The O-Antigen Epitope Governs Susceptibility to Colistin in <named-content content-type="genus-species">Salmonella enterica</named-content>

ABSTRACT Group D and group B Salmonella enterica serovars differ in their susceptibility to colistin with the former frequently intrinsically resistant (MIC > 2 μg/ml); however, the mechanism has not been described. Here, we show that the O-antigen epitope in group D Salmonella governs the levels...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Vito Ricci, Dexian Zhang, Christopher Teale, Laura J. V. Piddock
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	whole-genome sequencing LPS Salmonella Enteritidis lipopolysaccharide Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/a5408f768a5543cfb08dcda81aaf3e3b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a5408f768a5543cfb08dcda81aaf3e3b
record_format	dspace
spelling	oai:doaj.org-article:a5408f768a5543cfb08dcda81aaf3e3b2021-11-15T15:56:58ZThe O-Antigen Epitope Governs Susceptibility to Colistin in <named-content content-type="genus-species">Salmonella enterica</named-content>10.1128/mBio.02831-192150-7511https://doaj.org/article/a5408f768a5543cfb08dcda81aaf3e3b2020-02-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.02831-19https://doaj.org/toc/2150-7511ABSTRACT Group D and group B Salmonella enterica serovars differ in their susceptibility to colistin with the former frequently intrinsically resistant (MIC > 2 μg/ml); however, the mechanism has not been described. Here, we show that the O-antigen epitope in group D Salmonella governs the levels of colistin susceptibility. Substitution of the rfbJ gene in a group B Salmonella with the rfbSE genes from a group D Salmonella conferred a decrease in susceptibility to colistin. The presence of dideoxyhexose, abequose, and the deoxymannose, tyvelose, differentiate the Salmonella group B and group D O antigens, respectively. We hypothesize that the subtle difference between abequose and tyvelose hinders the colistin molecule from reaching its target. Whole-genome sequencing also revealed that increased colistin susceptibility in a group D Salmonella veterinary isolate was due to a defect in the O-antigen polymerase protein, Rfc. This study shows that two different mechanisms that influence the presence and composition of O antigens affect colistin susceptibility in Salmonella enterica. IMPORTANCE Some serovars of Salmonella, namely, those belonging to group D, appear to show a degree of intrinsic resistance to colistin. This observed intrinsic colistin resistance is of concern since this last-resort drug might no longer be effective for treating severe human infections with the most common Salmonella serovar, Salmonella enterica serovar Enteritidis. Here, we show that the O-antigen epitope in group D Salmonella governs the levels of colistin susceptibility. Using whole-genome sequencing, we also revealed that increased colistin susceptibility in a group D Salmonella veterinary isolate was due to a defect in the O-antigen polymerase protein, Rfc. In summary, we show that two different mechanisms that influence the presence and composition of O antigens affect colistin susceptibility in Salmonella enterica.Vito RicciDexian ZhangChristopher TealeLaura J. V. PiddockAmerican Society for Microbiologyarticlewhole-genome sequencingLPSSalmonella EnteritidislipopolysaccharideMicrobiologyQR1-502ENmBio, Vol 11, Iss 1 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	whole-genome sequencing LPS Salmonella Enteritidis lipopolysaccharide Microbiology QR1-502
spellingShingle	whole-genome sequencing LPS Salmonella Enteritidis lipopolysaccharide Microbiology QR1-502 Vito Ricci Dexian Zhang Christopher Teale Laura J. V. Piddock The O-Antigen Epitope Governs Susceptibility to Colistin in <named-content content-type="genus-species">Salmonella enterica</named-content>
