Profiling of Differentially Expressed MicroRNAs in Saliva of Parkinson's Disease Patients

Objective: This study aims to identify differentially expressed salivary miRNAs and validate the diagnostic potential for idiopathic Parkinson's disease (PD). Also, the disease specificity of candidate miRNAs was evaluated between PD, multiple system atrophy (MSA), and essential tremor (ET).Met...

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Autores principales:	Yanyan Jiang, Jing Chen, Yunchuang Sun, Fan Li, Luhua Wei, Wei Sun, Jianwen Deng, Yun Yuan, Zhaoxia Wang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Parkinson's disease diagnosis saliva biomarker microRNA Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/a501d674c54145d299b895efd5c6a71b
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spelling	oai:doaj.org-article:a501d674c54145d299b895efd5c6a71b2021-12-01T04:14:29ZProfiling of Differentially Expressed MicroRNAs in Saliva of Parkinson's Disease Patients1664-229510.3389/fneur.2021.738530https://doaj.org/article/a501d674c54145d299b895efd5c6a71b2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fneur.2021.738530/fullhttps://doaj.org/toc/1664-2295Objective: This study aims to identify differentially expressed salivary miRNAs and validate the diagnostic potential for idiopathic Parkinson's disease (PD). Also, the disease specificity of candidate miRNAs was evaluated between PD, multiple system atrophy (MSA), and essential tremor (ET).Methods: We collected salivary samples from 50 PD, 20 ET, and 20 MSA patients, as well as 30 healthy controls (HCs). In the discovery phase, salivary miRNA microarray analysis was performed. In-silico analysis was used to investigate the target genes of differentially expressed miRNAs and clustered pathways. In validation phase, RT-qPCR was performed with samples from 30 PD patients and 30 HCs. Subsequently, we investigated candidate miRNAs in all recruited subjects. Receiver operating characteristic curve and Spearman correlation analysis was performed to determine diagnostic usefulness.Results: We identified 43 miRNAs that were differentially expressed between 5 PD patients and 5 HCs by miRNA microarray analysis. Computational analysis revealed the target genes were clustered in the pathways associated with ubiquitin protein ligase activity. The result of RT-qPCR showed that the miR-29a-3p and miR-29c-3p were found to be significantly downregulated (p = 0.004, p = 0.027), whereas the miR-6756-5p was significantly upregulated in 30 PD patients compared with 30 HCs (p = 0.032). The miR-29a-3p expression level in PD patients was significantly lower than ET patients (p = 0.035), but higher than MSA patients (p < 0.0001). The diagnostic efficacy reached a little higher when the combination of miR-29a-3p and miR-29c-3p.Conclusion: The miRNA combination of salivary miR-29a-3p and miR-29c-3p has potential to be a diagnostic biomarker for idiopathic PD.Yanyan JiangJing ChenYunchuang SunFan LiLuhua WeiWei SunJianwen DengJianwen DengYun YuanZhaoxia WangFrontiers Media S.A.articleParkinson's diseasediagnosissalivabiomarkermicroRNANeurology. Diseases of the nervous systemRC346-429ENFrontiers in Neurology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Parkinson's disease diagnosis saliva biomarker microRNA Neurology. Diseases of the nervous system RC346-429
spellingShingle	Parkinson's disease diagnosis saliva biomarker microRNA Neurology. Diseases of the nervous system RC346-429 Yanyan Jiang Jing Chen Yunchuang Sun Fan Li Luhua Wei Wei Sun Jianwen Deng Jianwen Deng Yun Yuan Zhaoxia Wang Profiling of Differentially Expressed MicroRNAs in Saliva of Parkinson's Disease Patients
description	Objective: This study aims to identify differentially expressed salivary miRNAs and validate the diagnostic potential for idiopathic Parkinson's disease (PD). Also, the disease specificity of candidate miRNAs was evaluated between PD, multiple system atrophy (MSA), and essential tremor (ET).Methods: We collected salivary samples from 50 PD, 20 ET, and 20 MSA patients, as well as 30 healthy controls (HCs). In the discovery phase, salivary miRNA microarray analysis was performed. In-silico analysis was used to investigate the target genes of differentially expressed miRNAs and clustered pathways. In validation phase, RT-qPCR was performed with samples from 30 PD patients and 30 HCs. Subsequently, we investigated candidate miRNAs in all recruited subjects. Receiver operating characteristic curve and Spearman correlation analysis was performed to determine diagnostic usefulness.Results: We identified 43 miRNAs that were differentially expressed between 5 PD patients and 5 HCs by miRNA microarray analysis. Computational analysis revealed the target genes were clustered in the pathways associated with ubiquitin protein ligase activity. The result of RT-qPCR showed that the miR-29a-3p and miR-29c-3p were found to be significantly downregulated (p = 0.004, p = 0.027), whereas the miR-6756-5p was significantly upregulated in 30 PD patients compared with 30 HCs (p = 0.032). The miR-29a-3p expression level in PD patients was significantly lower than ET patients (p = 0.035), but higher than MSA patients (p < 0.0001). The diagnostic efficacy reached a little higher when the combination of miR-29a-3p and miR-29c-3p.Conclusion: The miRNA combination of salivary miR-29a-3p and miR-29c-3p has potential to be a diagnostic biomarker for idiopathic PD.
format	article
author	Yanyan Jiang Jing Chen Yunchuang Sun Fan Li Luhua Wei Wei Sun Jianwen Deng Jianwen Deng Yun Yuan Zhaoxia Wang
author_facet	Yanyan Jiang Jing Chen Yunchuang Sun Fan Li Luhua Wei Wei Sun Jianwen Deng Jianwen Deng Yun Yuan Zhaoxia Wang
author_sort	Yanyan Jiang
title	Profiling of Differentially Expressed MicroRNAs in Saliva of Parkinson's Disease Patients
title_short	Profiling of Differentially Expressed MicroRNAs in Saliva of Parkinson's Disease Patients
title_full	Profiling of Differentially Expressed MicroRNAs in Saliva of Parkinson's Disease Patients
title_fullStr	Profiling of Differentially Expressed MicroRNAs in Saliva of Parkinson's Disease Patients
title_full_unstemmed	Profiling of Differentially Expressed MicroRNAs in Saliva of Parkinson's Disease Patients
title_sort	profiling of differentially expressed micrornas in saliva of parkinson's disease patients
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/a501d674c54145d299b895efd5c6a71b
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_version_	1718405920106479616

Profiling of Differentially Expressed MicroRNAs in Saliva of Parkinson's Disease Patients

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