Lipopeptide biosurfactant pseudofactin II induced apoptosis of melanoma A 375 cells by specific interaction with the plasma membrane.

In the case of melanoma, advances in therapies are slow, which raises the need to evaluate new therapeutic strategies and natural products with potential cancer cell inhibiting effect. Pseudofactin II (PFII), a novel cyclic lipopeptide biosurfactant has been isolated from the Arctic strain of Pseudo...

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Autores principales:	Tomasz Janek, Anna Krasowska, Agata Radwańska, Marcin Łukaszewicz
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a517c7331e204bd79a4a62d3101eb19a
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spelling	oai:doaj.org-article:a517c7331e204bd79a4a62d3101eb19a2021-11-18T07:54:38ZLipopeptide biosurfactant pseudofactin II induced apoptosis of melanoma A 375 cells by specific interaction with the plasma membrane.1932-620310.1371/journal.pone.0057991https://doaj.org/article/a517c7331e204bd79a4a62d3101eb19a2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23483962/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203In the case of melanoma, advances in therapies are slow, which raises the need to evaluate new therapeutic strategies and natural products with potential cancer cell inhibiting effect. Pseudofactin II (PFII), a novel cyclic lipopeptide biosurfactant has been isolated from the Arctic strain of Pseudomonas fluorescens BD5. The aim of this study was to investigate the effect of PFII on A375 melanoma cells compared with the effect of PFII on Normal Human Dermis Fibroblast (NHDF) cells and elucidate the underlying mechanism of PFII cytotoxic activity. Melanoma A375 cells and NHDF cells were exposed to PFII or staurosporine and apoptotic death was assessed by monitoring caspase 3-like activity and DNA fragmentation. From time-dependent monitoring of lactate dehydrogenase (LDH) release, Ca(2+) influx, and a correlation between Critical Micelle Concentration (CMC) we concluded that cell death is the consequence of plasma membrane permeabilisation by micelles. This finding suggests that pro-apoptotic mechanism of PFII is different from previously described cyclic lipopeptides. The mechanism of PFII specificity towards malignant cells remains to be discovered. The results of this study show that PFII could be a new promising anti-melanoma agent.Tomasz JanekAnna KrasowskaAgata RadwańskaMarcin ŁukaszewiczPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e57991 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tomasz Janek Anna Krasowska Agata Radwańska Marcin Łukaszewicz Lipopeptide biosurfactant pseudofactin II induced apoptosis of melanoma A 375 cells by specific interaction with the plasma membrane.
description	In the case of melanoma, advances in therapies are slow, which raises the need to evaluate new therapeutic strategies and natural products with potential cancer cell inhibiting effect. Pseudofactin II (PFII), a novel cyclic lipopeptide biosurfactant has been isolated from the Arctic strain of Pseudomonas fluorescens BD5. The aim of this study was to investigate the effect of PFII on A375 melanoma cells compared with the effect of PFII on Normal Human Dermis Fibroblast (NHDF) cells and elucidate the underlying mechanism of PFII cytotoxic activity. Melanoma A375 cells and NHDF cells were exposed to PFII or staurosporine and apoptotic death was assessed by monitoring caspase 3-like activity and DNA fragmentation. From time-dependent monitoring of lactate dehydrogenase (LDH) release, Ca(2+) influx, and a correlation between Critical Micelle Concentration (CMC) we concluded that cell death is the consequence of plasma membrane permeabilisation by micelles. This finding suggests that pro-apoptotic mechanism of PFII is different from previously described cyclic lipopeptides. The mechanism of PFII specificity towards malignant cells remains to be discovered. The results of this study show that PFII could be a new promising anti-melanoma agent.
format	article
author	Tomasz Janek Anna Krasowska Agata Radwańska Marcin Łukaszewicz
author_facet	Tomasz Janek Anna Krasowska Agata Radwańska Marcin Łukaszewicz
author_sort	Tomasz Janek
title	Lipopeptide biosurfactant pseudofactin II induced apoptosis of melanoma A 375 cells by specific interaction with the plasma membrane.
title_short	Lipopeptide biosurfactant pseudofactin II induced apoptosis of melanoma A 375 cells by specific interaction with the plasma membrane.
title_full	Lipopeptide biosurfactant pseudofactin II induced apoptosis of melanoma A 375 cells by specific interaction with the plasma membrane.
title_fullStr	Lipopeptide biosurfactant pseudofactin II induced apoptosis of melanoma A 375 cells by specific interaction with the plasma membrane.
title_full_unstemmed	Lipopeptide biosurfactant pseudofactin II induced apoptosis of melanoma A 375 cells by specific interaction with the plasma membrane.
title_sort	lipopeptide biosurfactant pseudofactin ii induced apoptosis of melanoma a 375 cells by specific interaction with the plasma membrane.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/a517c7331e204bd79a4a62d3101eb19a
work_keys_str_mv	AT tomaszjanek lipopeptidebiosurfactantpseudofactiniiinducedapoptosisofmelanomaa375cellsbyspecificinteractionwiththeplasmamembrane AT annakrasowska lipopeptidebiosurfactantpseudofactiniiinducedapoptosisofmelanomaa375cellsbyspecificinteractionwiththeplasmamembrane AT agataradwanska lipopeptidebiosurfactantpseudofactiniiinducedapoptosisofmelanomaa375cellsbyspecificinteractionwiththeplasmamembrane AT marcinłukaszewicz lipopeptidebiosurfactantpseudofactiniiinducedapoptosisofmelanomaa375cellsbyspecificinteractionwiththeplasmamembrane
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Lipopeptide biosurfactant pseudofactin II induced apoptosis of melanoma A 375 cells by specific interaction with the plasma membrane.

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