Ovarian activation delays in peripubertal ewe lambs infected with Haemonchus contortus can be avoided by supplementing protein in their diets

Abstract Background The ewe lamb nutritional and physiological state interfere with the ovarian environment and fertility. The lack or excess of circulating nutrients reaching the ovary can change its gene expression. A protein deficiency in the blood caused by an Haemonchus contortus abomasal infec...

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Autores principales:	Paula Suarez-Henriques, Camila de Miranda e Silva Chaves, Ricardo Cardoso-Leite, Danielle G. Gomes-Caldas, Luciana Morita-Katiki, Siu Mui Tsai, Helder Louvandini
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Peripubertal ewe Gene expression Ovary Dietetic protein Infection Haemonchus contortus Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/a531efd39463418b8b0535e0a063c36e
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spelling	oai:doaj.org-article:a531efd39463418b8b0535e0a063c36e2021-11-08T10:56:55ZOvarian activation delays in peripubertal ewe lambs infected with Haemonchus contortus can be avoided by supplementing protein in their diets10.1186/s12917-021-03020-71746-6148https://doaj.org/article/a531efd39463418b8b0535e0a063c36e2021-11-01T00:00:00Zhttps://doi.org/10.1186/s12917-021-03020-7https://doaj.org/toc/1746-6148Abstract Background The ewe lamb nutritional and physiological state interfere with the ovarian environment and fertility. The lack or excess of circulating nutrients reaching the ovary can change its gene expression. A protein deficiency in the blood caused by an Haemonchus contortus abomasal infection is detrimental to the organism’s development during puberty. The peripubertal period is a time of intensive growth that requires a high level of nutrients. An essential feature controlling pubertal arousal and female reproductive potential is ovarian follicle growth activation. Protein supplementation improves the sheep’s immune response to helminthic infections. We aimed to determine if supplementing protein in infected ewe lambs’ diet would impact the ovarian environment leading to earlier ovarian follicle activation than in infected not supplemented animals. Methods We fed 18 Santa Ines ewe lambs (Ovis aries) - bred by the same ram - with either 12% protein (Control groups) or 19% protein (Supplemented groups) in their diets. After 35 days of the diet, they were each artificially infected or not with 10,000 Haemonchus contortus L3 larvae. Following 77 days of the diet and 42 days of infection, we surgically collected their left ovaries and examined their genes expression through RNA sequencing. Results We found that protein supplementation in infected animals led to an up-regulation of genes (FDR p-values < 0.05) and biological processes (p-value cut-off = 0.01) linked to meiotic activation in pre-ovulatory follicles and primordial follicle activation, among others. The supplemented not infected animals also up-regulated genes and processes linked to meiosis and others, such as circadian behaviour. The not supplemented animals had these same processes down-regulated while up-regulated processes related to tissue morphogenesis, inflammation and immune response. Conclusion Diet’s protein supplementation of peripubertal infected animals allowed them to express genes related to a more mature ovarian follicle stage than their half-sisters that were not supplemented. These results could be modelling potential effects of the interaction between environmental factors, nutrition and infection on reproductive health. When ovarian activation is achieved in a timely fashion, the ewe may generate more lambs during its reproductive life, increasing sheep breeders’ productivity.Paula Suarez-HenriquesCamila de Miranda e Silva ChavesRicardo Cardoso-LeiteDanielle G. Gomes-CaldasLuciana Morita-KatikiSiu Mui TsaiHelder LouvandiniBMCarticlePeripubertal eweGene expressionOvaryDietetic proteinInfectionHaemonchus contortusVeterinary medicineSF600-1100ENBMC Veterinary Research, Vol 17, Iss 1, Pp 1-24 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Peripubertal ewe Gene expression Ovary Dietetic protein Infection Haemonchus contortus Veterinary medicine SF600-1100
