Molecular fingerprinting of bovine mastitis-associated Staphylococcus aureus isolates from India

Abstract Staphylococcus aureus is a major etiological agent of clinical and subclinical bovine mastitis. Owing to the mostly backyard dairy practices, we hypothesized that genetic diversity among mastitis-associated S. aureus from India would be high, and investigated 166 isolates obtained mostly fr...

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Autores principales:	Madhavi Annamanedi, P. Sheela, Srinivasaiah Sundareshan, Shrikrishna Isloor, Priya Gupta, Pagala Jasmeen, Madhuranjana Gargi, Sathi Mallick, Nagendra R. Hegde
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a5611aac6ced45c58f5b7f33dc3aa8cf
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spelling	oai:doaj.org-article:a5611aac6ced45c58f5b7f33dc3aa8cf2021-12-02T16:06:41ZMolecular fingerprinting of bovine mastitis-associated Staphylococcus aureus isolates from India10.1038/s41598-021-94760-x2045-2322https://doaj.org/article/a5611aac6ced45c58f5b7f33dc3aa8cf2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94760-xhttps://doaj.org/toc/2045-2322Abstract Staphylococcus aureus is a major etiological agent of clinical and subclinical bovine mastitis. Owing to the mostly backyard dairy practices, we hypothesized that genetic diversity among mastitis-associated S. aureus from India would be high, and investigated 166 isolates obtained mostly from the Southern State of Karnataka, but also from a few other states. The results revealed (a) 8 to 13 fragments in pulsed-field gel electrophoresis (PFGE), forming 31 distinct patterns, and (b) 34 spa types, of which three (t17680, t18314, and t18320) were newly identified. Multi-locus sequencing typing (MLST) identified 39 sequence types (STs), with ST2454 (34.4%) and ST2459 (24%) being the most commonly represented, which clustered to clonal complexes (CC) CC9 and CC97, respectively; 12 STs were newly identified. Thirty-four (20.5%) of the 166 isolates displayed oxacillin resistance. On the other hand, whereas none were mecC+, 44 (26.5%) isolates were mecA+, with a predominance of SCCmecIVb (26/32 isolates, others being untypeable); 24 isolates (14.46%) were oxacillin-susceptible methicillin-resistant S. aureus (OS-MRSA; mecA+ but OS). Integrated analysis revealed that CC9-ST2454- and CC97-ST2459-SCCmecIVb were the predominant MRSA, although the distribution of CC9 and CC97 was similar between methicillin-resistant and -susceptible isolates. By PCR, 56.25%, 28.75% and 47.5% of the 166 isolates were positive for hlg, tsst and pvl genes, respectively. Our results, for the first time describe the application of a combination of various molecular methods to bovine mastitis-associated S. aureus isolates from India, corroborate the worldwide distribution of CC97 and CC9, and suggest pathogenic potential of the isolates.Madhavi AnnamanediP. SheelaSrinivasaiah SundareshanShrikrishna IsloorPriya GuptaPagala JasmeenMadhuranjana GargiSathi MallickNagendra R. HegdeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Madhavi Annamanedi P. Sheela Srinivasaiah Sundareshan Shrikrishna Isloor Priya Gupta Pagala Jasmeen Madhuranjana Gargi Sathi Mallick Nagendra R. Hegde Molecular fingerprinting of bovine mastitis-associated Staphylococcus aureus isolates from India
description	Abstract Staphylococcus aureus is a major etiological agent of clinical and subclinical bovine mastitis. Owing to the mostly backyard dairy practices, we hypothesized that genetic diversity among mastitis-associated S. aureus from India would be high, and investigated 166 isolates obtained mostly from the Southern State of Karnataka, but also from a few other states. The results revealed (a) 8 to 13 fragments in pulsed-field gel electrophoresis (PFGE), forming 31 distinct patterns, and (b) 34 spa types, of which three (t17680, t18314, and t18320) were newly identified. Multi-locus sequencing typing (MLST) identified 39 sequence types (STs), with ST2454 (34.4%) and ST2459 (24%) being the most commonly represented, which clustered to clonal complexes (CC) CC9 and CC97, respectively; 12 STs were newly identified. Thirty-four (20.5%) of the 166 isolates displayed oxacillin resistance. On the other hand, whereas none were mecC+, 44 (26.5%) isolates were mecA+, with a predominance of SCCmecIVb (26/32 isolates, others being untypeable); 24 isolates (14.46%) were oxacillin-susceptible methicillin-resistant S. aureus (OS-MRSA; mecA+ but OS). Integrated analysis revealed that CC9-ST2454- and CC97-ST2459-SCCmecIVb were the predominant MRSA, although the distribution of CC9 and CC97 was similar between methicillin-resistant and -susceptible isolates. By PCR, 56.25%, 28.75% and 47.5% of the 166 isolates were positive for hlg, tsst and pvl genes, respectively. Our results, for the first time describe the application of a combination of various molecular methods to bovine mastitis-associated S. aureus isolates from India, corroborate the worldwide distribution of CC97 and CC9, and suggest pathogenic potential of the isolates.
format	article
author	Madhavi Annamanedi P. Sheela Srinivasaiah Sundareshan Shrikrishna Isloor Priya Gupta Pagala Jasmeen Madhuranjana Gargi Sathi Mallick Nagendra R. Hegde
author_facet	Madhavi Annamanedi P. Sheela Srinivasaiah Sundareshan Shrikrishna Isloor Priya Gupta Pagala Jasmeen Madhuranjana Gargi Sathi Mallick Nagendra R. Hegde
author_sort	Madhavi Annamanedi
title	Molecular fingerprinting of bovine mastitis-associated Staphylococcus aureus isolates from India
title_short	Molecular fingerprinting of bovine mastitis-associated Staphylococcus aureus isolates from India
title_full	Molecular fingerprinting of bovine mastitis-associated Staphylococcus aureus isolates from India
title_fullStr	Molecular fingerprinting of bovine mastitis-associated Staphylococcus aureus isolates from India
title_full_unstemmed	Molecular fingerprinting of bovine mastitis-associated Staphylococcus aureus isolates from India
title_sort	molecular fingerprinting of bovine mastitis-associated staphylococcus aureus isolates from india
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a5611aac6ced45c58f5b7f33dc3aa8cf
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Molecular fingerprinting of bovine mastitis-associated Staphylococcus aureus isolates from India

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