Involvement of orexin neurons in fasting- and central adenosine-induced hypothermia

Abstract We examined whether orexin neurons might play a protective role against fasting- and adenosine-induced hypothermia. We first measured body temperature (BT) in orexin neuron-ablated (ORX-AB) mice and wild-type (WT) controls during 24 hours of fasting. As expected, the magnitude of BT drop an...

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Autores principales:	Takahiro Futatsuki, Akira Yamashita, Khairunnisa Novita Ikbar, Akihiro Yamanaka, Kazunori Arita, Yasuyuki Kakihana, Tomoyuki Kuwaki
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a54f9a0cdb6c49f09b18b49d25e88427
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spelling	oai:doaj.org-article:a54f9a0cdb6c49f09b18b49d25e884272021-12-02T15:08:03ZInvolvement of orexin neurons in fasting- and central adenosine-induced hypothermia10.1038/s41598-018-21252-w2045-2322https://doaj.org/article/a54f9a0cdb6c49f09b18b49d25e884272018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-21252-whttps://doaj.org/toc/2045-2322Abstract We examined whether orexin neurons might play a protective role against fasting- and adenosine-induced hypothermia. We first measured body temperature (BT) in orexin neuron-ablated (ORX-AB) mice and wild-type (WT) controls during 24 hours of fasting. As expected, the magnitude of BT drop and the length of time suffering from hypothermia were greater in ORX-AB mice than in WT mice. Orexin neurons were active just before onset of hypothermia and during the recovery period as revealed by calcium imaging in vivo using G-CaMP. We next examined adenosine-induced hypothermia via an intracerebroventricular administration of an adenosine A1 receptor agonist, N6-cyclohexyladenosine (CHA), which induced hypothermia in both ORX-AB and WT mice. The dose of CHA required to initiate a hypothermic response in ORX-AB mice was more than 10 times larger than the dose for WT mice. Once hypothermia was established, the recovery was seemingly slower in ORX-AB mice. Activation of orexin neurons during the recovery phase was confirmed by immunohistochemistry for c-Fos. We propose that orexin neurons play dual roles (enhancer in the induction phase and compensator during the recovery phase) in adenosine-induced hypothermia and a protective/compensatory role in fasting-induced hypothermia.Takahiro FutatsukiAkira YamashitaKhairunnisa Novita IkbarAkihiro YamanakaKazunori AritaYasuyuki KakihanaTomoyuki KuwakiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Takahiro Futatsuki Akira Yamashita Khairunnisa Novita Ikbar Akihiro Yamanaka Kazunori Arita Yasuyuki Kakihana Tomoyuki Kuwaki Involvement of orexin neurons in fasting- and central adenosine-induced hypothermia
description	Abstract We examined whether orexin neurons might play a protective role against fasting- and adenosine-induced hypothermia. We first measured body temperature (BT) in orexin neuron-ablated (ORX-AB) mice and wild-type (WT) controls during 24 hours of fasting. As expected, the magnitude of BT drop and the length of time suffering from hypothermia were greater in ORX-AB mice than in WT mice. Orexin neurons were active just before onset of hypothermia and during the recovery period as revealed by calcium imaging in vivo using G-CaMP. We next examined adenosine-induced hypothermia via an intracerebroventricular administration of an adenosine A1 receptor agonist, N6-cyclohexyladenosine (CHA), which induced hypothermia in both ORX-AB and WT mice. The dose of CHA required to initiate a hypothermic response in ORX-AB mice was more than 10 times larger than the dose for WT mice. Once hypothermia was established, the recovery was seemingly slower in ORX-AB mice. Activation of orexin neurons during the recovery phase was confirmed by immunohistochemistry for c-Fos. We propose that orexin neurons play dual roles (enhancer in the induction phase and compensator during the recovery phase) in adenosine-induced hypothermia and a protective/compensatory role in fasting-induced hypothermia.
format	article
author	Takahiro Futatsuki Akira Yamashita Khairunnisa Novita Ikbar Akihiro Yamanaka Kazunori Arita Yasuyuki Kakihana Tomoyuki Kuwaki
author_facet	Takahiro Futatsuki Akira Yamashita Khairunnisa Novita Ikbar Akihiro Yamanaka Kazunori Arita Yasuyuki Kakihana Tomoyuki Kuwaki
author_sort	Takahiro Futatsuki
title	Involvement of orexin neurons in fasting- and central adenosine-induced hypothermia
title_short	Involvement of orexin neurons in fasting- and central adenosine-induced hypothermia
title_full	Involvement of orexin neurons in fasting- and central adenosine-induced hypothermia
title_fullStr	Involvement of orexin neurons in fasting- and central adenosine-induced hypothermia
title_full_unstemmed	Involvement of orexin neurons in fasting- and central adenosine-induced hypothermia
title_sort	involvement of orexin neurons in fasting- and central adenosine-induced hypothermia
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/a54f9a0cdb6c49f09b18b49d25e88427
work_keys_str_mv	AT takahirofutatsuki involvementoforexinneuronsinfastingandcentraladenosineinducedhypothermia AT akirayamashita involvementoforexinneuronsinfastingandcentraladenosineinducedhypothermia AT khairunnisanovitaikbar involvementoforexinneuronsinfastingandcentraladenosineinducedhypothermia AT akihiroyamanaka involvementoforexinneuronsinfastingandcentraladenosineinducedhypothermia AT kazunoriarita involvementoforexinneuronsinfastingandcentraladenosineinducedhypothermia AT yasuyukikakihana involvementoforexinneuronsinfastingandcentraladenosineinducedhypothermia AT tomoyukikuwaki involvementoforexinneuronsinfastingandcentraladenosineinducedhypothermia
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Involvement of orexin neurons in fasting- and central adenosine-induced hypothermia

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