Clinical and Correlated Responses among Steroid Hormones and Oxidant/Antioxidant Biomarkers in Pregnant, Non-Pregnant and Lactating CIDR-Pre-Synchronized Dromedaries (<i>Camelus dromedarius</i>)

Overproduction of free radicals is controlled by antioxidant defense mechanisms. These defense mechanisms are achieved by antioxidant enzymes such as catalase (CAT). The current study aimed to assess the changes in steroid hormones, oxidant/antioxidants biomarkers, lipid profiles/liver functions ind...

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Autores principales:	Ragab H. Mohamed, Arafat Khalphallah, Ken Nakada, Enas Elmeligy, Dalia Hassan, Eman A. Ebissy, Rehab A. Ghandour, Sabry A. Mousa, Ahmed S. A. Hassaneen
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	antioxidants biomarkers camel fertility clinical findings lipid profiles indices steroids hormones oxidative stress Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/a502c8eebb97468495805b8f3433eeab
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id	oai:doaj.org-article:a502c8eebb97468495805b8f3433eeab
record_format	dspace
institution	DOAJ
collection	DOAJ
language	EN
topic	antioxidants biomarkers camel fertility clinical findings lipid profiles indices steroids hormones oxidative stress Veterinary medicine SF600-1100
spellingShingle	antioxidants biomarkers camel fertility clinical findings lipid profiles indices steroids hormones oxidative stress Veterinary medicine SF600-1100 Ragab H. Mohamed Arafat Khalphallah Ken Nakada Enas Elmeligy Dalia Hassan Eman A. Ebissy Rehab A. Ghandour Sabry A. Mousa Ahmed S. A. Hassaneen Clinical and Correlated Responses among Steroid Hormones and Oxidant/Antioxidant Biomarkers in Pregnant, Non-Pregnant and Lactating CIDR-Pre-Synchronized Dromedaries (<i>Camelus dromedarius</i>)
description	Overproduction of free radicals is controlled by antioxidant defense mechanisms. These defense mechanisms are achieved by antioxidant enzymes such as catalase (CAT). The current study aimed to assess the changes in steroid hormones, oxidant/antioxidants biomarkers, lipid profiles/liver functions indices, renal function biomarkers and minerals metabolism in non-pregnant, lactating or pregnant one-humped she-camels (<i>Camelus dromedarius</i>) pre-synchronized with controlled internal drug releasing. The study also focused on the correlational relationships between steroid hormones and the oxidant/antioxidant biomarkers, lipid profiles and liver functions indices, renal functions and mineral metabolism in these she-camels. The study was conducted on apparently healthy dromedary she-camels (<i>n</i> = 60) during breeding season. A sexually active camel-bull was introduced to she-camels pre-synchronized with CIDR. Fifty to sixty days after natural mating, she-camels were examined for pregnancy. She-camels were divided into three main groups according to both pregnancy and lactation as following: pregnant (PREG, <i>n</i> = 38) which was kept as control one, non-pregnant and lactating (LACT, <i>n</i> = 8), and non-pregnant and non-lactating she-camels (NPREG, <i>n</i>= 14). Steroid hormones, i.e., progesterone (P<sub>4</sub>), estradiol (E<sub>2</sub>) and cortisol, oxidant indictors, i.e., malondialdehyde (MDA), antioxidant biomarkers, i.e., superoxide dismutase (SOD), total antioxidant capacity (TAC), CAT and reduced glutathione (GSH), lipid profiles indices, renal functions and related minerals were assessed. The present study confirmed the efficacy of using CIDR for synchronization in she-camels. Significant elevations in serum steroids hormones in PREG compare with LACT and NPREG. The highest concentrations of MDA as lipid peroxidation and oxidative stress indictors and lowest levels of antioxidant biomarkers except for SOD, i.e., TAC, CAT and GSH, were reported in PREG compared with LACT and NPREG. PREG showed the highest liver enzymes activities and lowest total protein values. Remarkable increases in serum concentrations of renal function parameters and phosphorous (P) were observed in PREG when compared with the other two groups. The investigated she-camels revealed significant correlation between steroid hormones and the oxidant biomarkers, antioxidant biomarkers, liver functions, renal functions and minerals metabolism parameters. P<sub>4</sub> showed positive correlations with antioxidant biomarkers, i.e., TAC, CAT and GSH, and serum aspartate aminotransferase (AST) activities, whereas negative correlations were reported between P<sub>4</sub> and renal functions biomarkers, i.e., blood urea nitrogen (BUN), creatinine (Cr) and creatinine kinase (CK), and minerals metabolism parameters, i.e., P and magnesium (Mg), in CIDR pre-synchronized she-camels. In contrast, E<sub>2</sub> and cortisol showed negative correlations with antioxidant biomarkers, i.e., TAC, CAT and GSH, lipid profiles/liver functions indices, i.e., AST, alkaline phosphatase (ALP) and γ-glutamyl transferase (GGT), CK and Mg, however, positive correlations were demonstrated between E<sub>2</sub> and cortisol, and MDA, Cr and P in investigated she-camels. In conclusion, the present study confirmed the efficacy of using CIDR for synchronization in she-camels. The highest MDA levels as indictors for oxidative stress and the lowest antioxidant levels, i.e., TAC, CAT and GSH, except for SOD in pregnant she-camels, were attributable to physiological oxidative stress as excellent compensatory responses observed in the PREG group to face such a physiologic stage. Moreover, lower P levels in non-pregnant she-camels would be contributed to failure of conception or early embryonic death. The investigated she-camels revealed significant correlations between steroid hormones and the oxidant indicators, antioxidant biomarkers, lipid profile indices and renal functions biomarkers that provided better understanding for physiological stress during pregnancy in camels.
