Distinct roles of Plasmodium rhomboid 1 in parasite development and malaria pathogenesis.

Invasion of host cells by the malaria parasite involves recognition and interaction with cell-surface receptors. A wide variety of parasite surface proteins participate in this process, most of which are specific to the parasite's particular invasive form. Upon entry, the parasite has to dissoc...

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Autores principales:	Prakash Srinivasan, Isabelle Coppens, Marcelo Jacobs-Lorena
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a567e82e10de4029ac513051b88ac444
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spelling	oai:doaj.org-article:a567e82e10de4029ac513051b88ac4442021-11-25T05:47:21ZDistinct roles of Plasmodium rhomboid 1 in parasite development and malaria pathogenesis.1553-73661553-737410.1371/journal.ppat.1000262https://doaj.org/article/a567e82e10de4029ac513051b88ac4442009-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19148267/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Invasion of host cells by the malaria parasite involves recognition and interaction with cell-surface receptors. A wide variety of parasite surface proteins participate in this process, most of which are specific to the parasite's particular invasive form. Upon entry, the parasite has to dissociate itself from the host-cell receptors. One mechanism by which it does so is by shedding its surface ligands using specific enzymes. Rhomboid belongs to a family of serine proteases that cleave cell-surface proteins within their transmembrane domains. Here we identify and partially characterize a Plasmodium berghei rhomboid protease (PbROM1) that plays distinct roles during parasite development. PbROM1 localizes to the surface of sporozoites after salivary gland invasion. In blood stage merozoites, PbROM1 localizes to the apical end where proteins involved in invasion are also present. Our genetic analysis suggests that PbROM1 functions in the invasive stages of parasite development. Whereas wild-type P. berghei is lethal to mice, animals infected with PbROM1 null mutants clear the parasites efficiently and develop long-lasting protective immunity. The results indicate that P. berghei Rhomboid 1 plays a nonessential but important role during parasite development and identify rhomboid proteases as potential targets for disease control.Prakash SrinivasanIsabelle CoppensMarcelo Jacobs-LorenaPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 5, Iss 1, p e1000262 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Prakash Srinivasan Isabelle Coppens Marcelo Jacobs-Lorena Distinct roles of Plasmodium rhomboid 1 in parasite development and malaria pathogenesis.
description	Invasion of host cells by the malaria parasite involves recognition and interaction with cell-surface receptors. A wide variety of parasite surface proteins participate in this process, most of which are specific to the parasite's particular invasive form. Upon entry, the parasite has to dissociate itself from the host-cell receptors. One mechanism by which it does so is by shedding its surface ligands using specific enzymes. Rhomboid belongs to a family of serine proteases that cleave cell-surface proteins within their transmembrane domains. Here we identify and partially characterize a Plasmodium berghei rhomboid protease (PbROM1) that plays distinct roles during parasite development. PbROM1 localizes to the surface of sporozoites after salivary gland invasion. In blood stage merozoites, PbROM1 localizes to the apical end where proteins involved in invasion are also present. Our genetic analysis suggests that PbROM1 functions in the invasive stages of parasite development. Whereas wild-type P. berghei is lethal to mice, animals infected with PbROM1 null mutants clear the parasites efficiently and develop long-lasting protective immunity. The results indicate that P. berghei Rhomboid 1 plays a nonessential but important role during parasite development and identify rhomboid proteases as potential targets for disease control.
format	article
author	Prakash Srinivasan Isabelle Coppens Marcelo Jacobs-Lorena
author_facet	Prakash Srinivasan Isabelle Coppens Marcelo Jacobs-Lorena
author_sort	Prakash Srinivasan
title	Distinct roles of Plasmodium rhomboid 1 in parasite development and malaria pathogenesis.
title_short	Distinct roles of Plasmodium rhomboid 1 in parasite development and malaria pathogenesis.
title_full	Distinct roles of Plasmodium rhomboid 1 in parasite development and malaria pathogenesis.
title_fullStr	Distinct roles of Plasmodium rhomboid 1 in parasite development and malaria pathogenesis.
title_full_unstemmed	Distinct roles of Plasmodium rhomboid 1 in parasite development and malaria pathogenesis.
title_sort	distinct roles of plasmodium rhomboid 1 in parasite development and malaria pathogenesis.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/a567e82e10de4029ac513051b88ac444
work_keys_str_mv	AT prakashsrinivasan distinctrolesofplasmodiumrhomboid1inparasitedevelopmentandmalariapathogenesis AT isabellecoppens distinctrolesofplasmodiumrhomboid1inparasitedevelopmentandmalariapathogenesis AT marcelojacobslorena distinctrolesofplasmodiumrhomboid1inparasitedevelopmentandmalariapathogenesis
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Distinct roles of Plasmodium rhomboid 1 in parasite development and malaria pathogenesis.

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