Cu-bearing stainless steel reduces cytotoxicity and crystals adhesion after ureteral epithelial cells exposing to calcium oxalate monohydrate

Abstract Calcium oxalate monohydrate (COM), which is the main component of encrustation, may result in cell membrane injury. In addition, cellular damage is suggested to be the primary event attributing to COM crystal binding. To study the interaction between cells and crystals after incubating with...

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Autores principales:	Zhiqiang Cao, Jing Zhao, Ke Yang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Ureteral Epithelial Cells (UECs) Crystal Adhesion Calcium Oxalate Monohydrate (COM) Cu-Zn Superoxide Dismutase (CuZnSOD) Crystal Binding Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a55be4e834e040df88e0732b70a49a14
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spelling	oai:doaj.org-article:a55be4e834e040df88e0732b70a49a142021-12-02T15:08:43ZCu-bearing stainless steel reduces cytotoxicity and crystals adhesion after ureteral epithelial cells exposing to calcium oxalate monohydrate10.1038/s41598-018-32388-02045-2322https://doaj.org/article/a55be4e834e040df88e0732b70a49a142018-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-32388-0https://doaj.org/toc/2045-2322Abstract Calcium oxalate monohydrate (COM), which is the main component of encrustation, may result in cell membrane injury. In addition, cellular damage is suggested to be the primary event attributing to COM crystal binding. To study the interaction between cells and crystals after incubating with a Cu-bearing stainless steel (316L-Cu SS), MTS and flow cytometric analyses were used to assess the cellular responses. The results confirmed that 316L-Cu SS could inhibit cytotoxicity and cellular apoptosis of ureteral epithelial cells (UECs) after COM treatment. Furthermore, molecular expressions of Cu/Zn superoxide dismutase (CuZnSOD), which were evaluated by western blot analysis and real-time quantitative PCR (qPCR), indicated that 316L-Cu SS could inhibit the oxidative stress attributing to up-regulating of CuZnSOD. Moreover, the crystal adhesion cytokine CD44 was examined with western blot and qPCR, and the corresponding hyaluronic (HA) secreted into the medium was measured by enzyme-linked immunosorbent assay (ELISA). All results were confirmed that the expressions of cells cultured with 316L-Cu SS were down-regulated, demonstrating the inhibitory performance of 316L-Cu SS against crystal adhesion.Zhiqiang CaoJing ZhaoKe YangNature PortfolioarticleUreteral Epithelial Cells (UECs)Crystal AdhesionCalcium Oxalate Monohydrate (COM)Cu-Zn Superoxide Dismutase (CuZnSOD)Crystal BindingMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-8 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ureteral Epithelial Cells (UECs) Crystal Adhesion Calcium Oxalate Monohydrate (COM) Cu-Zn Superoxide Dismutase (CuZnSOD) Crystal Binding Medicine R Science Q
spellingShingle	Ureteral Epithelial Cells (UECs) Crystal Adhesion Calcium Oxalate Monohydrate (COM) Cu-Zn Superoxide Dismutase (CuZnSOD) Crystal Binding Medicine R Science Q Zhiqiang Cao Jing Zhao Ke Yang Cu-bearing stainless steel reduces cytotoxicity and crystals adhesion after ureteral epithelial cells exposing to calcium oxalate monohydrate
description	Abstract Calcium oxalate monohydrate (COM), which is the main component of encrustation, may result in cell membrane injury. In addition, cellular damage is suggested to be the primary event attributing to COM crystal binding. To study the interaction between cells and crystals after incubating with a Cu-bearing stainless steel (316L-Cu SS), MTS and flow cytometric analyses were used to assess the cellular responses. The results confirmed that 316L-Cu SS could inhibit cytotoxicity and cellular apoptosis of ureteral epithelial cells (UECs) after COM treatment. Furthermore, molecular expressions of Cu/Zn superoxide dismutase (CuZnSOD), which were evaluated by western blot analysis and real-time quantitative PCR (qPCR), indicated that 316L-Cu SS could inhibit the oxidative stress attributing to up-regulating of CuZnSOD. Moreover, the crystal adhesion cytokine CD44 was examined with western blot and qPCR, and the corresponding hyaluronic (HA) secreted into the medium was measured by enzyme-linked immunosorbent assay (ELISA). All results were confirmed that the expressions of cells cultured with 316L-Cu SS were down-regulated, demonstrating the inhibitory performance of 316L-Cu SS against crystal adhesion.
format	article
author	Zhiqiang Cao Jing Zhao Ke Yang
author_facet	Zhiqiang Cao Jing Zhao Ke Yang
author_sort	Zhiqiang Cao
title	Cu-bearing stainless steel reduces cytotoxicity and crystals adhesion after ureteral epithelial cells exposing to calcium oxalate monohydrate
title_short	Cu-bearing stainless steel reduces cytotoxicity and crystals adhesion after ureteral epithelial cells exposing to calcium oxalate monohydrate
title_full	Cu-bearing stainless steel reduces cytotoxicity and crystals adhesion after ureteral epithelial cells exposing to calcium oxalate monohydrate
title_fullStr	Cu-bearing stainless steel reduces cytotoxicity and crystals adhesion after ureteral epithelial cells exposing to calcium oxalate monohydrate
title_full_unstemmed	Cu-bearing stainless steel reduces cytotoxicity and crystals adhesion after ureteral epithelial cells exposing to calcium oxalate monohydrate
title_sort	cu-bearing stainless steel reduces cytotoxicity and crystals adhesion after ureteral epithelial cells exposing to calcium oxalate monohydrate
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/a55be4e834e040df88e0732b70a49a14
work_keys_str_mv	AT zhiqiangcao cubearingstainlesssteelreducescytotoxicityandcrystalsadhesionafterureteralepithelialcellsexposingtocalciumoxalatemonohydrate AT jingzhao cubearingstainlesssteelreducescytotoxicityandcrystalsadhesionafterureteralepithelialcellsexposingtocalciumoxalatemonohydrate AT keyang cubearingstainlesssteelreducescytotoxicityandcrystalsadhesionafterureteralepithelialcellsexposingtocalciumoxalatemonohydrate
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Cu-bearing stainless steel reduces cytotoxicity and crystals adhesion after ureteral epithelial cells exposing to calcium oxalate monohydrate

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