling	oai:doaj.org-article:a4f4bfbb2b594b3896d0c8d84a4cfc002021-11-30T04:13:30ZEffects of dietary glucose oxidase on growth performance and intestinal health of AA broilers challenged by Clostridium perfringens0032-579110.1016/j.psj.2021.101553https://doaj.org/article/a4f4bfbb2b594b3896d0c8d84a4cfc002022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0032579121005757https://doaj.org/toc/0032-5791ABSTRACT: Arbor Acre (AA) broilers were used as the research object to investigate whether glucose oxidase (GOD) has preventive and relieving effects on necrotic enteritis. The experiment was designed as a factorial arrangement of 2 dietary treatments × 2 infection states. Chickens were fed a basal diet or a diet with 150 U/kg GOD, and were challenged with Clostridium perfringens (Cp) or sterile culture medium. In our study, Cp challenge led to intestinal injury, as evidenced by reducing the average daily gain and the average daily feed intake of AA broilers of 14 to 21 d (P < 0.05), increasing the intestinal jejunal lesion score (P < 0.05), reducing the jejunal villi height and villi height/crypt depth (P < 0.05), upregulating the mRNA expression levels jejunal IFN-γ (P < 0.05). The dietary GOD had no significant effects on the growth performance of each growth period, but significantly decreased the ileal pH, increased the height of villi and the ratio of villi height to crypt depth (P < 0.05) and the expression levels of Occludin and Zonula occludens-1 (ZO-1) at d 21. Moreover, dietary GOD and the Cp challenge significantly altered the composition of 21-d ileal microbiota. The Cp challenge decreased the relative abundance of genus Lactobacillus (P = 0.057), and increased the relative abundance of genus Romboutsia (P < 0.05) and genus Veillonella (P = 0.088). The dietary GOD tended to increase the relative abundance of genus Helicobacter (P = 0.066) and decrease the relative abundance of genus Streptococcus (P = 0.071). This study has shown that the supplementation of GOD could promote the integrity of intestinal barrier and the balance of ileal microbiota, but the effects of GOD on NE broilers and its application in actual production need to be further confirmed.Yizhu ZhaoJiahuan FuPeng LiNingbo ChenYanjie LiuDan LiuYuming GuoElsevierarticlebroilernecrotic enteritisclostridium perfringensglucose oxidaseintestinal healthAnimal cultureSF1-1100ENPoultry Science, Vol 101, Iss 1, Pp 101553- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	broiler necrotic enteritis clostridium perfringens glucose oxidase intestinal health Animal culture SF1-1100
spellingShingle	broiler necrotic enteritis clostridium perfringens glucose oxidase intestinal health Animal culture SF1-1100 Yizhu Zhao Jiahuan Fu Peng Li Ningbo Chen Yanjie Liu Dan Liu Yuming Guo Effects of dietary glucose oxidase on growth performance and intestinal health of AA broilers challenged by Clostridium perfringens
description	ABSTRACT: Arbor Acre (AA) broilers were used as the research object to investigate whether glucose oxidase (GOD) has preventive and relieving effects on necrotic enteritis. The experiment was designed as a factorial arrangement of 2 dietary treatments × 2 infection states. Chickens were fed a basal diet or a diet with 150 U/kg GOD, and were challenged with Clostridium perfringens (Cp) or sterile culture medium. In our study, Cp challenge led to intestinal injury, as evidenced by reducing the average daily gain and the average daily feed intake of AA broilers of 14 to 21 d (P < 0.05), increasing the intestinal jejunal lesion score (P < 0.05), reducing the jejunal villi height and villi height/crypt depth (P < 0.05), upregulating the mRNA expression levels jejunal IFN-γ (P < 0.05). The dietary GOD had no significant effects on the growth performance of each growth period, but significantly decreased the ileal pH, increased the height of villi and the ratio of villi height to crypt depth (P < 0.05) and the expression levels of Occludin and Zonula occludens-1 (ZO-1) at d 21. Moreover, dietary GOD and the Cp challenge significantly altered the composition of 21-d ileal microbiota. The Cp challenge decreased the relative abundance of genus Lactobacillus (P = 0.057), and increased the relative abundance of genus Romboutsia (P < 0.05) and genus Veillonella (P = 0.088). The dietary GOD tended to increase the relative abundance of genus Helicobacter (P = 0.066) and decrease the relative abundance of genus Streptococcus (P = 0.071). This study has shown that the supplementation of GOD could promote the integrity of intestinal barrier and the balance of ileal microbiota, but the effects of GOD on NE broilers and its application in actual production need to be further confirmed.
format	article
author	Yizhu Zhao Jiahuan Fu Peng Li Ningbo Chen Yanjie Liu Dan Liu Yuming Guo
author_facet	Yizhu Zhao Jiahuan Fu Peng Li Ningbo Chen Yanjie Liu Dan Liu Yuming Guo
author_sort	Yizhu Zhao
title	Effects of dietary glucose oxidase on growth performance and intestinal health of AA broilers challenged by Clostridium perfringens
title_short	Effects of dietary glucose oxidase on growth performance and intestinal health of AA broilers challenged by Clostridium perfringens
title_full	Effects of dietary glucose oxidase on growth performance and intestinal health of AA broilers challenged by Clostridium perfringens
title_fullStr	Effects of dietary glucose oxidase on growth performance and intestinal health of AA broilers challenged by Clostridium perfringens
title_full_unstemmed	Effects of dietary glucose oxidase on growth performance and intestinal health of AA broilers challenged by Clostridium perfringens
title_sort	effects of dietary glucose oxidase on growth performance and intestinal health of aa broilers challenged by clostridium perfringens
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/a4f4bfbb2b594b3896d0c8d84a4cfc00
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_version_	1718406881939030016

Effects of dietary glucose oxidase on growth performance and intestinal health of AA broilers challenged by Clostridium perfringens

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