Effect of surface organic coatings of cellulose nanocrystals on the viability of mammalian cell lines

Ambar S Jimenez,1 Francesca Jaramillo,1 Usha D Hemraz,2 Yaman Boluk,3 Karina Ckless,1 Rajesh Sunasee1 1Department of Chemistry, State University of New York at Plattsburgh, Plattsburgh, NY, USA; 2National Research Council, Montreal, QC, Canada, 3Department of Civil & Environmental Engineerin...

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Autores principales:	Jimenez AS, Jaramillo F, Hemraz UD, Boluk Y, Ckless K, Sunasee R
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	cellulose nanocrystals surface coating cell viability MTT LDH Medical technology R855-855.5 Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/a558f352fe084cecbbb25f008813ef05
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spelling	oai:doaj.org-article:a558f352fe084cecbbb25f008813ef052021-12-02T04:41:35ZEffect of surface organic coatings of cellulose nanocrystals on the viability of mammalian cell lines1177-8903https://doaj.org/article/a558f352fe084cecbbb25f008813ef052017-09-01T00:00:00Zhttps://www.dovepress.com/effect-of-surface-organic-coatings-of-cellulose-nanocrystals-on-the-vi-peer-reviewed-article-NSAhttps://doaj.org/toc/1177-8903Ambar S Jimenez,1 Francesca Jaramillo,1 Usha D Hemraz,2 Yaman Boluk,3 Karina Ckless,1 Rajesh Sunasee1 1Department of Chemistry, State University of New York at Plattsburgh, Plattsburgh, NY, USA; 2National Research Council, Montreal, QC, Canada, 3Department of Civil & Environmental Engineering, University of Alberta and National Institute for Nanotechnology, National Research Council, Edmonton, AB, Canada Abstract: Cellulose nanocrystals (CNCs) have emerged as promising candidates for a number of bio-applications. Surface modification of CNCs continues to gain significant research interest as it imparts new properties to the surface of the nanocrystals for the design of multifunctional CNCs-based materials. A small chemical surface modification can potentially lead to drastic behavioral changes of cell-material interactions thereby affecting the intended bio-application. In this work, unmodified CNCs were covalently decorated with four different organic moieties such as a diaminobutane fragment, a cyclic oligosaccharide (β-cyclodextrin), a thermoresponsive polymer (poly[N-isopropylacrylamide]), and a cationic aminomethacrylamide-based polymer using different synthetic covalent methods. The effect of surface coatings of CNCs and the respective dose-response of the above organic moieties on the cell viability were evaluated on mammalian cell cultures (J774A.1 and MFC-7), using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase assays. Overall, the results indicated that cells exposed to surface-coated CNCs for 24 h did not display major changes in cell viability, membrane permeability as well as cell morphology. However, with longer exposure, all these parameters were somewhat affected, which appears not to be correlated with either anionic or cationic surface coatings of CNCs used in this study. Keywords: cellulose nanocrystals, surface coating, cell viability, MTT, LDHJimenez ASJaramillo FHemraz UDBoluk YCkless KSunasee RDove Medical Pressarticlecellulose nanocrystalssurface coatingcell viabilityMTTLDHMedical technologyR855-855.5Chemical technologyTP1-1185ENNanotechnology, Science and Applications, Vol Volume 10, Pp 123-136 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	cellulose nanocrystals surface coating cell viability MTT LDH Medical technology R855-855.5 Chemical technology TP1-1185
spellingShingle	cellulose nanocrystals surface coating cell viability MTT LDH Medical technology R855-855.5 Chemical technology TP1-1185 Jimenez AS Jaramillo F Hemraz UD Boluk Y Ckless K Sunasee R Effect of surface organic coatings of cellulose nanocrystals on the viability of mammalian cell lines
description	Ambar S Jimenez,1 Francesca Jaramillo,1 Usha D Hemraz,2 Yaman Boluk,3 Karina Ckless,1 Rajesh Sunasee1 1Department of Chemistry, State University of New York at Plattsburgh, Plattsburgh, NY, USA; 2National Research Council, Montreal, QC, Canada, 3Department of Civil & Environmental Engineering, University of Alberta and National Institute for Nanotechnology, National Research Council, Edmonton, AB, Canada Abstract: Cellulose nanocrystals (CNCs) have emerged as promising candidates for a number of bio-applications. Surface modification of CNCs continues to gain significant research interest as it imparts new properties to the surface of the nanocrystals for the design of multifunctional CNCs-based materials. A small chemical surface modification can potentially lead to drastic behavioral changes of cell-material interactions thereby affecting the intended bio-application. In this work, unmodified CNCs were covalently decorated with four different organic moieties such as a diaminobutane fragment, a cyclic oligosaccharide (β-cyclodextrin), a thermoresponsive polymer (poly[N-isopropylacrylamide]), and a cationic aminomethacrylamide-based polymer using different synthetic covalent methods. The effect of surface coatings of CNCs and the respective dose-response of the above organic moieties on the cell viability were evaluated on mammalian cell cultures (J774A.1 and MFC-7), using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase assays. Overall, the results indicated that cells exposed to surface-coated CNCs for 24 h did not display major changes in cell viability, membrane permeability as well as cell morphology. However, with longer exposure, all these parameters were somewhat affected, which appears not to be correlated with either anionic or cationic surface coatings of CNCs used in this study. Keywords: cellulose nanocrystals, surface coating, cell viability, MTT, LDH
format	article
author	Jimenez AS Jaramillo F Hemraz UD Boluk Y Ckless K Sunasee R
author_facet	Jimenez AS Jaramillo F Hemraz UD Boluk Y Ckless K Sunasee R
author_sort	Jimenez AS
title	Effect of surface organic coatings of cellulose nanocrystals on the viability of mammalian cell lines
title_short	Effect of surface organic coatings of cellulose nanocrystals on the viability of mammalian cell lines
title_full	Effect of surface organic coatings of cellulose nanocrystals on the viability of mammalian cell lines
title_fullStr	Effect of surface organic coatings of cellulose nanocrystals on the viability of mammalian cell lines
title_full_unstemmed	Effect of surface organic coatings of cellulose nanocrystals on the viability of mammalian cell lines
title_sort	effect of surface organic coatings of cellulose nanocrystals on the viability of mammalian cell lines
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/a558f352fe084cecbbb25f008813ef05
work_keys_str_mv	AT jimenezas effectofsurfaceorganiccoatingsofcellulosenanocrystalsontheviabilityofmammaliancelllines AT jaramillof effectofsurfaceorganiccoatingsofcellulosenanocrystalsontheviabilityofmammaliancelllines AT hemrazud effectofsurfaceorganiccoatingsofcellulosenanocrystalsontheviabilityofmammaliancelllines AT boluky effectofsurfaceorganiccoatingsofcellulosenanocrystalsontheviabilityofmammaliancelllines AT cklessk effectofsurfaceorganiccoatingsofcellulosenanocrystalsontheviabilityofmammaliancelllines AT sunaseer effectofsurfaceorganiccoatingsofcellulosenanocrystalsontheviabilityofmammaliancelllines
_version_	1718401141552709632

Effect of surface organic coatings of cellulose nanocrystals on the viability of mammalian cell lines

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