Microbial communities in polychlorinated biphenyl (PCB)-contaminated wastewater lagoon sediments: PCB congener, quantitative PCR, and 16S rRNA gene amplicon sequencing datasets

The potential for aerobic and anaerobic microbial natural attenuation of PCBs in freshwater sediments is described by PCB congener, quantitative PCR, and 16S rRNA gene amplicon sequencing datasets generated, in duplicate, from 27 sediment samples collected from a PCB-contaminated freshwater lagoon (...

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Autores principales:	Timothy E. Mattes, Jessica M. Ewald, Yi Liang, Andres Martinez, Andrew M. Awad, Keri C. Hornbuckle, Jerald L. Schnoor
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Polychlorinated biphenyls Wastewater lagoon sediments Reductive dehalogenation Dehalococcoides 16S rRNA gene sequencing Quantitative PCR Computer applications to medicine. Medical informatics R858-859.7 Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/a583ef64edfe4677b93a5a69e050d1f0
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spelling	oai:doaj.org-article:a583ef64edfe4677b93a5a69e050d1f02021-11-12T04:37:51ZMicrobial communities in polychlorinated biphenyl (PCB)-contaminated wastewater lagoon sediments: PCB congener, quantitative PCR, and 16S rRNA gene amplicon sequencing datasets2352-340910.1016/j.dib.2021.107546https://doaj.org/article/a583ef64edfe4677b93a5a69e050d1f02021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2352340921008222https://doaj.org/toc/2352-3409The potential for aerobic and anaerobic microbial natural attenuation of PCBs in freshwater sediments is described by PCB congener, quantitative PCR, and 16S rRNA gene amplicon sequencing datasets generated, in duplicate, from 27 sediment samples collected from a PCB-contaminated freshwater lagoon (54 samples total). Sediment samples were subjected to a hexane PCB extraction protocol and the concentrations of 209 PCB congeners were determined in hexane extracts by gas chromatography with a tandem mass spectrometry detection. DNA was extracted from sediments sediment samples and used for qPCR and 16S rRNA amplicon sequencing. The abundance of 16S rRNA genes (i.e., Dehalococcoides and putative dechlorinating Chloroflexi) and functional genes (i.e., reductive dehalogenase (rdhA) and biphenyl dioxygenase (bphA)) associated with aerobic and anaerobic PCB biodegradation, along with the total 16S rRNA genes abundance, was determined by SYBR green qPCR. The microbial community composition and structure in all sediment samples was obtained by 16S rRNA gene amplicon sequencing. Primers targeting the 16S rRNA gene V4 region were used to produce 16S rRNA gene amplicons that were sequencing with the high-throughput Illumina MiSeq platform and sequencing chemistry. The 16S rRNA gene sequencing dataset along with PCB congener and qPCR datasets included as metadata, could be reused in meta-analyses that aim to determine microbial community interactions in contaminated environments, and uncover relationships between microbial community structure and environmental variable (e.g., PCB congener concentrations).Timothy E. MattesJessica M. EwaldYi LiangAndres MartinezAndrew M. AwadKeri C. HornbuckleJerald L. SchnoorElsevierarticlePolychlorinated biphenylsWastewater lagoon sedimentsReductive dehalogenationDehalococcoides16S rRNA gene sequencingQuantitative PCRComputer applications to medicine. Medical informaticsR858-859.7Science (General)Q1-390ENData in Brief, Vol 39, Iss , Pp 107546- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Polychlorinated biphenyls Wastewater lagoon sediments Reductive dehalogenation Dehalococcoides 16S rRNA gene sequencing Quantitative PCR Computer applications to medicine. Medical informatics R858-859.7 Science (General) Q1-390
spellingShingle	Polychlorinated biphenyls Wastewater lagoon sediments Reductive dehalogenation Dehalococcoides 16S rRNA gene sequencing Quantitative PCR Computer applications to medicine. Medical informatics R858-859.7 Science (General) Q1-390 Timothy E. Mattes Jessica M. Ewald Yi Liang Andres Martinez Andrew M. Awad Keri C. Hornbuckle Jerald L. Schnoor Microbial communities in polychlorinated biphenyl (PCB)-contaminated wastewater lagoon sediments: PCB congener, quantitative PCR, and 16S rRNA gene amplicon sequencing datasets
description	The potential for aerobic and anaerobic microbial natural attenuation of PCBs in freshwater sediments is described by PCB congener, quantitative PCR, and 16S rRNA gene amplicon sequencing datasets generated, in duplicate, from 27 sediment samples collected from a PCB-contaminated freshwater lagoon (54 samples total). Sediment samples were subjected to a hexane PCB extraction protocol and the concentrations of 209 PCB congeners were determined in hexane extracts by gas chromatography with a tandem mass spectrometry detection. DNA was extracted from sediments sediment samples and used for qPCR and 16S rRNA amplicon sequencing. The abundance of 16S rRNA genes (i.e., Dehalococcoides and putative dechlorinating Chloroflexi) and functional genes (i.e., reductive dehalogenase (rdhA) and biphenyl dioxygenase (bphA)) associated with aerobic and anaerobic PCB biodegradation, along with the total 16S rRNA genes abundance, was determined by SYBR green qPCR. The microbial community composition and structure in all sediment samples was obtained by 16S rRNA gene amplicon sequencing. Primers targeting the 16S rRNA gene V4 region were used to produce 16S rRNA gene amplicons that were sequencing with the high-throughput Illumina MiSeq platform and sequencing chemistry. The 16S rRNA gene sequencing dataset along with PCB congener and qPCR datasets included as metadata, could be reused in meta-analyses that aim to determine microbial community interactions in contaminated environments, and uncover relationships between microbial community structure and environmental variable (e.g., PCB congener concentrations).
format	article
author	Timothy E. Mattes Jessica M. Ewald Yi Liang Andres Martinez Andrew M. Awad Keri C. Hornbuckle Jerald L. Schnoor
author_facet	Timothy E. Mattes Jessica M. Ewald Yi Liang Andres Martinez Andrew M. Awad Keri C. Hornbuckle Jerald L. Schnoor
author_sort	Timothy E. Mattes
title	Microbial communities in polychlorinated biphenyl (PCB)-contaminated wastewater lagoon sediments: PCB congener, quantitative PCR, and 16S rRNA gene amplicon sequencing datasets
title_short	Microbial communities in polychlorinated biphenyl (PCB)-contaminated wastewater lagoon sediments: PCB congener, quantitative PCR, and 16S rRNA gene amplicon sequencing datasets
title_full	Microbial communities in polychlorinated biphenyl (PCB)-contaminated wastewater lagoon sediments: PCB congener, quantitative PCR, and 16S rRNA gene amplicon sequencing datasets
title_fullStr	Microbial communities in polychlorinated biphenyl (PCB)-contaminated wastewater lagoon sediments: PCB congener, quantitative PCR, and 16S rRNA gene amplicon sequencing datasets
title_full_unstemmed	Microbial communities in polychlorinated biphenyl (PCB)-contaminated wastewater lagoon sediments: PCB congener, quantitative PCR, and 16S rRNA gene amplicon sequencing datasets
title_sort	microbial communities in polychlorinated biphenyl (pcb)-contaminated wastewater lagoon sediments: pcb congener, quantitative pcr, and 16s rrna gene amplicon sequencing datasets
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/a583ef64edfe4677b93a5a69e050d1f0
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Microbial communities in polychlorinated biphenyl (PCB)-contaminated wastewater lagoon sediments: PCB congener, quantitative PCR, and 16S rRNA gene amplicon sequencing datasets

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