3D bioprinting of tissue-specific osteoblasts and endothelial cells to model the human jawbone

Abstract Jawbone differs from other bones in many aspects, including its developmental origin and the occurrence of jawbone-specific diseases like MRONJ (medication-related osteonecrosis of the jaw). Although there is a strong need, adequate in vitro models of this unique environment are sparse to d...

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Autores principales:	Anna-Klara Amler, Alexander Thomas, Selin Tüzüner, Tobias Lam, Michel-Andreas Geiger, Anna-Elisabeth Kreuder, Chris Palmer, Susanne Nahles, Roland Lauster, Lutz Kloke
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a55c496c29ff4ecdb9bf2348d395f254
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spelling	oai:doaj.org-article:a55c496c29ff4ecdb9bf2348d395f2542021-12-02T13:19:30Z3D bioprinting of tissue-specific osteoblasts and endothelial cells to model the human jawbone10.1038/s41598-021-84483-42045-2322https://doaj.org/article/a55c496c29ff4ecdb9bf2348d395f2542021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84483-4https://doaj.org/toc/2045-2322Abstract Jawbone differs from other bones in many aspects, including its developmental origin and the occurrence of jawbone-specific diseases like MRONJ (medication-related osteonecrosis of the jaw). Although there is a strong need, adequate in vitro models of this unique environment are sparse to date. While previous approaches are reliant e.g. on scaffolds or spheroid culture, 3D bioprinting enables free-form fabrication of complex living tissue structures. In the present work, production of human jawbone models was realised via projection-based stereolithography. Constructs were bioprinted containing primary jawbone-derived osteoblasts and vasculature-like channel structures optionally harbouring primary endothelial cells. After 28 days of cultivation in growth medium or osteogenic medium, expression of cell type-specific markers was confirmed on both the RNA and protein level, while prints maintained their overall structure. Survival of endothelial cells in the printed channels, co-cultured with osteoblasts in medium without supplementation of endothelial growth factors, was demonstrated. Constructs showed not only mineralisation, being one of the characteristics of osteoblasts, but also hinted at differentiation to an osteocyte phenotype. These results indicate the successful biofabrication of an in vitro model of the human jawbone, which presents key features of this special bone entity and hence appears promising for application in jawbone-specific research.Anna-Klara AmlerAlexander ThomasSelin TüzünerTobias LamMichel-Andreas GeigerAnna-Elisabeth KreuderChris PalmerSusanne NahlesRoland LausterLutz KlokeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anna-Klara Amler Alexander Thomas Selin Tüzüner Tobias Lam Michel-Andreas Geiger Anna-Elisabeth Kreuder Chris Palmer Susanne Nahles Roland Lauster Lutz Kloke 3D bioprinting of tissue-specific osteoblasts and endothelial cells to model the human jawbone
description	Abstract Jawbone differs from other bones in many aspects, including its developmental origin and the occurrence of jawbone-specific diseases like MRONJ (medication-related osteonecrosis of the jaw). Although there is a strong need, adequate in vitro models of this unique environment are sparse to date. While previous approaches are reliant e.g. on scaffolds or spheroid culture, 3D bioprinting enables free-form fabrication of complex living tissue structures. In the present work, production of human jawbone models was realised via projection-based stereolithography. Constructs were bioprinted containing primary jawbone-derived osteoblasts and vasculature-like channel structures optionally harbouring primary endothelial cells. After 28 days of cultivation in growth medium or osteogenic medium, expression of cell type-specific markers was confirmed on both the RNA and protein level, while prints maintained their overall structure. Survival of endothelial cells in the printed channels, co-cultured with osteoblasts in medium without supplementation of endothelial growth factors, was demonstrated. Constructs showed not only mineralisation, being one of the characteristics of osteoblasts, but also hinted at differentiation to an osteocyte phenotype. These results indicate the successful biofabrication of an in vitro model of the human jawbone, which presents key features of this special bone entity and hence appears promising for application in jawbone-specific research.
format	article
author	Anna-Klara Amler Alexander Thomas Selin Tüzüner Tobias Lam Michel-Andreas Geiger Anna-Elisabeth Kreuder Chris Palmer Susanne Nahles Roland Lauster Lutz Kloke
author_facet	Anna-Klara Amler Alexander Thomas Selin Tüzüner Tobias Lam Michel-Andreas Geiger Anna-Elisabeth Kreuder Chris Palmer Susanne Nahles Roland Lauster Lutz Kloke
author_sort	Anna-Klara Amler
title	3D bioprinting of tissue-specific osteoblasts and endothelial cells to model the human jawbone
title_short	3D bioprinting of tissue-specific osteoblasts and endothelial cells to model the human jawbone
title_full	3D bioprinting of tissue-specific osteoblasts and endothelial cells to model the human jawbone
title_fullStr	3D bioprinting of tissue-specific osteoblasts and endothelial cells to model the human jawbone
title_full_unstemmed	3D bioprinting of tissue-specific osteoblasts and endothelial cells to model the human jawbone
title_sort	3d bioprinting of tissue-specific osteoblasts and endothelial cells to model the human jawbone
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a55c496c29ff4ecdb9bf2348d395f254
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3D bioprinting of tissue-specific osteoblasts and endothelial cells to model the human jawbone

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