Synthesis-dependent strand annealing in meiosis.

Recent studies led to the proposal that meiotic gene conversion can result after transient engagement of the donor chromatid and subsequent DNA synthesis-dependent strand annealing (SDSA). Double Holliday junction (dHJ) intermediates were previously proposed to form both reciprocal crossover recombi...

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Autores principales:	Melissa S McMahill, Caroline W Sham, Douglas K Bishop
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2007
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a59d11ce63364b18b66b02a5159f637f
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id	oai:doaj.org-article:a59d11ce63364b18b66b02a5159f637f
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spelling	oai:doaj.org-article:a59d11ce63364b18b66b02a5159f637f2021-11-25T05:33:05ZSynthesis-dependent strand annealing in meiosis.1544-91731545-788510.1371/journal.pbio.0050299https://doaj.org/article/a59d11ce63364b18b66b02a5159f637f2007-11-01T00:00:00Zhttps://doi.org/10.1371/journal.pbio.0050299https://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Recent studies led to the proposal that meiotic gene conversion can result after transient engagement of the donor chromatid and subsequent DNA synthesis-dependent strand annealing (SDSA). Double Holliday junction (dHJ) intermediates were previously proposed to form both reciprocal crossover recombinants (COs) and noncrossover recombinants (NCOs); however, dHJs are now thought to give rise mainly to COs, with SDSA forming most or all NCOs. To test this model in Saccharomyces cerevisiae, we constructed a random spore system in which it is possible to identify a subset of NCO recombinants that can readily be accounted for by SDSA, but not by dHJ-mediated recombination. The diagnostic class of recombinants is one in which two markers on opposite sides of a double-strand break site are converted, without conversion of an intervening heterologous insertion located on the donor chromatid. This diagnostic class represents 26% of selected NCO recombinants. Tetrad analysis using the same markers provided additional evidence that SDSA is a major pathway for NCO gene conversion in meiosis.Melissa S McMahillCaroline W ShamDouglas K BishopPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 5, Iss 11, p e299 (2007)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Melissa S McMahill Caroline W Sham Douglas K Bishop Synthesis-dependent strand annealing in meiosis.
description	Recent studies led to the proposal that meiotic gene conversion can result after transient engagement of the donor chromatid and subsequent DNA synthesis-dependent strand annealing (SDSA). Double Holliday junction (dHJ) intermediates were previously proposed to form both reciprocal crossover recombinants (COs) and noncrossover recombinants (NCOs); however, dHJs are now thought to give rise mainly to COs, with SDSA forming most or all NCOs. To test this model in Saccharomyces cerevisiae, we constructed a random spore system in which it is possible to identify a subset of NCO recombinants that can readily be accounted for by SDSA, but not by dHJ-mediated recombination. The diagnostic class of recombinants is one in which two markers on opposite sides of a double-strand break site are converted, without conversion of an intervening heterologous insertion located on the donor chromatid. This diagnostic class represents 26% of selected NCO recombinants. Tetrad analysis using the same markers provided additional evidence that SDSA is a major pathway for NCO gene conversion in meiosis.
format	article
author	Melissa S McMahill Caroline W Sham Douglas K Bishop
author_facet	Melissa S McMahill Caroline W Sham Douglas K Bishop
author_sort	Melissa S McMahill
title	Synthesis-dependent strand annealing in meiosis.
title_short	Synthesis-dependent strand annealing in meiosis.
title_full	Synthesis-dependent strand annealing in meiosis.
title_fullStr	Synthesis-dependent strand annealing in meiosis.
title_full_unstemmed	Synthesis-dependent strand annealing in meiosis.
title_sort	synthesis-dependent strand annealing in meiosis.
publisher	Public Library of Science (PLoS)
publishDate	2007
url	https://doaj.org/article/a59d11ce63364b18b66b02a5159f637f
work_keys_str_mv	AT melissasmcmahill synthesisdependentstrandannealinginmeiosis AT carolinewsham synthesisdependentstrandannealinginmeiosis AT douglaskbishop synthesisdependentstrandannealinginmeiosis
_version_	1718414657459322880

Synthesis-dependent strand annealing in meiosis.

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