description	ABSTRACT Group D and group B Salmonella enterica serovars differ in their susceptibility to colistin with the former frequently intrinsically resistant (MIC > 2 μg/ml); however, the mechanism has not been described. Here, we show that the O-antigen epitope in group D Salmonella governs the levels of colistin susceptibility. Substitution of the rfbJ gene in a group B Salmonella with the rfbSE genes from a group D Salmonella conferred a decrease in susceptibility to colistin. The presence of dideoxyhexose, abequose, and the deoxymannose, tyvelose, differentiate the Salmonella group B and group D O antigens, respectively. We hypothesize that the subtle difference between abequose and tyvelose hinders the colistin molecule from reaching its target. Whole-genome sequencing also revealed that increased colistin susceptibility in a group D Salmonella veterinary isolate was due to a defect in the O-antigen polymerase protein, Rfc. This study shows that two different mechanisms that influence the presence and composition of O antigens affect colistin susceptibility in Salmonella enterica. IMPORTANCE Some serovars of Salmonella, namely, those belonging to group D, appear to show a degree of intrinsic resistance to colistin. This observed intrinsic colistin resistance is of concern since this last-resort drug might no longer be effective for treating severe human infections with the most common Salmonella serovar, Salmonella enterica serovar Enteritidis. Here, we show that the O-antigen epitope in group D Salmonella governs the levels of colistin susceptibility. Using whole-genome sequencing, we also revealed that increased colistin susceptibility in a group D Salmonella veterinary isolate was due to a defect in the O-antigen polymerase protein, Rfc. In summary, we show that two different mechanisms that influence the presence and composition of O antigens affect colistin susceptibility in Salmonella enterica.
format	article
author	Vito Ricci Dexian Zhang Christopher Teale Laura J. V. Piddock
author_facet	Vito Ricci Dexian Zhang Christopher Teale Laura J. V. Piddock
author_sort	Vito Ricci
title	The O-Antigen Epitope Governs Susceptibility to Colistin in <named-content content-type="genus-species">Salmonella enterica</named-content>
title_short	The O-Antigen Epitope Governs Susceptibility to Colistin in <named-content content-type="genus-species">Salmonella enterica</named-content>
title_full	The O-Antigen Epitope Governs Susceptibility to Colistin in <named-content content-type="genus-species">Salmonella enterica</named-content>
title_fullStr	The O-Antigen Epitope Governs Susceptibility to Colistin in <named-content content-type="genus-species">Salmonella enterica</named-content>
title_full_unstemmed	The O-Antigen Epitope Governs Susceptibility to Colistin in <named-content content-type="genus-species">Salmonella enterica</named-content>
title_sort	o-antigen epitope governs susceptibility to colistin in <named-content content-type="genus-species">salmonella enterica</named-content>
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/a5408f768a5543cfb08dcda81aaf3e3b
work_keys_str_mv	AT vitoricci theoantigenepitopegovernssusceptibilitytocolistininnamedcontentcontenttypegenusspeciessalmonellaentericanamedcontent AT dexianzhang theoantigenepitopegovernssusceptibilitytocolistininnamedcontentcontenttypegenusspeciessalmonellaentericanamedcontent AT christopherteale theoantigenepitopegovernssusceptibilitytocolistininnamedcontentcontenttypegenusspeciessalmonellaentericanamedcontent AT laurajvpiddock theoantigenepitopegovernssusceptibilitytocolistininnamedcontentcontenttypegenusspeciessalmonellaentericanamedcontent AT vitoricci oantigenepitopegovernssusceptibilitytocolistininnamedcontentcontenttypegenusspeciessalmonellaentericanamedcontent AT dexianzhang oantigenepitopegovernssusceptibilitytocolistininnamedcontentcontenttypegenusspeciessalmonellaentericanamedcontent AT christopherteale oantigenepitopegovernssusceptibilitytocolistininnamedcontentcontenttypegenusspeciessalmonellaentericanamedcontent AT laurajvpiddock oantigenepitopegovernssusceptibilitytocolistininnamedcontentcontenttypegenusspeciessalmonellaentericanamedcontent
_version_	1718427110298615808

The O-Antigen Epitope Governs Susceptibility to Colistin in <named-content content-type="genus-species">Salmonella enterica</named-content>

Ejemplares similares