Natural variations in the biofilm-associated protein BslA from the genus Bacillus

Abstract BslA is a protein secreted by Bacillus subtilis which forms a hydrophobic film that coats the biofilm surface and renders it water-repellent. We have characterised three orthologues of BslA from Bacillus amyloliquefaciens, Bacillus licheniformis and Bacillus pumilus as well as a paralogue f...

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Autores principales:	Ryan J. Morris, Marieke Schor, Rachel M. C. Gillespie, Ana Sofia Ferreira, Lucia Baldauf, Chris Earl, Adam Ostrowski, Laura Hobley, Keith M. Bromley, Tetyana Sukhodub, Sofia Arnaouteli, Nicola R. Stanley-Wall, Cait E. MacPhee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a586664c221344d0a32082a119a55168
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spelling	oai:doaj.org-article:a586664c221344d0a32082a119a551682021-12-02T11:53:04ZNatural variations in the biofilm-associated protein BslA from the genus Bacillus10.1038/s41598-017-06786-92045-2322https://doaj.org/article/a586664c221344d0a32082a119a551682017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06786-9https://doaj.org/toc/2045-2322Abstract BslA is a protein secreted by Bacillus subtilis which forms a hydrophobic film that coats the biofilm surface and renders it water-repellent. We have characterised three orthologues of BslA from Bacillus amyloliquefaciens, Bacillus licheniformis and Bacillus pumilus as well as a paralogue from B. subtilis called YweA. We find that the three orthologous proteins can substitute for BslA in B. subtilis and confer a degree of protection, whereas YweA cannot. The degree to which the proteins functionally substitute for native BslA correlates with their in vitro biophysical properties. Our results demonstrate the use of naturally-evolved variants to provide a framework for teasing out the molecular basis of interfacial self-assembly.Ryan J. MorrisMarieke SchorRachel M. C. GillespieAna Sofia FerreiraLucia BaldaufChris EarlAdam OstrowskiLaura HobleyKeith M. BromleyTetyana SukhodubSofia ArnaouteliNicola R. Stanley-WallCait E. MacPheeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ryan J. Morris Marieke Schor Rachel M. C. Gillespie Ana Sofia Ferreira Lucia Baldauf Chris Earl Adam Ostrowski Laura Hobley Keith M. Bromley Tetyana Sukhodub Sofia Arnaouteli Nicola R. Stanley-Wall Cait E. MacPhee Natural variations in the biofilm-associated protein BslA from the genus Bacillus
description	Abstract BslA is a protein secreted by Bacillus subtilis which forms a hydrophobic film that coats the biofilm surface and renders it water-repellent. We have characterised three orthologues of BslA from Bacillus amyloliquefaciens, Bacillus licheniformis and Bacillus pumilus as well as a paralogue from B. subtilis called YweA. We find that the three orthologous proteins can substitute for BslA in B. subtilis and confer a degree of protection, whereas YweA cannot. The degree to which the proteins functionally substitute for native BslA correlates with their in vitro biophysical properties. Our results demonstrate the use of naturally-evolved variants to provide a framework for teasing out the molecular basis of interfacial self-assembly.
format	article
author	Ryan J. Morris Marieke Schor Rachel M. C. Gillespie Ana Sofia Ferreira Lucia Baldauf Chris Earl Adam Ostrowski Laura Hobley Keith M. Bromley Tetyana Sukhodub Sofia Arnaouteli Nicola R. Stanley-Wall Cait E. MacPhee
author_facet	Ryan J. Morris Marieke Schor Rachel M. C. Gillespie Ana Sofia Ferreira Lucia Baldauf Chris Earl Adam Ostrowski Laura Hobley Keith M. Bromley Tetyana Sukhodub Sofia Arnaouteli Nicola R. Stanley-Wall Cait E. MacPhee
author_sort	Ryan J. Morris
title	Natural variations in the biofilm-associated protein BslA from the genus Bacillus
title_short	Natural variations in the biofilm-associated protein BslA from the genus Bacillus
title_full	Natural variations in the biofilm-associated protein BslA from the genus Bacillus
title_fullStr	Natural variations in the biofilm-associated protein BslA from the genus Bacillus
title_full_unstemmed	Natural variations in the biofilm-associated protein BslA from the genus Bacillus
title_sort	natural variations in the biofilm-associated protein bsla from the genus bacillus
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a586664c221344d0a32082a119a55168
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Natural variations in the biofilm-associated protein BslA from the genus Bacillus

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