The PINK1—Parkin mitophagy signalling pathway is not functional in peripheral blood mononuclear cells

Mutations in the PINK1 and PRKN genes are the most common cause of early-onset familial Parkinson disease. These genes code for the PINK1 and Parkin proteins, respectively, which are involved in the degradation of dysfunctional mitochondria through mitophagy. An early step in PINK1 –Parkin mediated...

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Autores principales:	Aaron V. Bradshaw, Philip Campbell, Anthony H. V. Schapira, Huw R. Morris, Jan-Willem Taanman
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a5194ae77b30451d96bafab773aefae9
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spelling	oai:doaj.org-article:a5194ae77b30451d96bafab773aefae92021-11-18T08:14:33ZThe PINK1—Parkin mitophagy signalling pathway is not functional in peripheral blood mononuclear cells1932-6203https://doaj.org/article/a5194ae77b30451d96bafab773aefae92021-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8584748/?tool=EBIhttps://doaj.org/toc/1932-6203Mutations in the PINK1 and PRKN genes are the most common cause of early-onset familial Parkinson disease. These genes code for the PINK1 and Parkin proteins, respectively, which are involved in the degradation of dysfunctional mitochondria through mitophagy. An early step in PINK1 –Parkin mediated mitophagy is the ubiquitination of the mitofusin proteins MFN1 and -2. The ubiquitination of MFN1 and -2 in patient samples may therefore serve as a biomarker to determine the functional effects of PINK1 and PRKN mutations, and to screen idiopathic patients for potential mitophagy defects. We aimed to characterise the expression of the PINK1 –Parkin mitophagy machinery in peripheral blood mononuclear cells (PBMCs) and assess if these cells could serve as a platform to evaluate mitophagy via analysis of MFN1 and -2 ubiquitination. Mitophagy was induced through mitochondrial depolarisation by treatment with the protonophore CCCP and ubiquitinated MFN proteins were analysed by western blotting. In addition, PINK1 and PRKN mRNA and protein expression levels were characterised with reverse transcriptase quantitative PCR and western blotting, respectively. Whilst CCCP treatment led to MFN ubiquitination in primary fibroblasts, SH-SY5Y neuroblastoma cells and Jurkat leukaemic cells, treatment of PBMCs did not induce ubiquitination of MFN. PRKN mRNA and protein was readily detectable in PBMCs at comparable levels to those observed in Jurkat and fibroblast cells. In contrast, PINK1 protein was undetectable and PINK1 mRNA levels were remarkably low in control PBMCs. Our findings suggest that the PINK1 –Parkin mitophagy signalling pathway is not functional in PBMCs. Therefore, PBMCs are not a suitable biosample for analysis of mitophagy function in Parkinson disease patients.Aaron V. BradshawPhilip CampbellAnthony H. V. SchapiraHuw R. MorrisJan-Willem TaanmanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aaron V. Bradshaw Philip Campbell Anthony H. V. Schapira Huw R. Morris Jan-Willem Taanman The PINK1—Parkin mitophagy signalling pathway is not functional in peripheral blood mononuclear cells
description	Mutations in the PINK1 and PRKN genes are the most common cause of early-onset familial Parkinson disease. These genes code for the PINK1 and Parkin proteins, respectively, which are involved in the degradation of dysfunctional mitochondria through mitophagy. An early step in PINK1 –Parkin mediated mitophagy is the ubiquitination of the mitofusin proteins MFN1 and -2. The ubiquitination of MFN1 and -2 in patient samples may therefore serve as a biomarker to determine the functional effects of PINK1 and PRKN mutations, and to screen idiopathic patients for potential mitophagy defects. We aimed to characterise the expression of the PINK1 –Parkin mitophagy machinery in peripheral blood mononuclear cells (PBMCs) and assess if these cells could serve as a platform to evaluate mitophagy via analysis of MFN1 and -2 ubiquitination. Mitophagy was induced through mitochondrial depolarisation by treatment with the protonophore CCCP and ubiquitinated MFN proteins were analysed by western blotting. In addition, PINK1 and PRKN mRNA and protein expression levels were characterised with reverse transcriptase quantitative PCR and western blotting, respectively. Whilst CCCP treatment led to MFN ubiquitination in primary fibroblasts, SH-SY5Y neuroblastoma cells and Jurkat leukaemic cells, treatment of PBMCs did not induce ubiquitination of MFN. PRKN mRNA and protein was readily detectable in PBMCs at comparable levels to those observed in Jurkat and fibroblast cells. In contrast, PINK1 protein was undetectable and PINK1 mRNA levels were remarkably low in control PBMCs. Our findings suggest that the PINK1 –Parkin mitophagy signalling pathway is not functional in PBMCs. Therefore, PBMCs are not a suitable biosample for analysis of mitophagy function in Parkinson disease patients.
format	article
author	Aaron V. Bradshaw Philip Campbell Anthony H. V. Schapira Huw R. Morris Jan-Willem Taanman
author_facet	Aaron V. Bradshaw Philip Campbell Anthony H. V. Schapira Huw R. Morris Jan-Willem Taanman
author_sort	Aaron V. Bradshaw
title	The PINK1—Parkin mitophagy signalling pathway is not functional in peripheral blood mononuclear cells
title_short	The PINK1—Parkin mitophagy signalling pathway is not functional in peripheral blood mononuclear cells
title_full	The PINK1—Parkin mitophagy signalling pathway is not functional in peripheral blood mononuclear cells
title_fullStr	The PINK1—Parkin mitophagy signalling pathway is not functional in peripheral blood mononuclear cells
title_full_unstemmed	The PINK1—Parkin mitophagy signalling pathway is not functional in peripheral blood mononuclear cells
title_sort	pink1—parkin mitophagy signalling pathway is not functional in peripheral blood mononuclear cells
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/a5194ae77b30451d96bafab773aefae9
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The PINK1—Parkin mitophagy signalling pathway is not functional in peripheral blood mononuclear cells

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