Ethanolamine Influences Human Commensal <named-content content-type="genus-species">Escherichia coli</named-content> Growth, Gene Expression, and Competition with Enterohemorrhagic <named-content content-type="genus-species">E. coli</named-content> O157:H7

ABSTRACT A core principle of bacterial pathogenesis is that pathogens preferentially utilize metabolites that commensal bacteria do not in order to sidestep nutritional competition. The metabolite ethanolamine (EA) is well recognized to play a central role in host adaptation for diverse pathogens. E...

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Autores principales:	Carol A. Rowley, Christopher J. Anderson, Melissa M. Kendall
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	ethanolamine metabolism microbiota signaling Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/a4fa6d89eb224f31a8b67fa8a85c1995
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spelling	oai:doaj.org-article:a4fa6d89eb224f31a8b67fa8a85c19952021-11-15T15:58:21ZEthanolamine Influences Human Commensal <named-content content-type="genus-species">Escherichia coli</named-content> Growth, Gene Expression, and Competition with Enterohemorrhagic <named-content content-type="genus-species">E. coli</named-content> O157:H710.1128/mBio.01429-182150-7511https://doaj.org/article/a4fa6d89eb224f31a8b67fa8a85c19952018-11-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01429-18https://doaj.org/toc/2150-7511ABSTRACT A core principle of bacterial pathogenesis is that pathogens preferentially utilize metabolites that commensal bacteria do not in order to sidestep nutritional competition. The metabolite ethanolamine (EA) is well recognized to play a central role in host adaptation for diverse pathogens. EA promotes growth and influences virulence during host infection. Although genes encoding EA utilization have been identified in diverse bacteria (nonpathogenic and pathogenic), a prevailing idea is that commensal bacteria do not utilize EA to enhance growth, and thus, EA is a noncompetitive metabolite for pathogens. Here, we show that EA augments growth of two human commensal strains of Escherichia coli. Significantly, these commensal strains grow more rapidly than, and even outcompete, the pathogen enterohemorrhagic E. coli O157:H7 specifically when EA is provided as the sole nitrogen source. Moreover, EA-dependent signaling is similarly conserved in the human commensal E. coli strain HS and influences expression of adhesins. These findings suggest a more extensive role for EA utilization in bacterial physiology and host-microbiota-pathogen interactions than previously appreciated. IMPORTANCE The microbiota protects the host from invading pathogens by limiting access to nutrients. In turn, bacterial pathogens selectively exploit metabolites not readily used by the microbiota to establish infection. Ethanolamine has been linked to pathogenesis of diverse pathogens by serving as a noncompetitive metabolite that enhances pathogen growth as well as a signal that modulates virulence. Although ethanolamine is abundant in the gastrointestinal tract, the prevailing idea is that commensal bacteria do not utilize EA, and thus, EA utilization has been particularly associated with pathogenesis. Here, we provide evidence that two human commensal Escherichia coli isolates readily utilize ethanolamine to enhance growth, modulate gene expression, and outgrow the pathogen enterohemorrhagic E. coli. These data indicate a more complex role for ethanolamine in host-microbiota-pathogen interactions.Carol A. RowleyChristopher J. AndersonMelissa M. KendallAmerican Society for MicrobiologyarticleethanolaminemetabolismmicrobiotasignalingMicrobiologyQR1-502ENmBio, Vol 9, Iss 5 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	ethanolamine metabolism microbiota signaling Microbiology QR1-502
spellingShingle	ethanolamine metabolism microbiota signaling Microbiology QR1-502 Carol A. Rowley Christopher J. Anderson Melissa M. Kendall Ethanolamine Influences Human Commensal <named-content content-type="genus-species">Escherichia coli</named-content> Growth, Gene Expression, and Competition with Enterohemorrhagic <named-content content-type="genus-species">E. coli</named-content> O157:H7
description	ABSTRACT A core principle of bacterial pathogenesis is that pathogens preferentially utilize metabolites that commensal bacteria do not in order to sidestep nutritional competition. The metabolite ethanolamine (EA) is well recognized to play a central role in host adaptation for diverse pathogens. EA promotes growth and influences virulence during host infection. Although genes encoding EA utilization have been identified in diverse bacteria (nonpathogenic and pathogenic), a prevailing idea is that commensal bacteria do not utilize EA to enhance growth, and thus, EA is a noncompetitive metabolite for pathogens. Here, we show that EA augments growth of two human commensal strains of Escherichia coli. Significantly, these commensal strains grow more rapidly than, and even outcompete, the pathogen enterohemorrhagic E. coli O157:H7 specifically when EA is provided as the sole nitrogen source. Moreover, EA-dependent signaling is similarly conserved in the human commensal E. coli strain HS and influences expression of adhesins. These findings suggest a more extensive role for EA utilization in bacterial physiology and host-microbiota-pathogen interactions than previously appreciated. IMPORTANCE The microbiota protects the host from invading pathogens by limiting access to nutrients. In turn, bacterial pathogens selectively exploit metabolites not readily used by the microbiota to establish infection. Ethanolamine has been linked to pathogenesis of diverse pathogens by serving as a noncompetitive metabolite that enhances pathogen growth as well as a signal that modulates virulence. Although ethanolamine is abundant in the gastrointestinal tract, the prevailing idea is that commensal bacteria do not utilize EA, and thus, EA utilization has been particularly associated with pathogenesis. Here, we provide evidence that two human commensal Escherichia coli isolates readily utilize ethanolamine to enhance growth, modulate gene expression, and outgrow the pathogen enterohemorrhagic E. coli. These data indicate a more complex role for ethanolamine in host-microbiota-pathogen interactions.
format	article
author	Carol A. Rowley Christopher J. Anderson Melissa M. Kendall
author_facet	Carol A. Rowley Christopher J. Anderson Melissa M. Kendall
author_sort	Carol A. Rowley
title	Ethanolamine Influences Human Commensal <named-content content-type="genus-species">Escherichia coli</named-content> Growth, Gene Expression, and Competition with Enterohemorrhagic <named-content content-type="genus-species">E. coli</named-content> O157:H7
title_short	Ethanolamine Influences Human Commensal <named-content content-type="genus-species">Escherichia coli</named-content> Growth, Gene Expression, and Competition with Enterohemorrhagic <named-content content-type="genus-species">E. coli</named-content> O157:H7
title_full	Ethanolamine Influences Human Commensal <named-content content-type="genus-species">Escherichia coli</named-content> Growth, Gene Expression, and Competition with Enterohemorrhagic <named-content content-type="genus-species">E. coli</named-content> O157:H7
title_fullStr	Ethanolamine Influences Human Commensal <named-content content-type="genus-species">Escherichia coli</named-content> Growth, Gene Expression, and Competition with Enterohemorrhagic <named-content content-type="genus-species">E. coli</named-content> O157:H7
title_full_unstemmed	Ethanolamine Influences Human Commensal <named-content content-type="genus-species">Escherichia coli</named-content> Growth, Gene Expression, and Competition with Enterohemorrhagic <named-content content-type="genus-species">E. coli</named-content> O157:H7
title_sort	ethanolamine influences human commensal <named-content content-type="genus-species">escherichia coli</named-content> growth, gene expression, and competition with enterohemorrhagic <named-content content-type="genus-species">e. coli</named-content> o157:h7
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/a4fa6d89eb224f31a8b67fa8a85c1995
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_version_	1718427043736059904

Ethanolamine Influences Human Commensal <named-content content-type="genus-species">Escherichia coli</named-content> Growth, Gene Expression, and Competition with Enterohemorrhagic <named-content content-type="genus-species">E. coli</named-content> O157:H7

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