Insights into Regulation of C<sub>2</sub> and C<sub>4</sub> Photosynthesis in <i>Amaranthaceae</i>/<i>Chenopodiaceae</i> Using RNA-Seq
<i>Amaranthaceae</i> (incl. <i>Chenopodiaceae</i>) shows an immense diversity of C<sub>4</sub> syndromes. More than 15 independent origins of C<sub>4</sub> photosynthesis, and the largest number of C<sub>4</sub> species in eudicots signify...
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| Autores principales: | , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/a56cb2b3f0994006ab4776913b38c55e |
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| Sumario: | <i>Amaranthaceae</i> (incl. <i>Chenopodiaceae</i>) shows an immense diversity of C<sub>4</sub> syndromes. More than 15 independent origins of C<sub>4</sub> photosynthesis, and the largest number of C<sub>4</sub> species in eudicots signify the importance of this angiosperm lineage in C<sub>4</sub> evolution. Here, we conduct RNA-Seq followed by comparative transcriptome analysis of three species from <i>Camphorosmeae</i> representing related clades with different photosynthetic types: <i>Threlkeldia diffusa</i> (C<sub>3</sub>), <i>Sedobassia sedoides</i> (C<sub>2</sub>), and <i>Bassia prostrata</i> (C<sub>4</sub>). Results show that <i>B. prostrata</i> belongs to the NADP-ME type and core genes encoding for C<sub>4</sub> cycle are significantly upregulated when compared with <i>Sed. sedoides</i> and <i>T. diffusa</i>. <i>Sedobassia sedoides</i> and <i>B. prostrata</i> share a number of upregulated C<sub>4</sub>-related genes; however, two C<sub>4</sub> transporters (DIT and TPT) are found significantly upregulated only in <i>Sed. sedoides</i>. Combined analysis of transcription factors (TFs) of the closely related lineages (<i>Camphorosmeae</i> and <i>Salsoleae</i>) revealed that no C<sub>3</sub>-specific TFs are higher in C<sub>2</sub> species compared with C<sub>4</sub> species; instead, the C<sub>2</sub> species show their own set of upregulated TFs. Taken together, our study indicates that the hypothesis of the C<sub>2</sub> photosynthesis as a proxy towards C<sub>4</sub> photosynthesis is questionable in <i>Sed. sedoides</i> and more in favour of an independent evolutionary stable state. |
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