Therapeutic Effect of Stem Cells on Male Infertility in a Rat Model: Histological, Molecular, Biochemical, and Functional Study

Methotrexate (MTX) is a folic acid antagonist, widely used as a chemotherapeutic and immunosuppressive drug, but it is toxic to reproductive systems. In recent years, the era of stem cell applications becomes a promising point as a possible therapeutic agent in male infertility. This study is aimed...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sally S. Mohammed, Mona F. Mansour, Noha A. Salem
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Internal medicine RC31-1245
Acceso en línea:	https://doaj.org/article/a59ac5f32332438e839385618e370d9e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a59ac5f32332438e839385618e370d9e
record_format	dspace
spelling	oai:doaj.org-article:a59ac5f32332438e839385618e370d9e2021-11-08T02:35:31ZTherapeutic Effect of Stem Cells on Male Infertility in a Rat Model: Histological, Molecular, Biochemical, and Functional Study1687-967810.1155/2021/8450721https://doaj.org/article/a59ac5f32332438e839385618e370d9e2021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/8450721https://doaj.org/toc/1687-9678Methotrexate (MTX) is a folic acid antagonist, widely used as a chemotherapeutic and immunosuppressive drug, but it is toxic to reproductive systems. In recent years, the era of stem cell applications becomes a promising point as a possible therapeutic agent in male infertility. This study is aimed at evaluating the therapeutic effects of stem cells at histological, molecular, biochemical, and functional levels in a methotrexate-induced testicular damage model. Material and Methods. Thirty rats were divided randomly into three groups (ten rats each): group 1 (control): animals received an intraperitoneal injection of 2 ml phosphate-buffered saline per week for 4 weeks, group 2 (MTX-treated group): animals were intraperitoneally injected with methotrexate (8 mg/kg) once weekly for 4 weeks, and group 3 (ADMSC-treated group): methotrexate-treated animals received a single dose of 1×106 stem cells/rat at the 5th week. At the 8th week, blood samples were collected for hormonal analysis; then, animals were sacrificed. The testes were dissected; the right testis was stained with hematoxylin and eosin. Random sections were taken from group 3 and examined with a fluorescent microscope. The left testis was divided into two specimens: the first was used for an electron microscope and the second was homogenized for molecular and biochemical assessments. Results. Group 2 showed significant histological changes, decreased free testosterone level, decrease in stem cell factor expression, and dysfunction of the oxidation state. The results revealed significant improvement of these parameters. Conclusion. Transplantation of adipose tissue-derived stem cells (ADMSCs) can improve the testicular damage histologically and functionally in a rat model.Sally S. MohammedMona F. MansourNoha A. SalemHindawi LimitedarticleInternal medicineRC31-1245ENStem Cells International, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Internal medicine RC31-1245
spellingShingle	Internal medicine RC31-1245 Sally S. Mohammed Mona F. Mansour Noha A. Salem Therapeutic Effect of Stem Cells on Male Infertility in a Rat Model: Histological, Molecular, Biochemical, and Functional Study
description	Methotrexate (MTX) is a folic acid antagonist, widely used as a chemotherapeutic and immunosuppressive drug, but it is toxic to reproductive systems. In recent years, the era of stem cell applications becomes a promising point as a possible therapeutic agent in male infertility. This study is aimed at evaluating the therapeutic effects of stem cells at histological, molecular, biochemical, and functional levels in a methotrexate-induced testicular damage model. Material and Methods. Thirty rats were divided randomly into three groups (ten rats each): group 1 (control): animals received an intraperitoneal injection of 2 ml phosphate-buffered saline per week for 4 weeks, group 2 (MTX-treated group): animals were intraperitoneally injected with methotrexate (8 mg/kg) once weekly for 4 weeks, and group 3 (ADMSC-treated group): methotrexate-treated animals received a single dose of 1×106 stem cells/rat at the 5th week. At the 8th week, blood samples were collected for hormonal analysis; then, animals were sacrificed. The testes were dissected; the right testis was stained with hematoxylin and eosin. Random sections were taken from group 3 and examined with a fluorescent microscope. The left testis was divided into two specimens: the first was used for an electron microscope and the second was homogenized for molecular and biochemical assessments. Results. Group 2 showed significant histological changes, decreased free testosterone level, decrease in stem cell factor expression, and dysfunction of the oxidation state. The results revealed significant improvement of these parameters. Conclusion. Transplantation of adipose tissue-derived stem cells (ADMSCs) can improve the testicular damage histologically and functionally in a rat model.
format	article
author	Sally S. Mohammed Mona F. Mansour Noha A. Salem
author_facet	Sally S. Mohammed Mona F. Mansour Noha A. Salem
author_sort	Sally S. Mohammed
title	Therapeutic Effect of Stem Cells on Male Infertility in a Rat Model: Histological, Molecular, Biochemical, and Functional Study
title_short	Therapeutic Effect of Stem Cells on Male Infertility in a Rat Model: Histological, Molecular, Biochemical, and Functional Study
title_full	Therapeutic Effect of Stem Cells on Male Infertility in a Rat Model: Histological, Molecular, Biochemical, and Functional Study
title_fullStr	Therapeutic Effect of Stem Cells on Male Infertility in a Rat Model: Histological, Molecular, Biochemical, and Functional Study
title_full_unstemmed	Therapeutic Effect of Stem Cells on Male Infertility in a Rat Model: Histological, Molecular, Biochemical, and Functional Study
title_sort	therapeutic effect of stem cells on male infertility in a rat model: histological, molecular, biochemical, and functional study
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/a59ac5f32332438e839385618e370d9e
work_keys_str_mv	AT sallysmohammed therapeuticeffectofstemcellsonmaleinfertilityinaratmodelhistologicalmolecularbiochemicalandfunctionalstudy AT monafmansour therapeuticeffectofstemcellsonmaleinfertilityinaratmodelhistologicalmolecularbiochemicalandfunctionalstudy AT nohaasalem therapeuticeffectofstemcellsonmaleinfertilityinaratmodelhistologicalmolecularbiochemicalandfunctionalstudy
_version_	1718443216032759808

Therapeutic Effect of Stem Cells on Male Infertility in a Rat Model: Histological, Molecular, Biochemical, and Functional Study

Ejemplares similares