Progesterone, Myo-Inositol, Dopamine and Prolactin Present in Follicular Fluid Have Differential Effects on Sperm Motility Subpopulations

Considering the challenges surrounding causative factors in male infertility, rather than relying on standard semen analysis, the assessment of sperm subpopulations and functional characteristics essential for fertilization is paramount. Furthermore, the diagnostic value of sperm interactions with b...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shannen Keyser, Gerhard van der Horst, Liana Maree
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	progesterone myo-inositol dopamine prolactin sperm subpopulations functional characteristics Science Q
Acceso en línea:	https://doaj.org/article/a56e9701c7674fdab65e6ae6d657d9d2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a56e9701c7674fdab65e6ae6d657d9d2
record_format	dspace
spelling	oai:doaj.org-article:a56e9701c7674fdab65e6ae6d657d9d22021-11-25T18:11:34ZProgesterone, Myo-Inositol, Dopamine and Prolactin Present in Follicular Fluid Have Differential Effects on Sperm Motility Subpopulations10.3390/life111112502075-1729https://doaj.org/article/a56e9701c7674fdab65e6ae6d657d9d22021-11-01T00:00:00Zhttps://www.mdpi.com/2075-1729/11/11/1250https://doaj.org/toc/2075-1729Considering the challenges surrounding causative factors in male infertility, rather than relying on standard semen analysis, the assessment of sperm subpopulations and functional characteristics essential for fertilization is paramount. Furthermore, the diagnostic value of sperm interactions with biological components in the female reproductive tract may improve our understanding of subfertility and provide applications in assisted reproductive techniques. We investigated the response of two sperm motility subpopulations (mimicking the functionality of potentially fertile and sub-fertile semen samples) to biological substances present in the female reproductive tract. Donor semen was separated via double density gradient centrifugation, isolated into high (HM) and low motile (LM) sperm subpopulations and incubated in human tubal fluid (HTF), capacitating HTF, HD-C medium, progesterone, myo-inositol, dopamine and prolactin. Treated subpopulations were evaluated for vitality, motility percentages and kinematic parameters, hyperactivation, positive reactive oxygen species (ROS), intact mitochondrial membrane potential (MMP) and acrosome reaction (AR). While all media had a significantly positive effect on the LM subpopulation, dopamine appeared to significantly improve both subpopulations’ functional characteristics. HD-C, progesterone and myo-inositol resulted in increased motility, kinematic and hyperactivation parameters, whereas prolactin and myo-inositol improved the LM subpopulations’ MMP intactness and reduced ROS. Furthermore, progesterone, myo-inositol and dopamine improved the HM subpopulations’ motility parameters and AR. Our results suggest that treatment of sub-fertile semen samples with biological substances present in follicular fluid might assist the development of new strategies for IVF treatment.Shannen KeyserGerhard van der HorstLiana MareeMDPI AGarticleprogesteronemyo-inositoldopamineprolactinsperm subpopulationsfunctional characteristicsScienceQENLife, Vol 11, Iss 1250, p 1250 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	progesterone myo-inositol dopamine prolactin sperm subpopulations functional characteristics Science Q
spellingShingle	progesterone myo-inositol dopamine prolactin sperm subpopulations functional characteristics Science Q Shannen Keyser Gerhard van der Horst Liana Maree Progesterone, Myo-Inositol, Dopamine and Prolactin Present in Follicular Fluid Have Differential Effects on Sperm Motility Subpopulations
description	Considering the challenges surrounding causative factors in male infertility, rather than relying on standard semen analysis, the assessment of sperm subpopulations and functional characteristics essential for fertilization is paramount. Furthermore, the diagnostic value of sperm interactions with biological components in the female reproductive tract may improve our understanding of subfertility and provide applications in assisted reproductive techniques. We investigated the response of two sperm motility subpopulations (mimicking the functionality of potentially fertile and sub-fertile semen samples) to biological substances present in the female reproductive tract. Donor semen was separated via double density gradient centrifugation, isolated into high (HM) and low motile (LM) sperm subpopulations and incubated in human tubal fluid (HTF), capacitating HTF, HD-C medium, progesterone, myo-inositol, dopamine and prolactin. Treated subpopulations were evaluated for vitality, motility percentages and kinematic parameters, hyperactivation, positive reactive oxygen species (ROS), intact mitochondrial membrane potential (MMP) and acrosome reaction (AR). While all media had a significantly positive effect on the LM subpopulation, dopamine appeared to significantly improve both subpopulations’ functional characteristics. HD-C, progesterone and myo-inositol resulted in increased motility, kinematic and hyperactivation parameters, whereas prolactin and myo-inositol improved the LM subpopulations’ MMP intactness and reduced ROS. Furthermore, progesterone, myo-inositol and dopamine improved the HM subpopulations’ motility parameters and AR. Our results suggest that treatment of sub-fertile semen samples with biological substances present in follicular fluid might assist the development of new strategies for IVF treatment.
format	article
author	Shannen Keyser Gerhard van der Horst Liana Maree
author_facet	Shannen Keyser Gerhard van der Horst Liana Maree
author_sort	Shannen Keyser
title	Progesterone, Myo-Inositol, Dopamine and Prolactin Present in Follicular Fluid Have Differential Effects on Sperm Motility Subpopulations
title_short	Progesterone, Myo-Inositol, Dopamine and Prolactin Present in Follicular Fluid Have Differential Effects on Sperm Motility Subpopulations
title_full	Progesterone, Myo-Inositol, Dopamine and Prolactin Present in Follicular Fluid Have Differential Effects on Sperm Motility Subpopulations
title_fullStr	Progesterone, Myo-Inositol, Dopamine and Prolactin Present in Follicular Fluid Have Differential Effects on Sperm Motility Subpopulations
title_full_unstemmed	Progesterone, Myo-Inositol, Dopamine and Prolactin Present in Follicular Fluid Have Differential Effects on Sperm Motility Subpopulations
title_sort	progesterone, myo-inositol, dopamine and prolactin present in follicular fluid have differential effects on sperm motility subpopulations
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a56e9701c7674fdab65e6ae6d657d9d2
work_keys_str_mv	AT shannenkeyser progesteronemyoinositoldopamineandprolactinpresentinfollicularfluidhavedifferentialeffectsonspermmotilitysubpopulations AT gerhardvanderhorst progesteronemyoinositoldopamineandprolactinpresentinfollicularfluidhavedifferentialeffectsonspermmotilitysubpopulations AT lianamaree progesteronemyoinositoldopamineandprolactinpresentinfollicularfluidhavedifferentialeffectsonspermmotilitysubpopulations
_version_	1718411515766243328

Progesterone, Myo-Inositol, Dopamine and Prolactin Present in Follicular Fluid Have Differential Effects on Sperm Motility Subpopulations

Ejemplares similares