GPI-80 Augments NF-κB Activation in Tumor Cells

Recent studies have discovered a relationship between glycosylphosphatidylinositol (GPI)-anchored protein 80 (GPI-80)/VNN2 (80 kDa GPI-anchored protein) and malignant tumors. GPI-80 is known to regulate neutrophil adhesion; however, the action of GPI-80 on tumors is still obscure. In this study, alt...

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Autores principales:	Yuji Takeda, Yuta Kurota, Tomoyuki Kato, Hiromi Ito, Akemi Araki, Hidetoshi Nara, Shinichi Saitoh, Nobuyuki Tanaka, Norihiko Tsuchiya, Hironobu Asao
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	IL-1β NF-κB oxidative stress pantetheinase prostate cancer cells Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/a5810e6cad8b464db0454c85916ff062
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spelling	oai:doaj.org-article:a5810e6cad8b464db0454c85916ff0622021-11-11T17:25:45ZGPI-80 Augments NF-κB Activation in Tumor Cells10.3390/ijms2221120271422-00671661-6596https://doaj.org/article/a5810e6cad8b464db0454c85916ff0622021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/12027https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Recent studies have discovered a relationship between glycosylphosphatidylinositol (GPI)-anchored protein 80 (GPI-80)/VNN2 (80 kDa GPI-anchored protein) and malignant tumors. GPI-80 is known to regulate neutrophil adhesion; however, the action of GPI-80 on tumors is still obscure. In this study, although the expression of GPI-80 mRNA was detectable in several tumor cell lines, the levels of GPI-80 protein were significantly lower than that in neutrophils. To clarify the function of GPI-80 in tumor cells, GPI-80-expressing cells and GPI-80/VNN2 gene-deleted cells were established using PC3 prostate cancer cells. In GPI-80-expressing cells, GPI-80 was mainly detected in vesicles. Furthermore, soluble GPI-80 in the conditioned medium was associated with the exosome marker CD63 and was also detected in the plasma obtained from prostate cancer patients. Unexpectedly, cell adhesion and migration of GPI-80-expressing PC3 cells were not modulated by anti-GPI-80 antibody treatment. However, similar to the GPI-80 family molecule, VNN1, the pantetheinase activity and oxidative state were augmented in GPI-80-expressing cells. GPI-80-expressing cells facilitated non-adhesive proliferation, slow cell proliferation, NF-κB activation and IL-1β production. These phenomena are known to be induced by physiological elevation of the oxidative state. Thus, these observations indicated that GPI-80 affects various tumor responses related to oxidation.Yuji TakedaYuta KurotaTomoyuki KatoHiromi ItoAkemi ArakiHidetoshi NaraShinichi SaitohNobuyuki TanakaNorihiko TsuchiyaHironobu AsaoMDPI AGarticleIL-1βNF-κBoxidative stresspantetheinaseprostate cancer cellsBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12027, p 12027 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	IL-1β NF-κB oxidative stress pantetheinase prostate cancer cells Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	IL-1β NF-κB oxidative stress pantetheinase prostate cancer cells Biology (General) QH301-705.5 Chemistry QD1-999 Yuji Takeda Yuta Kurota Tomoyuki Kato Hiromi Ito Akemi Araki Hidetoshi Nara Shinichi Saitoh Nobuyuki Tanaka Norihiko Tsuchiya Hironobu Asao GPI-80 Augments NF-κB Activation in Tumor Cells
description	Recent studies have discovered a relationship between glycosylphosphatidylinositol (GPI)-anchored protein 80 (GPI-80)/VNN2 (80 kDa GPI-anchored protein) and malignant tumors. GPI-80 is known to regulate neutrophil adhesion; however, the action of GPI-80 on tumors is still obscure. In this study, although the expression of GPI-80 mRNA was detectable in several tumor cell lines, the levels of GPI-80 protein were significantly lower than that in neutrophils. To clarify the function of GPI-80 in tumor cells, GPI-80-expressing cells and GPI-80/VNN2 gene-deleted cells were established using PC3 prostate cancer cells. In GPI-80-expressing cells, GPI-80 was mainly detected in vesicles. Furthermore, soluble GPI-80 in the conditioned medium was associated with the exosome marker CD63 and was also detected in the plasma obtained from prostate cancer patients. Unexpectedly, cell adhesion and migration of GPI-80-expressing PC3 cells were not modulated by anti-GPI-80 antibody treatment. However, similar to the GPI-80 family molecule, VNN1, the pantetheinase activity and oxidative state were augmented in GPI-80-expressing cells. GPI-80-expressing cells facilitated non-adhesive proliferation, slow cell proliferation, NF-κB activation and IL-1β production. These phenomena are known to be induced by physiological elevation of the oxidative state. Thus, these observations indicated that GPI-80 affects various tumor responses related to oxidation.
format	article
author	Yuji Takeda Yuta Kurota Tomoyuki Kato Hiromi Ito Akemi Araki Hidetoshi Nara Shinichi Saitoh Nobuyuki Tanaka Norihiko Tsuchiya Hironobu Asao
author_facet	Yuji Takeda Yuta Kurota Tomoyuki Kato Hiromi Ito Akemi Araki Hidetoshi Nara Shinichi Saitoh Nobuyuki Tanaka Norihiko Tsuchiya Hironobu Asao
author_sort	Yuji Takeda
title	GPI-80 Augments NF-κB Activation in Tumor Cells
title_short	GPI-80 Augments NF-κB Activation in Tumor Cells
title_full	GPI-80 Augments NF-κB Activation in Tumor Cells
title_fullStr	GPI-80 Augments NF-κB Activation in Tumor Cells
title_full_unstemmed	GPI-80 Augments NF-κB Activation in Tumor Cells
title_sort	gpi-80 augments nf-κb activation in tumor cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a5810e6cad8b464db0454c85916ff062
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GPI-80 Augments NF-κB Activation in Tumor Cells

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