spellingShingle	Peripubertal ewe Gene expression Ovary Dietetic protein Infection Haemonchus contortus Veterinary medicine SF600-1100 Paula Suarez-Henriques Camila de Miranda e Silva Chaves Ricardo Cardoso-Leite Danielle G. Gomes-Caldas Luciana Morita-Katiki Siu Mui Tsai Helder Louvandini Ovarian activation delays in peripubertal ewe lambs infected with Haemonchus contortus can be avoided by supplementing protein in their diets
description	Abstract Background The ewe lamb nutritional and physiological state interfere with the ovarian environment and fertility. The lack or excess of circulating nutrients reaching the ovary can change its gene expression. A protein deficiency in the blood caused by an Haemonchus contortus abomasal infection is detrimental to the organism’s development during puberty. The peripubertal period is a time of intensive growth that requires a high level of nutrients. An essential feature controlling pubertal arousal and female reproductive potential is ovarian follicle growth activation. Protein supplementation improves the sheep’s immune response to helminthic infections. We aimed to determine if supplementing protein in infected ewe lambs’ diet would impact the ovarian environment leading to earlier ovarian follicle activation than in infected not supplemented animals. Methods We fed 18 Santa Ines ewe lambs (Ovis aries) - bred by the same ram - with either 12% protein (Control groups) or 19% protein (Supplemented groups) in their diets. After 35 days of the diet, they were each artificially infected or not with 10,000 Haemonchus contortus L3 larvae. Following 77 days of the diet and 42 days of infection, we surgically collected their left ovaries and examined their genes expression through RNA sequencing. Results We found that protein supplementation in infected animals led to an up-regulation of genes (FDR p-values < 0.05) and biological processes (p-value cut-off = 0.01) linked to meiotic activation in pre-ovulatory follicles and primordial follicle activation, among others. The supplemented not infected animals also up-regulated genes and processes linked to meiosis and others, such as circadian behaviour. The not supplemented animals had these same processes down-regulated while up-regulated processes related to tissue morphogenesis, inflammation and immune response. Conclusion Diet’s protein supplementation of peripubertal infected animals allowed them to express genes related to a more mature ovarian follicle stage than their half-sisters that were not supplemented. These results could be modelling potential effects of the interaction between environmental factors, nutrition and infection on reproductive health. When ovarian activation is achieved in a timely fashion, the ewe may generate more lambs during its reproductive life, increasing sheep breeders’ productivity.
format	article
author	Paula Suarez-Henriques Camila de Miranda e Silva Chaves Ricardo Cardoso-Leite Danielle G. Gomes-Caldas Luciana Morita-Katiki Siu Mui Tsai Helder Louvandini
author_facet	Paula Suarez-Henriques Camila de Miranda e Silva Chaves Ricardo Cardoso-Leite Danielle G. Gomes-Caldas Luciana Morita-Katiki Siu Mui Tsai Helder Louvandini
author_sort	Paula Suarez-Henriques
title	Ovarian activation delays in peripubertal ewe lambs infected with Haemonchus contortus can be avoided by supplementing protein in their diets
title_short	Ovarian activation delays in peripubertal ewe lambs infected with Haemonchus contortus can be avoided by supplementing protein in their diets
title_full	Ovarian activation delays in peripubertal ewe lambs infected with Haemonchus contortus can be avoided by supplementing protein in their diets
title_fullStr	Ovarian activation delays in peripubertal ewe lambs infected with Haemonchus contortus can be avoided by supplementing protein in their diets
title_full_unstemmed	Ovarian activation delays in peripubertal ewe lambs infected with Haemonchus contortus can be avoided by supplementing protein in their diets
title_sort	ovarian activation delays in peripubertal ewe lambs infected with haemonchus contortus can be avoided by supplementing protein in their diets
publisher	BMC
publishDate	2021
url	https://doaj.org/article/a531efd39463418b8b0535e0a063c36e
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Ovarian activation delays in peripubertal ewe lambs infected with Haemonchus contortus can be avoided by supplementing protein in their diets

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