Validation of the three-chamber strategy for studying mate choice in medaka

The three-chamber experiment, in which one test animal can choose between two animals placed in physically inaccessible compartments, is a widely adopted strategy for studying sexual preference in animals. Medaka, a small freshwater teleost, is an emerging model for dissecting the neurological/physi...

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Autores principales:	Ena Kaneko, Hinako Sato, Shoji Fukamachi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a52430bd81bb4dc3adcef1403be1ee24
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spelling	oai:doaj.org-article:a52430bd81bb4dc3adcef1403be1ee242021-11-25T06:10:56ZValidation of the three-chamber strategy for studying mate choice in medaka1932-6203https://doaj.org/article/a52430bd81bb4dc3adcef1403be1ee242021-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8592428/?tool=EBIhttps://doaj.org/toc/1932-6203The three-chamber experiment, in which one test animal can choose between two animals placed in physically inaccessible compartments, is a widely adopted strategy for studying sexual preference in animals. Medaka, a small freshwater teleost, is an emerging model for dissecting the neurological/physiological mechanisms underlying mate choice for which intriguing findings have been accumulating. The three-chamber strategy has rarely been adopted in this species; therefore, here we investigated its validity using medaka colour variants that mate assortatively. First, a total of 551 movies, in which a test male and two choice females interacted for 30 min under a free-swimming condition, were manually analysed. The sexual preference of the males, calculated as a courtship ratio, was highly consistent between human observers (r > 0.96), supporting the objectivity of this manual-counting strategy. Second, we tested two types of three-chamber apparatuses, in which choice fish were presented in either a face-to-face or side-by-side location. Test fish (regardless of sex) spent most of the time associating with choice fish in the compartments. However, their sexual preference, calculated as an association ratio, was poorly reproduced when the locations of the choice fish were swapped. Third, the sexual preferences of males quantified using the manual-counting and either of the three-chamber strategies did not correlate (r = 0.147 or 0.297). Hence, we concluded that, even for individuals of a species like medaka, which spawn every day, sexual preference could not be reliably evaluated using the three-chamber strategy. Optimization of the protocol may solve this problem; however, the explanation for the observation that animals that are ready for spawning persist with never-accessible mating partners must be reconsidered.Ena KanekoHinako SatoShoji FukamachiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ena Kaneko Hinako Sato Shoji Fukamachi Validation of the three-chamber strategy for studying mate choice in medaka
description	The three-chamber experiment, in which one test animal can choose between two animals placed in physically inaccessible compartments, is a widely adopted strategy for studying sexual preference in animals. Medaka, a small freshwater teleost, is an emerging model for dissecting the neurological/physiological mechanisms underlying mate choice for which intriguing findings have been accumulating. The three-chamber strategy has rarely been adopted in this species; therefore, here we investigated its validity using medaka colour variants that mate assortatively. First, a total of 551 movies, in which a test male and two choice females interacted for 30 min under a free-swimming condition, were manually analysed. The sexual preference of the males, calculated as a courtship ratio, was highly consistent between human observers (r > 0.96), supporting the objectivity of this manual-counting strategy. Second, we tested two types of three-chamber apparatuses, in which choice fish were presented in either a face-to-face or side-by-side location. Test fish (regardless of sex) spent most of the time associating with choice fish in the compartments. However, their sexual preference, calculated as an association ratio, was poorly reproduced when the locations of the choice fish were swapped. Third, the sexual preferences of males quantified using the manual-counting and either of the three-chamber strategies did not correlate (r = 0.147 or 0.297). Hence, we concluded that, even for individuals of a species like medaka, which spawn every day, sexual preference could not be reliably evaluated using the three-chamber strategy. Optimization of the protocol may solve this problem; however, the explanation for the observation that animals that are ready for spawning persist with never-accessible mating partners must be reconsidered.
format	article
author	Ena Kaneko Hinako Sato Shoji Fukamachi
author_facet	Ena Kaneko Hinako Sato Shoji Fukamachi
author_sort	Ena Kaneko
title	Validation of the three-chamber strategy for studying mate choice in medaka
title_short	Validation of the three-chamber strategy for studying mate choice in medaka
title_full	Validation of the three-chamber strategy for studying mate choice in medaka
title_fullStr	Validation of the three-chamber strategy for studying mate choice in medaka
title_full_unstemmed	Validation of the three-chamber strategy for studying mate choice in medaka
title_sort	validation of the three-chamber strategy for studying mate choice in medaka
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/a52430bd81bb4dc3adcef1403be1ee24
work_keys_str_mv	AT enakaneko validationofthethreechamberstrategyforstudyingmatechoiceinmedaka AT hinakosato validationofthethreechamberstrategyforstudyingmatechoiceinmedaka AT shojifukamachi validationofthethreechamberstrategyforstudyingmatechoiceinmedaka
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Validation of the three-chamber strategy for studying mate choice in medaka

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