Linoleic and oleic acids enhance cell migration by altering the dynamics of microtubules and the remodeling of the actin cytoskeleton at the leading edge

Abstract Fatty acids (FA) have a multitude of biological actions on living cells. A target of their action is cell motility, a process of critical importance during cancer cell dissemination. Here, we studied the effect of unsaturated FA on ovarian cancer cell migration in vitro and its role in regu...

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Autores principales:	M. Masner, N. Lujea, M. Bisbal, C. Acosta, Patricia Kunda
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a5323f1daed6447da30569a66c3e7c6b
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spelling	oai:doaj.org-article:a5323f1daed6447da30569a66c3e7c6b2021-12-02T17:55:09ZLinoleic and oleic acids enhance cell migration by altering the dynamics of microtubules and the remodeling of the actin cytoskeleton at the leading edge10.1038/s41598-021-94399-82045-2322https://doaj.org/article/a5323f1daed6447da30569a66c3e7c6b2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94399-8https://doaj.org/toc/2045-2322Abstract Fatty acids (FA) have a multitude of biological actions on living cells. A target of their action is cell motility, a process of critical importance during cancer cell dissemination. Here, we studied the effect of unsaturated FA on ovarian cancer cell migration in vitro and its role in regulating cytoskeleton structures that are essential for cell motility. Scratch wound assays on human ovary cancer SKOV-3 cell monolayers revealed that low doses (16 μM) of linoleic acid (LA, 18:2 ω6) and oleic acid (OA; 18:1 ω9) promoted migration, while α-linolenic acid (ALA, 18:3 ω3), showed a migration rate similar to that of the control group. Single cell tracking demonstrated that LA and OA-treated cells migrated faster and were more orientated towards the wound closure than control. In vitro addition of those FA resulted in an increased number, length and protrusion speed of filopodia and also in a prominent and dynamic lamellipodia at the cell leading edge. Using time-lapse video-microscopy and FRAP we observed an increase in both the speed and frequency of actin waves associated with more mobile actin and augmented Rac1 activity. We also observed that FA induced microtubule-organizing center (MTOC)-orientation towards the cell front and affected the dynamics of microtubules (MT) in the direction of cell migration. We propose that environmental cues such as OA and LA present in ascitic fluid, should be taken into account as key factors for the regulation of cell migration.M. MasnerN. LujeaM. BisbalC. AcostaPatricia KundaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q M. Masner N. Lujea M. Bisbal C. Acosta Patricia Kunda Linoleic and oleic acids enhance cell migration by altering the dynamics of microtubules and the remodeling of the actin cytoskeleton at the leading edge
description	Abstract Fatty acids (FA) have a multitude of biological actions on living cells. A target of their action is cell motility, a process of critical importance during cancer cell dissemination. Here, we studied the effect of unsaturated FA on ovarian cancer cell migration in vitro and its role in regulating cytoskeleton structures that are essential for cell motility. Scratch wound assays on human ovary cancer SKOV-3 cell monolayers revealed that low doses (16 μM) of linoleic acid (LA, 18:2 ω6) and oleic acid (OA; 18:1 ω9) promoted migration, while α-linolenic acid (ALA, 18:3 ω3), showed a migration rate similar to that of the control group. Single cell tracking demonstrated that LA and OA-treated cells migrated faster and were more orientated towards the wound closure than control. In vitro addition of those FA resulted in an increased number, length and protrusion speed of filopodia and also in a prominent and dynamic lamellipodia at the cell leading edge. Using time-lapse video-microscopy and FRAP we observed an increase in both the speed and frequency of actin waves associated with more mobile actin and augmented Rac1 activity. We also observed that FA induced microtubule-organizing center (MTOC)-orientation towards the cell front and affected the dynamics of microtubules (MT) in the direction of cell migration. We propose that environmental cues such as OA and LA present in ascitic fluid, should be taken into account as key factors for the regulation of cell migration.
format	article
author	M. Masner N. Lujea M. Bisbal C. Acosta Patricia Kunda
author_facet	M. Masner N. Lujea M. Bisbal C. Acosta Patricia Kunda
author_sort	M. Masner
title	Linoleic and oleic acids enhance cell migration by altering the dynamics of microtubules and the remodeling of the actin cytoskeleton at the leading edge
title_short	Linoleic and oleic acids enhance cell migration by altering the dynamics of microtubules and the remodeling of the actin cytoskeleton at the leading edge
title_full	Linoleic and oleic acids enhance cell migration by altering the dynamics of microtubules and the remodeling of the actin cytoskeleton at the leading edge
title_fullStr	Linoleic and oleic acids enhance cell migration by altering the dynamics of microtubules and the remodeling of the actin cytoskeleton at the leading edge
title_full_unstemmed	Linoleic and oleic acids enhance cell migration by altering the dynamics of microtubules and the remodeling of the actin cytoskeleton at the leading edge
title_sort	linoleic and oleic acids enhance cell migration by altering the dynamics of microtubules and the remodeling of the actin cytoskeleton at the leading edge
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a5323f1daed6447da30569a66c3e7c6b
work_keys_str_mv	AT mmasner linoleicandoleicacidsenhancecellmigrationbyalteringthedynamicsofmicrotubulesandtheremodelingoftheactincytoskeletonattheleadingedge AT nlujea linoleicandoleicacidsenhancecellmigrationbyalteringthedynamicsofmicrotubulesandtheremodelingoftheactincytoskeletonattheleadingedge AT mbisbal linoleicandoleicacidsenhancecellmigrationbyalteringthedynamicsofmicrotubulesandtheremodelingoftheactincytoskeletonattheleadingedge AT cacosta linoleicandoleicacidsenhancecellmigrationbyalteringthedynamicsofmicrotubulesandtheremodelingoftheactincytoskeletonattheleadingedge AT patriciakunda linoleicandoleicacidsenhancecellmigrationbyalteringthedynamicsofmicrotubulesandtheremodelingoftheactincytoskeletonattheleadingedge
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Linoleic and oleic acids enhance cell migration by altering the dynamics of microtubules and the remodeling of the actin cytoskeleton at the leading edge

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