Dual-delivery of FGF-2/CTGF from Silk Fibroin/PLCL-PEO Coaxial Fibers Enhances MSC Proliferation and Fibrogenesis

Abstract The success of mesenchymal stem cell transplantation is highly dependent on their survival and controlled fate regulation. This study demonstrates that dual-delivery of connective tissue growth factor (CTGF) and fibroblast growth factor 2 (FGF-2) from a core-shell fiber of Silk Fibroin/poly...

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Autores principales:	Ruodan Xu, Huiling Zhao, Hanif Muhammad, Mingdong Dong, Flemming Besenbacher, Menglin Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a57dae93087648a58136e6abf9a3f010
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spelling	oai:doaj.org-article:a57dae93087648a58136e6abf9a3f0102021-12-02T16:06:45ZDual-delivery of FGF-2/CTGF from Silk Fibroin/PLCL-PEO Coaxial Fibers Enhances MSC Proliferation and Fibrogenesis10.1038/s41598-017-08226-02045-2322https://doaj.org/article/a57dae93087648a58136e6abf9a3f0102017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08226-0https://doaj.org/toc/2045-2322Abstract The success of mesenchymal stem cell transplantation is highly dependent on their survival and controlled fate regulation. This study demonstrates that dual-delivery of connective tissue growth factor (CTGF) and fibroblast growth factor 2 (FGF-2) from a core-shell fiber of Silk Fibroin/poly(L-lactic acid-co-ε-caprolactone)-polyethylene oxide (SF/PLCL-PEO) enhanced fibrogenic lineage differentiation of MSCs. The core-shell structure was confirmed by transmission electron microscopy (TEM), fluorescence microscopy and attenuated total reflection (ATR) Fourier transform infrared (FTIR) spectroscopy. A sequential release of FGF-2 and CTGF was successfully achieved in this manner. FGF-2 plays an important role in stem cell proliferation and, meanwhile when accompanied with CTGF, has a slightly additive effect on fibrogenic differentiation of MSCs, whereas CTGF promotes fibrogenesis and alleviates osteogenesis, chondrogenesis and adipogenesis.Ruodan XuHuiling ZhaoHanif MuhammadMingdong DongFlemming BesenbacherMenglin ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ruodan Xu Huiling Zhao Hanif Muhammad Mingdong Dong Flemming Besenbacher Menglin Chen Dual-delivery of FGF-2/CTGF from Silk Fibroin/PLCL-PEO Coaxial Fibers Enhances MSC Proliferation and Fibrogenesis
description	Abstract The success of mesenchymal stem cell transplantation is highly dependent on their survival and controlled fate regulation. This study demonstrates that dual-delivery of connective tissue growth factor (CTGF) and fibroblast growth factor 2 (FGF-2) from a core-shell fiber of Silk Fibroin/poly(L-lactic acid-co-ε-caprolactone)-polyethylene oxide (SF/PLCL-PEO) enhanced fibrogenic lineage differentiation of MSCs. The core-shell structure was confirmed by transmission electron microscopy (TEM), fluorescence microscopy and attenuated total reflection (ATR) Fourier transform infrared (FTIR) spectroscopy. A sequential release of FGF-2 and CTGF was successfully achieved in this manner. FGF-2 plays an important role in stem cell proliferation and, meanwhile when accompanied with CTGF, has a slightly additive effect on fibrogenic differentiation of MSCs, whereas CTGF promotes fibrogenesis and alleviates osteogenesis, chondrogenesis and adipogenesis.
format	article
author	Ruodan Xu Huiling Zhao Hanif Muhammad Mingdong Dong Flemming Besenbacher Menglin Chen
author_facet	Ruodan Xu Huiling Zhao Hanif Muhammad Mingdong Dong Flemming Besenbacher Menglin Chen
author_sort	Ruodan Xu
title	Dual-delivery of FGF-2/CTGF from Silk Fibroin/PLCL-PEO Coaxial Fibers Enhances MSC Proliferation and Fibrogenesis
title_short	Dual-delivery of FGF-2/CTGF from Silk Fibroin/PLCL-PEO Coaxial Fibers Enhances MSC Proliferation and Fibrogenesis
title_full	Dual-delivery of FGF-2/CTGF from Silk Fibroin/PLCL-PEO Coaxial Fibers Enhances MSC Proliferation and Fibrogenesis
title_fullStr	Dual-delivery of FGF-2/CTGF from Silk Fibroin/PLCL-PEO Coaxial Fibers Enhances MSC Proliferation and Fibrogenesis
title_full_unstemmed	Dual-delivery of FGF-2/CTGF from Silk Fibroin/PLCL-PEO Coaxial Fibers Enhances MSC Proliferation and Fibrogenesis
title_sort	dual-delivery of fgf-2/ctgf from silk fibroin/plcl-peo coaxial fibers enhances msc proliferation and fibrogenesis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a57dae93087648a58136e6abf9a3f010
work_keys_str_mv	AT ruodanxu dualdeliveryoffgf2ctgffromsilkfibroinplclpeocoaxialfibersenhancesmscproliferationandfibrogenesis AT huilingzhao dualdeliveryoffgf2ctgffromsilkfibroinplclpeocoaxialfibersenhancesmscproliferationandfibrogenesis AT hanifmuhammad dualdeliveryoffgf2ctgffromsilkfibroinplclpeocoaxialfibersenhancesmscproliferationandfibrogenesis AT mingdongdong dualdeliveryoffgf2ctgffromsilkfibroinplclpeocoaxialfibersenhancesmscproliferationandfibrogenesis AT flemmingbesenbacher dualdeliveryoffgf2ctgffromsilkfibroinplclpeocoaxialfibersenhancesmscproliferationandfibrogenesis AT menglinchen dualdeliveryoffgf2ctgffromsilkfibroinplclpeocoaxialfibersenhancesmscproliferationandfibrogenesis
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Dual-delivery of FGF-2/CTGF from Silk Fibroin/PLCL-PEO Coaxial Fibers Enhances MSC Proliferation and Fibrogenesis

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