Polymer-encased nanodiscs with improved buffer compatibility

Abstract Styrene-maleic acid copolymers allow for solubilization and reconstitution of membrane proteins into nanodiscs. These polymer-encased nanodiscs are promising platforms for studies of membrane proteins in a near-physiologic environment without the use of detergents. However, current styrene-...

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Autores principales:	Mariana C. Fiori, Yunjiang Jiang, Guillermo A. Altenberg, Hongjun Liang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a51c6437cf824dffbda713530a5202a9
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spelling	oai:doaj.org-article:a51c6437cf824dffbda713530a5202a92021-12-02T15:05:19ZPolymer-encased nanodiscs with improved buffer compatibility10.1038/s41598-017-07110-12045-2322https://doaj.org/article/a51c6437cf824dffbda713530a5202a92017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07110-1https://doaj.org/toc/2045-2322Abstract Styrene-maleic acid copolymers allow for solubilization and reconstitution of membrane proteins into nanodiscs. These polymer-encased nanodiscs are promising platforms for studies of membrane proteins in a near-physiologic environment without the use of detergents. However, current styrene-maleic acid copolymers display severe limitations in terms of buffer compatibility and ensued flexibility for various applications. Here, we present a new family of styrene-maleic acid copolymers that do not aggregate at low pH or in the presence of polyvalent cations, and can be used to solubilize membrane proteins and produce nanodiscs of controlled sizes.Mariana C. FioriYunjiang JiangGuillermo A. AltenbergHongjun LiangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mariana C. Fiori Yunjiang Jiang Guillermo A. Altenberg Hongjun Liang Polymer-encased nanodiscs with improved buffer compatibility
description	Abstract Styrene-maleic acid copolymers allow for solubilization and reconstitution of membrane proteins into nanodiscs. These polymer-encased nanodiscs are promising platforms for studies of membrane proteins in a near-physiologic environment without the use of detergents. However, current styrene-maleic acid copolymers display severe limitations in terms of buffer compatibility and ensued flexibility for various applications. Here, we present a new family of styrene-maleic acid copolymers that do not aggregate at low pH or in the presence of polyvalent cations, and can be used to solubilize membrane proteins and produce nanodiscs of controlled sizes.
format	article
author	Mariana C. Fiori Yunjiang Jiang Guillermo A. Altenberg Hongjun Liang
author_facet	Mariana C. Fiori Yunjiang Jiang Guillermo A. Altenberg Hongjun Liang
author_sort	Mariana C. Fiori
title	Polymer-encased nanodiscs with improved buffer compatibility
title_short	Polymer-encased nanodiscs with improved buffer compatibility
title_full	Polymer-encased nanodiscs with improved buffer compatibility
title_fullStr	Polymer-encased nanodiscs with improved buffer compatibility
title_full_unstemmed	Polymer-encased nanodiscs with improved buffer compatibility
title_sort	polymer-encased nanodiscs with improved buffer compatibility
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a51c6437cf824dffbda713530a5202a9
work_keys_str_mv	AT marianacfiori polymerencasednanodiscswithimprovedbuffercompatibility AT yunjiangjiang polymerencasednanodiscswithimprovedbuffercompatibility AT guillermoaaltenberg polymerencasednanodiscswithimprovedbuffercompatibility AT hongjunliang polymerencasednanodiscswithimprovedbuffercompatibility
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Polymer-encased nanodiscs with improved buffer compatibility

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