Magnetic responsive hydroxyapatite composite scaffolds construction for bone defect reparation

Xiao Bo Zeng, Hao Hu, Li Qin Xie, Fang Lan, Wen Jiang, Yao Wu, Zhong Wei GuNational Engineering Research Center for Biomaterials, Sichuan University, Chengdu, Sichuan, People&#39;s Republic of ChinaIntroduction: In recent years, interest in magnetic biomimetic scaffolds for tissue engineerin...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zeng XB, Hu H, Xie LQ, Lan F, Jiang W, Wu Y, Gu ZW
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a57706202c8b473e8044f4c5530b569b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a57706202c8b473e8044f4c5530b569b
record_format	dspace
spelling	oai:doaj.org-article:a57706202c8b473e8044f4c5530b569b2021-12-02T06:16:08ZMagnetic responsive hydroxyapatite composite scaffolds construction for bone defect reparation1176-91141178-2013https://doaj.org/article/a57706202c8b473e8044f4c5530b569b2012-07-01T00:00:00Zhttp://www.dovepress.com/magnetic-responsive-hydroxyapatite-composite-scaffolds-construction-fo-a10298https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Xiao Bo Zeng, Hao Hu, Li Qin Xie, Fang Lan, Wen Jiang, Yao Wu, Zhong Wei GuNational Engineering Research Center for Biomaterials, Sichuan University, Chengdu, Sichuan, People&#39;s Republic of ChinaIntroduction: In recent years, interest in magnetic biomimetic scaffolds for tissue engineering has increased considerably. A type of magnetic scaffold composed of magnetic nanoparticles (MNPs) and hydroxyapatite (HA) for bone repair has been developed by our research group.Aim and methods: In this study, to investigate the influence of the MNP content (in the scaffolds) on the cell behaviors and the interactions between the magnetic scaffold and the exterior magnetic field, a series of MNP-HA magnetic scaffolds with different MNP contents (from 0.2% to 2%) were fabricated by immersing HA scaffold into MNP colloid. ROS 17/2.8 and MC3T3-E1 cells were cultured on the scaffolds in vitro, with and without an exterior magnetic field, respectively. The cell adhesion, proliferation and differentiation were evaluated via scanning electron microscopy; confocal laser scanning microscopy; and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), alkaline phosphatase, and bone gla protein activity tests.Results: The results demonstrated the positive influence of the magnetic scaffolds on cell adhesion, proliferation, and differentiation. Further, a higher amount of MNPs on the magnetic scaffolds led to more significant stimulation.Conclusion: The magnetic scaffold can respond to the exterior magnetic field and engender some synergistic effect to intensify the stimulating effect of a magnetic field to the proliferation and differentiation of cells.Keywords: magnetic therapy, magnetic nanoparticles, bone repair, magnetic responsiveZeng XBHu HXie LQLan FJiang WWu YGu ZWDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 3365-3378 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Zeng XB Hu H Xie LQ Lan F Jiang W Wu Y Gu ZW Magnetic responsive hydroxyapatite composite scaffolds construction for bone defect reparation
description	Xiao Bo Zeng, Hao Hu, Li Qin Xie, Fang Lan, Wen Jiang, Yao Wu, Zhong Wei GuNational Engineering Research Center for Biomaterials, Sichuan University, Chengdu, Sichuan, People&#39;s Republic of ChinaIntroduction: In recent years, interest in magnetic biomimetic scaffolds for tissue engineering has increased considerably. A type of magnetic scaffold composed of magnetic nanoparticles (MNPs) and hydroxyapatite (HA) for bone repair has been developed by our research group.Aim and methods: In this study, to investigate the influence of the MNP content (in the scaffolds) on the cell behaviors and the interactions between the magnetic scaffold and the exterior magnetic field, a series of MNP-HA magnetic scaffolds with different MNP contents (from 0.2% to 2%) were fabricated by immersing HA scaffold into MNP colloid. ROS 17/2.8 and MC3T3-E1 cells were cultured on the scaffolds in vitro, with and without an exterior magnetic field, respectively. The cell adhesion, proliferation and differentiation were evaluated via scanning electron microscopy; confocal laser scanning microscopy; and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), alkaline phosphatase, and bone gla protein activity tests.Results: The results demonstrated the positive influence of the magnetic scaffolds on cell adhesion, proliferation, and differentiation. Further, a higher amount of MNPs on the magnetic scaffolds led to more significant stimulation.Conclusion: The magnetic scaffold can respond to the exterior magnetic field and engender some synergistic effect to intensify the stimulating effect of a magnetic field to the proliferation and differentiation of cells.Keywords: magnetic therapy, magnetic nanoparticles, bone repair, magnetic responsive
format	article
author	Zeng XB Hu H Xie LQ Lan F Jiang W Wu Y Gu ZW
author_facet	Zeng XB Hu H Xie LQ Lan F Jiang W Wu Y Gu ZW
author_sort	Zeng XB
title	Magnetic responsive hydroxyapatite composite scaffolds construction for bone defect reparation
title_short	Magnetic responsive hydroxyapatite composite scaffolds construction for bone defect reparation
title_full	Magnetic responsive hydroxyapatite composite scaffolds construction for bone defect reparation
title_fullStr	Magnetic responsive hydroxyapatite composite scaffolds construction for bone defect reparation
title_full_unstemmed	Magnetic responsive hydroxyapatite composite scaffolds construction for bone defect reparation
title_sort	magnetic responsive hydroxyapatite composite scaffolds construction for bone defect reparation
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/a57706202c8b473e8044f4c5530b569b
work_keys_str_mv	AT zengxb magneticresponsivehydroxyapatitecompositescaffoldsconstructionforbonedefectreparation AT huh magneticresponsivehydroxyapatitecompositescaffoldsconstructionforbonedefectreparation AT xielq magneticresponsivehydroxyapatitecompositescaffoldsconstructionforbonedefectreparation AT lanf magneticresponsivehydroxyapatitecompositescaffoldsconstructionforbonedefectreparation AT jiangw magneticresponsivehydroxyapatitecompositescaffoldsconstructionforbonedefectreparation AT wuy magneticresponsivehydroxyapatitecompositescaffoldsconstructionforbonedefectreparation AT guzw magneticresponsivehydroxyapatitecompositescaffoldsconstructionforbonedefectreparation
_version_	1718400006984040448

Magnetic responsive hydroxyapatite composite scaffolds construction for bone defect reparation

Ejemplares similares