format	article
author	Ragab H. Mohamed Arafat Khalphallah Ken Nakada Enas Elmeligy Dalia Hassan Eman A. Ebissy Rehab A. Ghandour Sabry A. Mousa Ahmed S. A. Hassaneen
author_facet	Ragab H. Mohamed Arafat Khalphallah Ken Nakada Enas Elmeligy Dalia Hassan Eman A. Ebissy Rehab A. Ghandour Sabry A. Mousa Ahmed S. A. Hassaneen
author_sort	Ragab H. Mohamed
title	Clinical and Correlated Responses among Steroid Hormones and Oxidant/Antioxidant Biomarkers in Pregnant, Non-Pregnant and Lactating CIDR-Pre-Synchronized Dromedaries (<i>Camelus dromedarius</i>)
title_short	Clinical and Correlated Responses among Steroid Hormones and Oxidant/Antioxidant Biomarkers in Pregnant, Non-Pregnant and Lactating CIDR-Pre-Synchronized Dromedaries (<i>Camelus dromedarius</i>)
title_full	Clinical and Correlated Responses among Steroid Hormones and Oxidant/Antioxidant Biomarkers in Pregnant, Non-Pregnant and Lactating CIDR-Pre-Synchronized Dromedaries (<i>Camelus dromedarius</i>)
title_fullStr	Clinical and Correlated Responses among Steroid Hormones and Oxidant/Antioxidant Biomarkers in Pregnant, Non-Pregnant and Lactating CIDR-Pre-Synchronized Dromedaries (<i>Camelus dromedarius</i>)
title_full_unstemmed	Clinical and Correlated Responses among Steroid Hormones and Oxidant/Antioxidant Biomarkers in Pregnant, Non-Pregnant and Lactating CIDR-Pre-Synchronized Dromedaries (<i>Camelus dromedarius</i>)
title_sort	clinical and correlated responses among steroid hormones and oxidant/antioxidant biomarkers in pregnant, non-pregnant and lactating cidr-pre-synchronized dromedaries (<i>camelus dromedarius</i>)
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a502c8eebb97468495805b8f3433eeab
work_keys_str_mv	AT ragabhmohamed clinicalandcorrelatedresponsesamongsteroidhormonesandoxidantantioxidantbiomarkersinpregnantnonpregnantandlactatingcidrpresynchronizeddromedariesicamelusdromedariusi AT arafatkhalphallah clinicalandcorrelatedresponsesamongsteroidhormonesandoxidantantioxidantbiomarkersinpregnantnonpregnantandlactatingcidrpresynchronizeddromedariesicamelusdromedariusi AT kennakada clinicalandcorrelatedresponsesamongsteroidhormonesandoxidantantioxidantbiomarkersinpregnantnonpregnantandlactatingcidrpresynchronizeddromedariesicamelusdromedariusi AT enaselmeligy clinicalandcorrelatedresponsesamongsteroidhormonesandoxidantantioxidantbiomarkersinpregnantnonpregnantandlactatingcidrpresynchronizeddromedariesicamelusdromedariusi AT daliahassan clinicalandcorrelatedresponsesamongsteroidhormonesandoxidantantioxidantbiomarkersinpregnantnonpregnantandlactatingcidrpresynchronizeddromedariesicamelusdromedariusi AT emanaebissy clinicalandcorrelatedresponsesamongsteroidhormonesandoxidantantioxidantbiomarkersinpregnantnonpregnantandlactatingcidrpresynchronizeddromedariesicamelusdromedariusi AT rehabaghandour clinicalandcorrelatedresponsesamongsteroidhormonesandoxidantantioxidantbiomarkersinpregnantnonpregnantandlactatingcidrpresynchronizeddromedariesicamelusdromedariusi AT sabryamousa clinicalandcorrelatedresponsesamongsteroidhormonesandoxidantantioxidantbiomarkersinpregnantnonpregnantandlactatingcidrpresynchronizeddromedariesicamelusdromedariusi AT ahmedsahassaneen clinicalandcorrelatedresponsesamongsteroidhormonesandoxidantantioxidantbiomarkersinpregnantnonpregnantandlactatingcidrpresynchronizeddromedariesicamelusdromedariusi
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spelling	oai:doaj.org-article:a502c8eebb97468495805b8f3433eeab2021-11-25T19:11:51ZClinical and Correlated Responses among Steroid Hormones and Oxidant/Antioxidant Biomarkers in Pregnant, Non-Pregnant and Lactating CIDR-Pre-Synchronized Dromedaries (<i>Camelus dromedarius</i>)10.3390/vetsci81102472306-7381https://doaj.org/article/a502c8eebb97468495805b8f3433eeab2021-10-01T00:00:00Zhttps://www.mdpi.com/2306-7381/8/11/247https://doaj.org/toc/2306-7381Overproduction of free radicals is controlled by antioxidant defense mechanisms. These defense mechanisms are achieved by antioxidant enzymes such as catalase (CAT). The current study aimed to assess the changes in steroid hormones, oxidant/antioxidants biomarkers, lipid profiles/liver functions indices, renal function biomarkers and minerals metabolism in non-pregnant, lactating or pregnant one-humped she-camels (<i>Camelus dromedarius</i>) pre-synchronized with controlled internal drug releasing. The study also focused on the correlational relationships between steroid hormones and the oxidant/antioxidant biomarkers, lipid profiles and liver functions indices, renal functions and mineral metabolism in these she-camels. The study was conducted on apparently healthy dromedary she-camels (<i>n</i> = 60) during breeding season. A sexually active camel-bull was introduced to she-camels pre-synchronized with CIDR. Fifty to sixty days after natural mating, she-camels were examined for pregnancy. She-camels were divided into three main groups according to both pregnancy and lactation as following: pregnant (PREG, <i>n</i> = 38) which was kept as control one, non-pregnant and lactating (LACT, <i>n</i> = 8), and non-pregnant and non-lactating she-camels (NPREG, <i>n</i>= 14). Steroid hormones, i.e., progesterone (P<sub>4</sub>), estradiol (E<sub>2</sub>) and cortisol, oxidant indictors, i.e., malondialdehyde (MDA), antioxidant biomarkers, i.e., superoxide dismutase (SOD), total antioxidant capacity (TAC), CAT and reduced glutathione (GSH), lipid profiles indices, renal functions and related minerals were assessed. The present study confirmed the efficacy of using CIDR for synchronization in she-camels. Significant elevations in serum steroids hormones in PREG compare with LACT and NPREG. The highest concentrations of MDA as lipid peroxidation and oxidative stress indictors and lowest levels of antioxidant biomarkers except for SOD, i.e., TAC, CAT and GSH, were reported in PREG compared with LACT and NPREG. PREG showed the highest liver enzymes activities and lowest total protein values. Remarkable increases in serum concentrations of renal function parameters and phosphorous (P) were observed in PREG when compared with the other two groups. The investigated she-camels revealed significant correlation between steroid hormones and the oxidant biomarkers, antioxidant biomarkers, liver functions, renal functions and minerals metabolism parameters. P<sub>4</sub> showed positive correlations with antioxidant biomarkers, i.e., TAC, CAT and GSH, and serum aspartate aminotransferase (AST) activities, whereas negative correlations were reported between P<sub>4</sub> and renal functions biomarkers, i.e., blood urea nitrogen (BUN), creatinine (Cr) and creatinine kinase (CK), and minerals metabolism parameters, i.e., P and magnesium (Mg), in CIDR pre-synchronized she-camels. In contrast, E<sub>2</sub> and cortisol showed negative correlations with antioxidant biomarkers, i.e., TAC, CAT and GSH, lipid profiles/liver functions indices, i.e., AST, alkaline phosphatase (ALP) and γ-glutamyl transferase (GGT), CK and Mg, however, positive correlations were demonstrated between E<sub>2</sub> and cortisol, and MDA, Cr and P in investigated she-camels. In conclusion, the present study confirmed the efficacy of using CIDR for synchronization in she-camels. The highest MDA levels as indictors for oxidative stress and the lowest antioxidant levels, i.e., TAC, CAT and GSH, except for SOD in pregnant she-camels, were attributable to physiological oxidative stress as excellent compensatory responses observed in the PREG group to face such a physiologic stage. Moreover, lower P levels in non-pregnant she-camels would be contributed to failure of conception or early embryonic death. The investigated she-camels revealed significant correlations between steroid hormones and the oxidant indicators, antioxidant biomarkers, lipid profile indices and renal functions biomarkers that provided better understanding for physiological stress during pregnancy in camels.Ragab H. MohamedArafat KhalphallahKen NakadaEnas ElmeligyDalia HassanEman A. EbissyRehab A. GhandourSabry A. MousaAhmed S. A. HassaneenMDPI AGarticleantioxidants biomarkerscamel fertilityclinical findingslipid profiles indicessteroids hormonesoxidative stressVeterinary medicineSF600-1100ENVeterinary Sciences, Vol 8, Iss 247, p 247 (2021)

Clinical and Correlated Responses among Steroid Hormones and Oxidant/Antioxidant Biomarkers in Pregnant, Non-Pregnant and Lactating CIDR-Pre-Synchronized Dromedaries (<i>Camelus dromedarius</i>)

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