A metabolomics approach to evaluate the effect of lyophilization versus oven drying on the chemical composition of plant extracts

Abstract Lyophilization is the “gold standard” for drying plant extracts, which is important in preserving their quality and extending their shelf-life. Compared to other methods of drying plant extracts, lyophilization is costlier due to equipment, material and operational expenses. An alternative...

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Autores principales:	Nancy A. ElNaker, Mariane Daou, Michael A. Ochsenkühn, Shady A. Amin, Ahmed F. Yousef, Lina F. Yousef
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a56bf31222f142f08091faadcd42f4ae
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spelling	oai:doaj.org-article:a56bf31222f142f08091faadcd42f4ae2021-11-28T12:15:30ZA metabolomics approach to evaluate the effect of lyophilization versus oven drying on the chemical composition of plant extracts10.1038/s41598-021-02158-62045-2322https://doaj.org/article/a56bf31222f142f08091faadcd42f4ae2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02158-6https://doaj.org/toc/2045-2322Abstract Lyophilization is the “gold standard” for drying plant extracts, which is important in preserving their quality and extending their shelf-life. Compared to other methods of drying plant extracts, lyophilization is costlier due to equipment, material and operational expenses. An alternative method is post-extraction oven-drying, but the effects of this process on extract quality are unknown. In this study, crude extracts from Arthrocnemum macrostachyum shoots were compared using three post-extraction drying methods (lyophilization and oven drying at 40 and 60 °C) and two extraction solvents (water and aqueous 50% ethanol). Untargeted metabolomics coupled with chemometrics analysis revealed that post extraction oven-drying resulted in the loss of up to 27% of molecular features when compared to lyophilization in water extracts only. In contrast, only 3% of molecular features were lost in aqueous 50% ethanol extracts when subjected to oven drying. That is to say, ethanol used as a solvent has a stabilizing effect on metabolites and enhances their resistance to thermal transformation in the oven. Collectively, oven-drying of extracts was as effective as lyophilization in preserving metabolites in extracts only when 50% ethanol was used as a solvent. The results presented in this paper demonstrate the value of selecting solvent-appropriate post-extraction drying methods.Nancy A. ElNakerMariane DaouMichael A. OchsenkühnShady A. AminAhmed F. YousefLina F. YousefNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nancy A. ElNaker Mariane Daou Michael A. Ochsenkühn Shady A. Amin Ahmed F. Yousef Lina F. Yousef A metabolomics approach to evaluate the effect of lyophilization versus oven drying on the chemical composition of plant extracts
description	Abstract Lyophilization is the “gold standard” for drying plant extracts, which is important in preserving their quality and extending their shelf-life. Compared to other methods of drying plant extracts, lyophilization is costlier due to equipment, material and operational expenses. An alternative method is post-extraction oven-drying, but the effects of this process on extract quality are unknown. In this study, crude extracts from Arthrocnemum macrostachyum shoots were compared using three post-extraction drying methods (lyophilization and oven drying at 40 and 60 °C) and two extraction solvents (water and aqueous 50% ethanol). Untargeted metabolomics coupled with chemometrics analysis revealed that post extraction oven-drying resulted in the loss of up to 27% of molecular features when compared to lyophilization in water extracts only. In contrast, only 3% of molecular features were lost in aqueous 50% ethanol extracts when subjected to oven drying. That is to say, ethanol used as a solvent has a stabilizing effect on metabolites and enhances their resistance to thermal transformation in the oven. Collectively, oven-drying of extracts was as effective as lyophilization in preserving metabolites in extracts only when 50% ethanol was used as a solvent. The results presented in this paper demonstrate the value of selecting solvent-appropriate post-extraction drying methods.
format	article
author	Nancy A. ElNaker Mariane Daou Michael A. Ochsenkühn Shady A. Amin Ahmed F. Yousef Lina F. Yousef
author_facet	Nancy A. ElNaker Mariane Daou Michael A. Ochsenkühn Shady A. Amin Ahmed F. Yousef Lina F. Yousef
author_sort	Nancy A. ElNaker
title	A metabolomics approach to evaluate the effect of lyophilization versus oven drying on the chemical composition of plant extracts
title_short	A metabolomics approach to evaluate the effect of lyophilization versus oven drying on the chemical composition of plant extracts
title_full	A metabolomics approach to evaluate the effect of lyophilization versus oven drying on the chemical composition of plant extracts
title_fullStr	A metabolomics approach to evaluate the effect of lyophilization versus oven drying on the chemical composition of plant extracts
title_full_unstemmed	A metabolomics approach to evaluate the effect of lyophilization versus oven drying on the chemical composition of plant extracts
title_sort	metabolomics approach to evaluate the effect of lyophilization versus oven drying on the chemical composition of plant extracts
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a56bf31222f142f08091faadcd42f4ae
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A metabolomics approach to evaluate the effect of lyophilization versus oven drying on the chemical composition of plant extracts

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