An integrated analysis of miRNA and mRNA expressions in non-small cell lung cancers.

Using DNA microarrays, we generated both mRNA and miRNA expression data from 6 non-small cell lung cancer (NSCLC) tissues and their matching normal control from adjacent tissues to identify potential miRNA markers for diagnostics. We demonstrated that hsa-miR-96 is significantly and consistently up-...

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Autores principales:	Lina Ma, Yanyan Huang, Wangyu Zhu, Shiquan Zhou, Jihang Zhou, Fang Zeng, Xiaoguang Liu, Yongkui Zhang, Jun Yu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a53536dbd3a3429a9ee24f9aeb7f1eb5
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spelling	oai:doaj.org-article:a53536dbd3a3429a9ee24f9aeb7f1eb52021-11-18T07:35:36ZAn integrated analysis of miRNA and mRNA expressions in non-small cell lung cancers.1932-620310.1371/journal.pone.0026502https://doaj.org/article/a53536dbd3a3429a9ee24f9aeb7f1eb52011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22046296/?tool=EBIhttps://doaj.org/toc/1932-6203Using DNA microarrays, we generated both mRNA and miRNA expression data from 6 non-small cell lung cancer (NSCLC) tissues and their matching normal control from adjacent tissues to identify potential miRNA markers for diagnostics. We demonstrated that hsa-miR-96 is significantly and consistently up-regulated in all 6 NSCLCs. We validated this result in an independent set of 35 paired tumors and their adjacent normal tissues, as well as their sera that are collected before surgical resection or chemotherapy, and the results suggested that hsa-miR-96 may play an important role in NSCLC development and has great potential to be used as a noninvasive marker for diagnosing NSCLC. We predicted potential miRNA target mRNAs based on different methods (TargetScan and miRanda). Further classification of miRNA regulated genes based on their relationship with miRNAs revealed that hsa-miR-96 and certain other miRNAs tend to down-regulate their target mRNAs in NSCLC development, which have expression levels permissive to direct interaction between miRNAs and their target mRNAs. In addition, we identified a significant correlation of miRNA regulation with genes coincide with high density of CpG islands, which suggests that miRNA may represent a primary regulatory mechanism governing basic cellular functions and cell differentiations, and such mechanism may be complementary to DNA methylation in repressing or activating gene expression.Lina MaYanyan HuangWangyu ZhuShiquan ZhouJihang ZhouFang ZengXiaoguang LiuYongkui ZhangJun YuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 10, p e26502 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lina Ma Yanyan Huang Wangyu Zhu Shiquan Zhou Jihang Zhou Fang Zeng Xiaoguang Liu Yongkui Zhang Jun Yu An integrated analysis of miRNA and mRNA expressions in non-small cell lung cancers.
description	Using DNA microarrays, we generated both mRNA and miRNA expression data from 6 non-small cell lung cancer (NSCLC) tissues and their matching normal control from adjacent tissues to identify potential miRNA markers for diagnostics. We demonstrated that hsa-miR-96 is significantly and consistently up-regulated in all 6 NSCLCs. We validated this result in an independent set of 35 paired tumors and their adjacent normal tissues, as well as their sera that are collected before surgical resection or chemotherapy, and the results suggested that hsa-miR-96 may play an important role in NSCLC development and has great potential to be used as a noninvasive marker for diagnosing NSCLC. We predicted potential miRNA target mRNAs based on different methods (TargetScan and miRanda). Further classification of miRNA regulated genes based on their relationship with miRNAs revealed that hsa-miR-96 and certain other miRNAs tend to down-regulate their target mRNAs in NSCLC development, which have expression levels permissive to direct interaction between miRNAs and their target mRNAs. In addition, we identified a significant correlation of miRNA regulation with genes coincide with high density of CpG islands, which suggests that miRNA may represent a primary regulatory mechanism governing basic cellular functions and cell differentiations, and such mechanism may be complementary to DNA methylation in repressing or activating gene expression.
format	article
author	Lina Ma Yanyan Huang Wangyu Zhu Shiquan Zhou Jihang Zhou Fang Zeng Xiaoguang Liu Yongkui Zhang Jun Yu
author_facet	Lina Ma Yanyan Huang Wangyu Zhu Shiquan Zhou Jihang Zhou Fang Zeng Xiaoguang Liu Yongkui Zhang Jun Yu
author_sort	Lina Ma
title	An integrated analysis of miRNA and mRNA expressions in non-small cell lung cancers.
title_short	An integrated analysis of miRNA and mRNA expressions in non-small cell lung cancers.
title_full	An integrated analysis of miRNA and mRNA expressions in non-small cell lung cancers.
title_fullStr	An integrated analysis of miRNA and mRNA expressions in non-small cell lung cancers.
title_full_unstemmed	An integrated analysis of miRNA and mRNA expressions in non-small cell lung cancers.
title_sort	integrated analysis of mirna and mrna expressions in non-small cell lung cancers.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/a53536dbd3a3429a9ee24f9aeb7f1eb5
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An integrated analysis of miRNA and mRNA expressions in non-small cell lung cancers.

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