Effects of camptothecin on histological structures and gene expression profiles of fat bodies in Spodoptera frugiperda

Spodoptera frugiperda is a serious threat to global food production. Our previous study demonstrated that Camptothecin (CPT), a bioactive secondary metabolite from Camptotheca acuminata (Decne: Nyssaceae), exhibits adverse impact on the larval midgut of S. frugiperda and inhibits insect growth. Howe...

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Autores principales:	Benshui Shu, Xianmei Yang, Jinghua Dai, Haikuo Yu, Jingcheng Yu, Xiangli Li, Liang Cao, Jintian Lin
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Spodoptera frugiperda Camptothecin Fat body Histological changes Transcriptome analysis Environmental pollution TD172-193.5 Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/a565b0669a864d06b089c689d4893074
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spelling	oai:doaj.org-article:a565b0669a864d06b089c689d48930742021-11-10T04:17:20ZEffects of camptothecin on histological structures and gene expression profiles of fat bodies in Spodoptera frugiperda0147-651310.1016/j.ecoenv.2021.112968https://doaj.org/article/a565b0669a864d06b089c689d48930742021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0147651321010800https://doaj.org/toc/0147-6513Spodoptera frugiperda is a serious threat to global food production. Our previous study demonstrated that Camptothecin (CPT), a bioactive secondary metabolite from Camptotheca acuminata (Decne: Nyssaceae), exhibits adverse impact on the larval midgut of S. frugiperda and inhibits insect growth. However, effects of CPT on fat bodies of S. frugiperda larvae have not been examined yet. In the present study, we found that histological structures of fat bodies of S. frugiperda larvae were damaged in insects treated with CPT. Comparative transcriptomic analyses among different fat body samples from controls and insects treated with 1.0 and 5.0 μg/g CPT were performed. A total of 4212 and 5044 differentially expressed genes (DEGs) were identified in the samples treated with 1.0 and 5.0 μg/g CPT, respectively. Our data indicated that the pathways of detoxification, immune response, fatty acids, chitin, and hormone biosynthesis in fat bodies were affected by CPT treatments based on DEGs. These results provided a comprehensive view of the damage and gene expression changes in fat bodies of S. frugiperda after CPT exposure, which shall be useful to reveal the mechanism of CPT toxicity against S. frugiperda in future.Benshui ShuXianmei YangJinghua DaiHaikuo YuJingcheng YuXiangli LiLiang CaoJintian LinElsevierarticleSpodoptera frugiperdaCamptothecinFat bodyHistological changesTranscriptome analysisEnvironmental pollutionTD172-193.5Environmental sciencesGE1-350ENEcotoxicology and Environmental Safety, Vol 228, Iss , Pp 112968- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Spodoptera frugiperda Camptothecin Fat body Histological changes Transcriptome analysis Environmental pollution TD172-193.5 Environmental sciences GE1-350
spellingShingle	Spodoptera frugiperda Camptothecin Fat body Histological changes Transcriptome analysis Environmental pollution TD172-193.5 Environmental sciences GE1-350 Benshui Shu Xianmei Yang Jinghua Dai Haikuo Yu Jingcheng Yu Xiangli Li Liang Cao Jintian Lin Effects of camptothecin on histological structures and gene expression profiles of fat bodies in Spodoptera frugiperda
description	Spodoptera frugiperda is a serious threat to global food production. Our previous study demonstrated that Camptothecin (CPT), a bioactive secondary metabolite from Camptotheca acuminata (Decne: Nyssaceae), exhibits adverse impact on the larval midgut of S. frugiperda and inhibits insect growth. However, effects of CPT on fat bodies of S. frugiperda larvae have not been examined yet. In the present study, we found that histological structures of fat bodies of S. frugiperda larvae were damaged in insects treated with CPT. Comparative transcriptomic analyses among different fat body samples from controls and insects treated with 1.0 and 5.0 μg/g CPT were performed. A total of 4212 and 5044 differentially expressed genes (DEGs) were identified in the samples treated with 1.0 and 5.0 μg/g CPT, respectively. Our data indicated that the pathways of detoxification, immune response, fatty acids, chitin, and hormone biosynthesis in fat bodies were affected by CPT treatments based on DEGs. These results provided a comprehensive view of the damage and gene expression changes in fat bodies of S. frugiperda after CPT exposure, which shall be useful to reveal the mechanism of CPT toxicity against S. frugiperda in future.
format	article
author	Benshui Shu Xianmei Yang Jinghua Dai Haikuo Yu Jingcheng Yu Xiangli Li Liang Cao Jintian Lin
author_facet	Benshui Shu Xianmei Yang Jinghua Dai Haikuo Yu Jingcheng Yu Xiangli Li Liang Cao Jintian Lin
author_sort	Benshui Shu
title	Effects of camptothecin on histological structures and gene expression profiles of fat bodies in Spodoptera frugiperda
title_short	Effects of camptothecin on histological structures and gene expression profiles of fat bodies in Spodoptera frugiperda
title_full	Effects of camptothecin on histological structures and gene expression profiles of fat bodies in Spodoptera frugiperda
title_fullStr	Effects of camptothecin on histological structures and gene expression profiles of fat bodies in Spodoptera frugiperda
title_full_unstemmed	Effects of camptothecin on histological structures and gene expression profiles of fat bodies in Spodoptera frugiperda
title_sort	effects of camptothecin on histological structures and gene expression profiles of fat bodies in spodoptera frugiperda
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/a565b0669a864d06b089c689d4893074
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Effects of camptothecin on histological structures and gene expression profiles of fat bodies in Spodoptera frugiperda

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