Dual-delivery of FGF-2/CTGF from Silk Fibroin/PLCL-PEO Coaxial Fibers Enhances MSC Proliferation and Fibrogenesis

Abstract The success of mesenchymal stem cell transplantation is highly dependent on their survival and controlled fate regulation. This study demonstrates that dual-delivery of connective tissue growth factor (CTGF) and fibroblast growth factor 2 (FGF-2) from a core-shell fiber of Silk Fibroin/poly...

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Autores principales: Ruodan Xu, Huiling Zhao, Hanif Muhammad, Mingdong Dong, Flemming Besenbacher, Menglin Chen
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Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/a57dae93087648a58136e6abf9a3f010
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spelling oai:doaj.org-article:a57dae93087648a58136e6abf9a3f0102021-12-02T16:06:45ZDual-delivery of FGF-2/CTGF from Silk Fibroin/PLCL-PEO Coaxial Fibers Enhances MSC Proliferation and Fibrogenesis10.1038/s41598-017-08226-02045-2322https://doaj.org/article/a57dae93087648a58136e6abf9a3f0102017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08226-0https://doaj.org/toc/2045-2322Abstract The success of mesenchymal stem cell transplantation is highly dependent on their survival and controlled fate regulation. This study demonstrates that dual-delivery of connective tissue growth factor (CTGF) and fibroblast growth factor 2 (FGF-2) from a core-shell fiber of Silk Fibroin/poly(L-lactic acid-co-ε-caprolactone)-polyethylene oxide (SF/PLCL-PEO) enhanced fibrogenic lineage differentiation of MSCs. The core-shell structure was confirmed by transmission electron microscopy (TEM), fluorescence microscopy and attenuated total reflection (ATR) Fourier transform infrared (FTIR) spectroscopy. A sequential release of FGF-2 and CTGF was successfully achieved in this manner. FGF-2 plays an important role in stem cell proliferation and, meanwhile when accompanied with CTGF, has a slightly additive effect on fibrogenic differentiation of MSCs, whereas CTGF promotes fibrogenesis and alleviates osteogenesis, chondrogenesis and adipogenesis.Ruodan XuHuiling ZhaoHanif MuhammadMingdong DongFlemming BesenbacherMenglin ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Ruodan Xu
Huiling Zhao
Hanif Muhammad
Mingdong Dong
Flemming Besenbacher
Menglin Chen
Dual-delivery of FGF-2/CTGF from Silk Fibroin/PLCL-PEO Coaxial Fibers Enhances MSC Proliferation and Fibrogenesis
description Abstract The success of mesenchymal stem cell transplantation is highly dependent on their survival and controlled fate regulation. This study demonstrates that dual-delivery of connective tissue growth factor (CTGF) and fibroblast growth factor 2 (FGF-2) from a core-shell fiber of Silk Fibroin/poly(L-lactic acid-co-ε-caprolactone)-polyethylene oxide (SF/PLCL-PEO) enhanced fibrogenic lineage differentiation of MSCs. The core-shell structure was confirmed by transmission electron microscopy (TEM), fluorescence microscopy and attenuated total reflection (ATR) Fourier transform infrared (FTIR) spectroscopy. A sequential release of FGF-2 and CTGF was successfully achieved in this manner. FGF-2 plays an important role in stem cell proliferation and, meanwhile when accompanied with CTGF, has a slightly additive effect on fibrogenic differentiation of MSCs, whereas CTGF promotes fibrogenesis and alleviates osteogenesis, chondrogenesis and adipogenesis.
format article
author Ruodan Xu
Huiling Zhao
Hanif Muhammad
Mingdong Dong
Flemming Besenbacher
Menglin Chen
author_facet Ruodan Xu
Huiling Zhao
Hanif Muhammad
Mingdong Dong
Flemming Besenbacher
Menglin Chen
author_sort Ruodan Xu
title Dual-delivery of FGF-2/CTGF from Silk Fibroin/PLCL-PEO Coaxial Fibers Enhances MSC Proliferation and Fibrogenesis
title_short Dual-delivery of FGF-2/CTGF from Silk Fibroin/PLCL-PEO Coaxial Fibers Enhances MSC Proliferation and Fibrogenesis
title_full Dual-delivery of FGF-2/CTGF from Silk Fibroin/PLCL-PEO Coaxial Fibers Enhances MSC Proliferation and Fibrogenesis
title_fullStr Dual-delivery of FGF-2/CTGF from Silk Fibroin/PLCL-PEO Coaxial Fibers Enhances MSC Proliferation and Fibrogenesis
title_full_unstemmed Dual-delivery of FGF-2/CTGF from Silk Fibroin/PLCL-PEO Coaxial Fibers Enhances MSC Proliferation and Fibrogenesis
title_sort dual-delivery of fgf-2/ctgf from silk fibroin/plcl-peo coaxial fibers enhances msc proliferation and fibrogenesis
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/a57dae93087648a58136e6abf9a3f010
work_keys_str_mv AT ruodanxu dualdeliveryoffgf2ctgffromsilkfibroinplclpeocoaxialfibersenhancesmscproliferationandfibrogenesis
AT huilingzhao dualdeliveryoffgf2ctgffromsilkfibroinplclpeocoaxialfibersenhancesmscproliferationandfibrogenesis
AT hanifmuhammad dualdeliveryoffgf2ctgffromsilkfibroinplclpeocoaxialfibersenhancesmscproliferationandfibrogenesis
AT mingdongdong dualdeliveryoffgf2ctgffromsilkfibroinplclpeocoaxialfibersenhancesmscproliferationandfibrogenesis
AT flemmingbesenbacher dualdeliveryoffgf2ctgffromsilkfibroinplclpeocoaxialfibersenhancesmscproliferationandfibrogenesis
AT menglinchen dualdeliveryoffgf2ctgffromsilkfibroinplclpeocoaxialfibersenhancesmscproliferationandfibrogenesis
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