Stimulation triggers endogenous activity patterns in cultured cortical networks

Abstract Cultures of dissociated cortical neurons represent a powerful trade-off between more realistic experimental models and abstract modeling approaches, allowing to investigate mechanisms of synchronized activity generation. These networks spontaneously alternate periods of high activity (i.e....

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Autores principales:	Valentina Pasquale, Sergio Martinoia, Michela Chiappalone
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a52c794d3f9a4807a310597e809bc395
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spelling	oai:doaj.org-article:a52c794d3f9a4807a310597e809bc3952021-12-02T15:06:22ZStimulation triggers endogenous activity patterns in cultured cortical networks10.1038/s41598-017-08369-02045-2322https://doaj.org/article/a52c794d3f9a4807a310597e809bc3952017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08369-0https://doaj.org/toc/2045-2322Abstract Cultures of dissociated cortical neurons represent a powerful trade-off between more realistic experimental models and abstract modeling approaches, allowing to investigate mechanisms of synchronized activity generation. These networks spontaneously alternate periods of high activity (i.e. network bursts) with periods of quiescence in a dynamic state which recalls the fluctuation of in vivo UP and DOWN states. Network bursts can also be elicited by external stimulation and their spatial propagation patterns tracked by means of multi-channel micro-electrode arrays. In this study, we used rat cortical cultures coupled to micro-electrode arrays to investigate the similarity between spontaneous and evoked activity patterns. We performed experiments by applying electrical stimulation to different network locations and demonstrated that the rank orders of electrodes during evoked and spontaneous events are remarkably similar independently from the stimulation source. We linked this result to the capability of stimulation to evoke firing in highly active and “leader” sites of the network, reliably and rapidly recruited within both spontaneous and evoked bursts. Our study provides the first evidence that spontaneous and evoked activity similarity is reliably observed also in dissociated cortical networks.Valentina PasqualeSergio MartinoiaMichela ChiappaloneNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-16 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Valentina Pasquale Sergio Martinoia Michela Chiappalone Stimulation triggers endogenous activity patterns in cultured cortical networks
description	Abstract Cultures of dissociated cortical neurons represent a powerful trade-off between more realistic experimental models and abstract modeling approaches, allowing to investigate mechanisms of synchronized activity generation. These networks spontaneously alternate periods of high activity (i.e. network bursts) with periods of quiescence in a dynamic state which recalls the fluctuation of in vivo UP and DOWN states. Network bursts can also be elicited by external stimulation and their spatial propagation patterns tracked by means of multi-channel micro-electrode arrays. In this study, we used rat cortical cultures coupled to micro-electrode arrays to investigate the similarity between spontaneous and evoked activity patterns. We performed experiments by applying electrical stimulation to different network locations and demonstrated that the rank orders of electrodes during evoked and spontaneous events are remarkably similar independently from the stimulation source. We linked this result to the capability of stimulation to evoke firing in highly active and “leader” sites of the network, reliably and rapidly recruited within both spontaneous and evoked bursts. Our study provides the first evidence that spontaneous and evoked activity similarity is reliably observed also in dissociated cortical networks.
format	article
author	Valentina Pasquale Sergio Martinoia Michela Chiappalone
author_facet	Valentina Pasquale Sergio Martinoia Michela Chiappalone
author_sort	Valentina Pasquale
title	Stimulation triggers endogenous activity patterns in cultured cortical networks
title_short	Stimulation triggers endogenous activity patterns in cultured cortical networks
title_full	Stimulation triggers endogenous activity patterns in cultured cortical networks
title_fullStr	Stimulation triggers endogenous activity patterns in cultured cortical networks
title_full_unstemmed	Stimulation triggers endogenous activity patterns in cultured cortical networks
title_sort	stimulation triggers endogenous activity patterns in cultured cortical networks
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a52c794d3f9a4807a310597e809bc395
work_keys_str_mv	AT valentinapasquale stimulationtriggersendogenousactivitypatternsinculturedcorticalnetworks AT sergiomartinoia stimulationtriggersendogenousactivitypatternsinculturedcorticalnetworks AT michelachiappalone stimulationtriggersendogenousactivitypatternsinculturedcorticalnetworks
_version_	1718388470083223552

Stimulation triggers endogenous activity patterns in cultured cortical networks

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