Levels of lycopene β-cyclase 1 modulate carotenoid gene expression and accumulation in Daucus carota.

Plant carotenoids are synthesized and accumulated in plastids through a highly regulated pathway. Lycopene β-cyclase (LCYB) is a key enzyme involved directly in the synthesis of α-carotene and β-carotene through the cyclization of lycopene. Carotenoids are produced in both carrot (Daucus carota) lea...

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Autores principales:	Juan Camilo Moreno, Lorena Pizarro, Paulina Fuentes, Michael Handford, Victor Cifuentes, Claudia Stange
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a51a0495e9b64f2cbbc622b56dc1f723
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spelling	oai:doaj.org-article:a51a0495e9b64f2cbbc622b56dc1f7232021-11-18T07:51:26ZLevels of lycopene β-cyclase 1 modulate carotenoid gene expression and accumulation in Daucus carota.1932-620310.1371/journal.pone.0058144https://doaj.org/article/a51a0495e9b64f2cbbc622b56dc1f7232013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23555569/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Plant carotenoids are synthesized and accumulated in plastids through a highly regulated pathway. Lycopene β-cyclase (LCYB) is a key enzyme involved directly in the synthesis of α-carotene and β-carotene through the cyclization of lycopene. Carotenoids are produced in both carrot (Daucus carota) leaves and reserve roots, and high amounts of α-carotene and β-carotene accumulate in the latter. In some plant models, the presence of different isoforms of carotenogenic genes is associated with an organ-specific function. D. carota harbors two Lcyb genes, of which DcLcyb1 is expressed in leaves and storage roots during carrot development, correlating with an increase in carotenoid levels. In this work, we show that DcLCYB1 is localized in the plastid and that it is a functional enzyme, as demonstrated by heterologous complementation in Escherichia coli and over expression and post transcriptional gene silencing in carrot. Transgenic plants with higher or reduced levels of DcLcyb1 had incremented or reduced levels of chlorophyll, total carotenoids and β-carotene in leaves and in the storage roots, respectively. In addition, changes in the expression of DcLcyb1 are accompanied by a modulation in the expression of key endogenous carotenogenic genes. Our results indicate that DcLcyb1 does not possess an organ specific function and modulate carotenoid gene expression and accumulation in carrot leaves and storage roots.Juan Camilo MorenoLorena PizarroPaulina FuentesMichael HandfordVictor CifuentesClaudia StangePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e58144 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Juan Camilo Moreno Lorena Pizarro Paulina Fuentes Michael Handford Victor Cifuentes Claudia Stange Levels of lycopene β-cyclase 1 modulate carotenoid gene expression and accumulation in Daucus carota.
description	Plant carotenoids are synthesized and accumulated in plastids through a highly regulated pathway. Lycopene β-cyclase (LCYB) is a key enzyme involved directly in the synthesis of α-carotene and β-carotene through the cyclization of lycopene. Carotenoids are produced in both carrot (Daucus carota) leaves and reserve roots, and high amounts of α-carotene and β-carotene accumulate in the latter. In some plant models, the presence of different isoforms of carotenogenic genes is associated with an organ-specific function. D. carota harbors two Lcyb genes, of which DcLcyb1 is expressed in leaves and storage roots during carrot development, correlating with an increase in carotenoid levels. In this work, we show that DcLCYB1 is localized in the plastid and that it is a functional enzyme, as demonstrated by heterologous complementation in Escherichia coli and over expression and post transcriptional gene silencing in carrot. Transgenic plants with higher or reduced levels of DcLcyb1 had incremented or reduced levels of chlorophyll, total carotenoids and β-carotene in leaves and in the storage roots, respectively. In addition, changes in the expression of DcLcyb1 are accompanied by a modulation in the expression of key endogenous carotenogenic genes. Our results indicate that DcLcyb1 does not possess an organ specific function and modulate carotenoid gene expression and accumulation in carrot leaves and storage roots.
format	article
author	Juan Camilo Moreno Lorena Pizarro Paulina Fuentes Michael Handford Victor Cifuentes Claudia Stange
author_facet	Juan Camilo Moreno Lorena Pizarro Paulina Fuentes Michael Handford Victor Cifuentes Claudia Stange
author_sort	Juan Camilo Moreno
title	Levels of lycopene β-cyclase 1 modulate carotenoid gene expression and accumulation in Daucus carota.
title_short	Levels of lycopene β-cyclase 1 modulate carotenoid gene expression and accumulation in Daucus carota.
title_full	Levels of lycopene β-cyclase 1 modulate carotenoid gene expression and accumulation in Daucus carota.
title_fullStr	Levels of lycopene β-cyclase 1 modulate carotenoid gene expression and accumulation in Daucus carota.
title_full_unstemmed	Levels of lycopene β-cyclase 1 modulate carotenoid gene expression and accumulation in Daucus carota.
title_sort	levels of lycopene β-cyclase 1 modulate carotenoid gene expression and accumulation in daucus carota.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/a51a0495e9b64f2cbbc622b56dc1f723
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Levels of lycopene β-cyclase 1 modulate carotenoid gene expression and accumulation in Daucus carota.

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