Enteric innervation combined with proteomics for the evaluation of the effects of chronic fluoride exposure on the duodenum of rats

Abstract Ingested fluoride (F) is absorbed mainly in the small intestine, which is controlled by the Enteric Nervous System (ENS). Although important intestinal symptomatology has been described after excessive F exposure, there have been no studies reporting the effects of F on the ENS. In this stu...

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Autores principales:	Carina Guimarães de Souza Melo, Juliana Vanessa Colombo Martins Perles, Jacqueline Nelisis Zanoni, Sara Raquel Garcia de Souza, Erika Xavier Santos, Aline de Lima Leite, Alessandro Domingues Heubel, Camila Oliveira e Souza, Juliana Gadelha de Souza, Marília Afonso Rabelo Buzalaf
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a57045d295f74bb68c18ba90c1c5c1d8
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spelling	oai:doaj.org-article:a57045d295f74bb68c18ba90c1c5c1d82021-12-02T11:52:34ZEnteric innervation combined with proteomics for the evaluation of the effects of chronic fluoride exposure on the duodenum of rats10.1038/s41598-017-01090-y2045-2322https://doaj.org/article/a57045d295f74bb68c18ba90c1c5c1d82017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01090-yhttps://doaj.org/toc/2045-2322Abstract Ingested fluoride (F) is absorbed mainly in the small intestine, which is controlled by the Enteric Nervous System (ENS). Although important intestinal symptomatology has been described after excessive F exposure, there have been no studies reporting the effects of F on the ENS. In this study, the effects of chronic F exposure were evaluated on the duodenums of rats through proteomic and morphological analyses. Concentrations of 0, 10, or 50 ppm of F were applied to the drinking water for 30 days. Immunofluorescence techniques were performed in the myenteric plexus of the duodenum to detect HuC/D, neuronal nitric oxide (nNOS), vasoactive intestinal peptide (VIP), calcitonin gene related peptide (CGRP), and substance P (SP). The 50 ppm F group presented a significant decrease in the density of nNOS-IR neurons. Significant morphological alterations were also observed in HUC/D-IR and nNOS-IR neurons; VIP-IR, CGRP-IR, and SP-IR varicosities for both groups (10 and 50 ppm F). Proteomic analysis of the duodenum demonstrated alterations in the expression of several proteins, especially those related to important biological processes, such as protein polymerization, which helps to explain the downregulation of many proteins upon exposure to 50 ppm of F.Carina Guimarães de Souza MeloJuliana Vanessa Colombo Martins PerlesJacqueline Nelisis ZanoniSara Raquel Garcia de SouzaErika Xavier SantosAline de Lima LeiteAlessandro Domingues HeubelCamila Oliveira e SouzaJuliana Gadelha de SouzaMarília Afonso Rabelo BuzalafNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Carina Guimarães de Souza Melo Juliana Vanessa Colombo Martins Perles Jacqueline Nelisis Zanoni Sara Raquel Garcia de Souza Erika Xavier Santos Aline de Lima Leite Alessandro Domingues Heubel Camila Oliveira e Souza Juliana Gadelha de Souza Marília Afonso Rabelo Buzalaf Enteric innervation combined with proteomics for the evaluation of the effects of chronic fluoride exposure on the duodenum of rats
description	Abstract Ingested fluoride (F) is absorbed mainly in the small intestine, which is controlled by the Enteric Nervous System (ENS). Although important intestinal symptomatology has been described after excessive F exposure, there have been no studies reporting the effects of F on the ENS. In this study, the effects of chronic F exposure were evaluated on the duodenums of rats through proteomic and morphological analyses. Concentrations of 0, 10, or 50 ppm of F were applied to the drinking water for 30 days. Immunofluorescence techniques were performed in the myenteric plexus of the duodenum to detect HuC/D, neuronal nitric oxide (nNOS), vasoactive intestinal peptide (VIP), calcitonin gene related peptide (CGRP), and substance P (SP). The 50 ppm F group presented a significant decrease in the density of nNOS-IR neurons. Significant morphological alterations were also observed in HUC/D-IR and nNOS-IR neurons; VIP-IR, CGRP-IR, and SP-IR varicosities for both groups (10 and 50 ppm F). Proteomic analysis of the duodenum demonstrated alterations in the expression of several proteins, especially those related to important biological processes, such as protein polymerization, which helps to explain the downregulation of many proteins upon exposure to 50 ppm of F.
format	article
author	Carina Guimarães de Souza Melo Juliana Vanessa Colombo Martins Perles Jacqueline Nelisis Zanoni Sara Raquel Garcia de Souza Erika Xavier Santos Aline de Lima Leite Alessandro Domingues Heubel Camila Oliveira e Souza Juliana Gadelha de Souza Marília Afonso Rabelo Buzalaf
author_facet	Carina Guimarães de Souza Melo Juliana Vanessa Colombo Martins Perles Jacqueline Nelisis Zanoni Sara Raquel Garcia de Souza Erika Xavier Santos Aline de Lima Leite Alessandro Domingues Heubel Camila Oliveira e Souza Juliana Gadelha de Souza Marília Afonso Rabelo Buzalaf
author_sort	Carina Guimarães de Souza Melo
title	Enteric innervation combined with proteomics for the evaluation of the effects of chronic fluoride exposure on the duodenum of rats
title_short	Enteric innervation combined with proteomics for the evaluation of the effects of chronic fluoride exposure on the duodenum of rats
title_full	Enteric innervation combined with proteomics for the evaluation of the effects of chronic fluoride exposure on the duodenum of rats
title_fullStr	Enteric innervation combined with proteomics for the evaluation of the effects of chronic fluoride exposure on the duodenum of rats
title_full_unstemmed	Enteric innervation combined with proteomics for the evaluation of the effects of chronic fluoride exposure on the duodenum of rats
title_sort	enteric innervation combined with proteomics for the evaluation of the effects of chronic fluoride exposure on the duodenum of rats
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a57045d295f74bb68c18ba90c1c5c1d8
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Enteric innervation combined with proteomics for the evaluation of the effects of chronic fluoride exposure on the duodenum of rats

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