Selective Inhibition of IL-6 Trans-Signaling Has No Beneficial Effect on the Posttraumatic Cytokine Release after Multiple Trauma in Mice

While improvements in pre-hospital and in-hospital care allow more multiple trauma patients to advance to intensive care, the incidence of posttraumatic multiple organ dysfunction syndrome (MODS) is on the rise. Herein, the influence of a selective IL-6 trans-signaling inhibition on posttraumatic cy...

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Autores principales:	Jil-Madeline Homeier, Katrin Bundkirchen, Marcel Winkelmann, Tilman Graulich, Borna Relja, Claudia Neunaber, Christian Macke
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	multiple trauma MODS sgp130Fc trans-signaling inflammatory cytokines Science Q
Acceso en línea:	https://doaj.org/article/a58d6cc9e9ee402693e996a4d191012b
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spelling	oai:doaj.org-article:a58d6cc9e9ee402693e996a4d191012b2021-11-25T18:11:35ZSelective Inhibition of IL-6 Trans-Signaling Has No Beneficial Effect on the Posttraumatic Cytokine Release after Multiple Trauma in Mice10.3390/life111112522075-1729https://doaj.org/article/a58d6cc9e9ee402693e996a4d191012b2021-11-01T00:00:00Zhttps://www.mdpi.com/2075-1729/11/11/1252https://doaj.org/toc/2075-1729While improvements in pre-hospital and in-hospital care allow more multiple trauma patients to advance to intensive care, the incidence of posttraumatic multiple organ dysfunction syndrome (MODS) is on the rise. Herein, the influence of a selective IL-6 trans-signaling inhibition on posttraumatic cytokine levels was investigated as an approach to prevent MODS caused by a dysbalanced posttraumatic immune reaction. Therefore, the artificial IL-6 trans-signaling inhibitor sgp130Fc was deployed in a murine multiple trauma model (femoral fracture plus bilateral chest trauma). The traumatized mice were treated with sgp130Fc (FP) and compared to untreated mice (WT) and IL-6 receptor knockout mice (RKO), which received the same traumas. The overall trauma mortality was 4.4%. Microscopic pulmonary changes were apparent after multiple trauma and after isolated bilateral chest trauma. Elevated IL-6, MCP-3 and RANTES plasma levels were measured after trauma, indicating a successful induction of a systemic inflammatory reaction. Significantly reduced IL-6 and RANTES plasma levels were visible in RKO compared to WT. Only a little effect was visible in FP compared to WT. Comparable cytokine levels in WT and FP indicate neither a protective nor an adverse effect of sgp130Fc on the cytokine release after femoral fracture and bilateral chest trauma.Jil-Madeline HomeierKatrin BundkirchenMarcel WinkelmannTilman GraulichBorna ReljaClaudia NeunaberChristian MackeMDPI AGarticlemultiple traumaMODSsgp130Fctrans-signalinginflammatory cytokinesScienceQENLife, Vol 11, Iss 1252, p 1252 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	multiple trauma MODS sgp130Fc trans-signaling inflammatory cytokines Science Q
spellingShingle	multiple trauma MODS sgp130Fc trans-signaling inflammatory cytokines Science Q Jil-Madeline Homeier Katrin Bundkirchen Marcel Winkelmann Tilman Graulich Borna Relja Claudia Neunaber Christian Macke Selective Inhibition of IL-6 Trans-Signaling Has No Beneficial Effect on the Posttraumatic Cytokine Release after Multiple Trauma in Mice
description	While improvements in pre-hospital and in-hospital care allow more multiple trauma patients to advance to intensive care, the incidence of posttraumatic multiple organ dysfunction syndrome (MODS) is on the rise. Herein, the influence of a selective IL-6 trans-signaling inhibition on posttraumatic cytokine levels was investigated as an approach to prevent MODS caused by a dysbalanced posttraumatic immune reaction. Therefore, the artificial IL-6 trans-signaling inhibitor sgp130Fc was deployed in a murine multiple trauma model (femoral fracture plus bilateral chest trauma). The traumatized mice were treated with sgp130Fc (FP) and compared to untreated mice (WT) and IL-6 receptor knockout mice (RKO), which received the same traumas. The overall trauma mortality was 4.4%. Microscopic pulmonary changes were apparent after multiple trauma and after isolated bilateral chest trauma. Elevated IL-6, MCP-3 and RANTES plasma levels were measured after trauma, indicating a successful induction of a systemic inflammatory reaction. Significantly reduced IL-6 and RANTES plasma levels were visible in RKO compared to WT. Only a little effect was visible in FP compared to WT. Comparable cytokine levels in WT and FP indicate neither a protective nor an adverse effect of sgp130Fc on the cytokine release after femoral fracture and bilateral chest trauma.
format	article
author	Jil-Madeline Homeier Katrin Bundkirchen Marcel Winkelmann Tilman Graulich Borna Relja Claudia Neunaber Christian Macke
author_facet	Jil-Madeline Homeier Katrin Bundkirchen Marcel Winkelmann Tilman Graulich Borna Relja Claudia Neunaber Christian Macke
author_sort	Jil-Madeline Homeier
title	Selective Inhibition of IL-6 Trans-Signaling Has No Beneficial Effect on the Posttraumatic Cytokine Release after Multiple Trauma in Mice
title_short	Selective Inhibition of IL-6 Trans-Signaling Has No Beneficial Effect on the Posttraumatic Cytokine Release after Multiple Trauma in Mice
title_full	Selective Inhibition of IL-6 Trans-Signaling Has No Beneficial Effect on the Posttraumatic Cytokine Release after Multiple Trauma in Mice
title_fullStr	Selective Inhibition of IL-6 Trans-Signaling Has No Beneficial Effect on the Posttraumatic Cytokine Release after Multiple Trauma in Mice
title_full_unstemmed	Selective Inhibition of IL-6 Trans-Signaling Has No Beneficial Effect on the Posttraumatic Cytokine Release after Multiple Trauma in Mice
title_sort	selective inhibition of il-6 trans-signaling has no beneficial effect on the posttraumatic cytokine release after multiple trauma in mice
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a58d6cc9e9ee402693e996a4d191012b
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Selective Inhibition of IL-6 Trans-Signaling Has No Beneficial Effect on the Posttraumatic Cytokine Release after Multiple Trauma in Mice

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