Transcriptome profiling of bovine milk oligosaccharide metabolism genes using RNA-sequencing.

This study examines the genes coding for enzymes involved in bovine milk oligosaccharide metabolism by comparing the oligosaccharide profiles with the expressions of glycosylation-related genes. Fresh milk samples (n = 32) were collected from four Holstein and Jersey cows at days 1, 15, 90 and 250 o...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Saumya Wickramasinghe, Serenus Hua, Gonzalo Rincon, Alma Islas-Trejo, J Bruce German, Carlito B Lebrilla, Juan F Medrano
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a5a2a0e9482447f9a6b2ce99427bcd65
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a5a2a0e9482447f9a6b2ce99427bcd65
record_format	dspace
spelling	oai:doaj.org-article:a5a2a0e9482447f9a6b2ce99427bcd652021-11-18T06:55:16ZTranscriptome profiling of bovine milk oligosaccharide metabolism genes using RNA-sequencing.1932-620310.1371/journal.pone.0018895https://doaj.org/article/a5a2a0e9482447f9a6b2ce99427bcd652011-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21541029/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203This study examines the genes coding for enzymes involved in bovine milk oligosaccharide metabolism by comparing the oligosaccharide profiles with the expressions of glycosylation-related genes. Fresh milk samples (n = 32) were collected from four Holstein and Jersey cows at days 1, 15, 90 and 250 of lactation and free milk oligosaccharide profiles were analyzed. RNA was extracted from milk somatic cells at days 15 and 250 of lactation (n = 12) and gene expression analysis was conducted by RNA-Sequencing. A list was created of 121 glycosylation-related genes involved in oligosaccharide metabolism pathways in bovine by analyzing the oligosaccharide profiles and performing an extensive literature search. No significant differences were observed in either oligosaccharide profiles or expressions of glycosylation-related genes between Holstein and Jersey cows. The highest concentrations of free oligosaccharides were observed in the colostrum samples and a sharp decrease was observed in the concentration of free oligosaccharides on day 15, followed by progressive decrease on days 90 and 250. Ninety-two glycosylation-related genes were expressed in milk somatic cells. Most of these genes exhibited higher expression in day 250 samples indicating increases in net glycosylation-related metabolism in spite of decreases in free milk oligosaccharides in late lactation milk. Even though fucosylated free oligosaccharides were not identified, gene expression indicated the likely presence of fucosylated oligosaccharides in bovine milk. Fucosidase genes were expressed in milk and a possible explanation for not detecting fucosylated free oligosaccharides is the degradation of large fucosylated free oligosaccharides by the fucosidases. Detailed characterization of enzymes encoded by the 92 glycosylation-related genes identified in this study will provide the basic knowledge for metabolic network analysis of oligosaccharides in mammalian milk. These candidate genes will guide the design of a targeted breeding strategy to optimize the content of beneficial oligosaccharides in bovine milk.Saumya WickramasingheSerenus HuaGonzalo RinconAlma Islas-TrejoJ Bruce GermanCarlito B LebrillaJuan F MedranoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 4, p e18895 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Saumya Wickramasinghe Serenus Hua Gonzalo Rincon Alma Islas-Trejo J Bruce German Carlito B Lebrilla Juan F Medrano Transcriptome profiling of bovine milk oligosaccharide metabolism genes using RNA-sequencing.
description	This study examines the genes coding for enzymes involved in bovine milk oligosaccharide metabolism by comparing the oligosaccharide profiles with the expressions of glycosylation-related genes. Fresh milk samples (n = 32) were collected from four Holstein and Jersey cows at days 1, 15, 90 and 250 of lactation and free milk oligosaccharide profiles were analyzed. RNA was extracted from milk somatic cells at days 15 and 250 of lactation (n = 12) and gene expression analysis was conducted by RNA-Sequencing. A list was created of 121 glycosylation-related genes involved in oligosaccharide metabolism pathways in bovine by analyzing the oligosaccharide profiles and performing an extensive literature search. No significant differences were observed in either oligosaccharide profiles or expressions of glycosylation-related genes between Holstein and Jersey cows. The highest concentrations of free oligosaccharides were observed in the colostrum samples and a sharp decrease was observed in the concentration of free oligosaccharides on day 15, followed by progressive decrease on days 90 and 250. Ninety-two glycosylation-related genes were expressed in milk somatic cells. Most of these genes exhibited higher expression in day 250 samples indicating increases in net glycosylation-related metabolism in spite of decreases in free milk oligosaccharides in late lactation milk. Even though fucosylated free oligosaccharides were not identified, gene expression indicated the likely presence of fucosylated oligosaccharides in bovine milk. Fucosidase genes were expressed in milk and a possible explanation for not detecting fucosylated free oligosaccharides is the degradation of large fucosylated free oligosaccharides by the fucosidases. Detailed characterization of enzymes encoded by the 92 glycosylation-related genes identified in this study will provide the basic knowledge for metabolic network analysis of oligosaccharides in mammalian milk. These candidate genes will guide the design of a targeted breeding strategy to optimize the content of beneficial oligosaccharides in bovine milk.
format	article
author	Saumya Wickramasinghe Serenus Hua Gonzalo Rincon Alma Islas-Trejo J Bruce German Carlito B Lebrilla Juan F Medrano
author_facet	Saumya Wickramasinghe Serenus Hua Gonzalo Rincon Alma Islas-Trejo J Bruce German Carlito B Lebrilla Juan F Medrano
author_sort	Saumya Wickramasinghe
title	Transcriptome profiling of bovine milk oligosaccharide metabolism genes using RNA-sequencing.
title_short	Transcriptome profiling of bovine milk oligosaccharide metabolism genes using RNA-sequencing.
title_full	Transcriptome profiling of bovine milk oligosaccharide metabolism genes using RNA-sequencing.
title_fullStr	Transcriptome profiling of bovine milk oligosaccharide metabolism genes using RNA-sequencing.
title_full_unstemmed	Transcriptome profiling of bovine milk oligosaccharide metabolism genes using RNA-sequencing.
title_sort	transcriptome profiling of bovine milk oligosaccharide metabolism genes using rna-sequencing.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/a5a2a0e9482447f9a6b2ce99427bcd65
work_keys_str_mv	AT saumyawickramasinghe transcriptomeprofilingofbovinemilkoligosaccharidemetabolismgenesusingrnasequencing AT serenushua transcriptomeprofilingofbovinemilkoligosaccharidemetabolismgenesusingrnasequencing AT gonzalorincon transcriptomeprofilingofbovinemilkoligosaccharidemetabolismgenesusingrnasequencing AT almaislastrejo transcriptomeprofilingofbovinemilkoligosaccharidemetabolismgenesusingrnasequencing AT jbrucegerman transcriptomeprofilingofbovinemilkoligosaccharidemetabolismgenesusingrnasequencing AT carlitoblebrilla transcriptomeprofilingofbovinemilkoligosaccharidemetabolismgenesusingrnasequencing AT juanfmedrano transcriptomeprofilingofbovinemilkoligosaccharidemetabolismgenesusingrnasequencing
_version_	1718424184800935936

Transcriptome profiling of bovine milk oligosaccharide metabolism genes using RNA-sequencing.

Ejemplares similares