Polar localization of virulence-related Esx-1 secretion in mycobacteria.

The Esx-1 (type VII) secretion system is critical for virulence of both Mycobacterium tuberculosis and Mycobacterium marinum, and is highly conserved between the two species. Despite its importance, there has been no direct visualization of Esx-1 secretion until now. In M. marinum, we show that secr...

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Autores principales:	Fredric Carlsson, Shilpa A Joshi, Linda Rangell, Eric J Brown
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a518afe6c7aa45c8add68e3a943c7497
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spelling	oai:doaj.org-article:a518afe6c7aa45c8add68e3a943c74972021-11-25T05:47:19ZPolar localization of virulence-related Esx-1 secretion in mycobacteria.1553-73661553-737410.1371/journal.ppat.1000285https://doaj.org/article/a518afe6c7aa45c8add68e3a943c74972009-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19180234/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374The Esx-1 (type VII) secretion system is critical for virulence of both Mycobacterium tuberculosis and Mycobacterium marinum, and is highly conserved between the two species. Despite its importance, there has been no direct visualization of Esx-1 secretion until now. In M. marinum, we show that secretion of Mh3864, a novel Esx-1 substrate that remains partially cell wall-associated after translocation, occurred in polar regions, indicating that Esx-1 secretion takes place in these regions. Analysis of Esx-1 secretion in infected host cells suggested that Esx-1 activity is similarly localized in vivo. A core component of the Esx-1 apparatus, Mh3870, also localized to bacterial poles, showing a preference for new poles with active cell wall peptidoglycan (PGN) synthesis. This work demonstrates that the Esx-1 secretion machine localizes to, and is active at, the bacterial poles. Thus, virulence-related protein secretion is localized in mycobacteria, suggesting new potential therapeutic targets, which are urgently needed.Fredric CarlssonShilpa A JoshiLinda RangellEric J BrownPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 5, Iss 1, p e1000285 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Fredric Carlsson Shilpa A Joshi Linda Rangell Eric J Brown Polar localization of virulence-related Esx-1 secretion in mycobacteria.
description	The Esx-1 (type VII) secretion system is critical for virulence of both Mycobacterium tuberculosis and Mycobacterium marinum, and is highly conserved between the two species. Despite its importance, there has been no direct visualization of Esx-1 secretion until now. In M. marinum, we show that secretion of Mh3864, a novel Esx-1 substrate that remains partially cell wall-associated after translocation, occurred in polar regions, indicating that Esx-1 secretion takes place in these regions. Analysis of Esx-1 secretion in infected host cells suggested that Esx-1 activity is similarly localized in vivo. A core component of the Esx-1 apparatus, Mh3870, also localized to bacterial poles, showing a preference for new poles with active cell wall peptidoglycan (PGN) synthesis. This work demonstrates that the Esx-1 secretion machine localizes to, and is active at, the bacterial poles. Thus, virulence-related protein secretion is localized in mycobacteria, suggesting new potential therapeutic targets, which are urgently needed.
format	article
author	Fredric Carlsson Shilpa A Joshi Linda Rangell Eric J Brown
author_facet	Fredric Carlsson Shilpa A Joshi Linda Rangell Eric J Brown
author_sort	Fredric Carlsson
title	Polar localization of virulence-related Esx-1 secretion in mycobacteria.
title_short	Polar localization of virulence-related Esx-1 secretion in mycobacteria.
title_full	Polar localization of virulence-related Esx-1 secretion in mycobacteria.
title_fullStr	Polar localization of virulence-related Esx-1 secretion in mycobacteria.
title_full_unstemmed	Polar localization of virulence-related Esx-1 secretion in mycobacteria.
title_sort	polar localization of virulence-related esx-1 secretion in mycobacteria.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/a518afe6c7aa45c8add68e3a943c7497
work_keys_str_mv	AT fredriccarlsson polarlocalizationofvirulencerelatedesx1secretioninmycobacteria AT shilpaajoshi polarlocalizationofvirulencerelatedesx1secretioninmycobacteria AT lindarangell polarlocalizationofvirulencerelatedesx1secretioninmycobacteria AT ericjbrown polarlocalizationofvirulencerelatedesx1secretioninmycobacteria
_version_	1718414474660020224

Polar localization of virulence-related Esx-1 secretion in mycobacteria.

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