Nitrite-producing oral microbiome in adults and children

Abstract Recently, it was suggested that the nitrite (NO2 −) produced from NO3 − by oral bacteria might contribute to oral and general health. Therefore, we aimed to clarify the detailed information about the bacterial NO2-production in the oral biofilm. Dental plaque and tongue-coating samples were...

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Autores principales:	Yuria Sato-Suzuki, Jumpei Washio, Dimas Prasetianto Wicaksono, Takuichi Sato, Satoshi Fukumoto, Nobuhiro Takahashi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a5aa5e47e9794da4bda5cd88ba354ed4
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spelling	oai:doaj.org-article:a5aa5e47e9794da4bda5cd88ba354ed42021-12-02T18:37:07ZNitrite-producing oral microbiome in adults and children10.1038/s41598-020-73479-12045-2322https://doaj.org/article/a5aa5e47e9794da4bda5cd88ba354ed42020-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-73479-1https://doaj.org/toc/2045-2322Abstract Recently, it was suggested that the nitrite (NO2 −) produced from NO3 − by oral bacteria might contribute to oral and general health. Therefore, we aimed to clarify the detailed information about the bacterial NO2-production in the oral biofilm. Dental plaque and tongue-coating samples were collected, then the NO2-producing activity was measured. Furthermore, the composition of the NO2 −-producing bacterial population were identified using the Griess reagent-containing agar overlay method and molecular biological method. NO2 −-producing activity per mg wet weight varied among individuals but was higher in dental plaque. Additionally, anaerobic bacteria exhibited higher numbers of NO2 −-producing bacteria, except in the adults’ dental plaque. The proportion of NO2 −-producing bacteria also varied among individuals, but a positive correlation was found between NO2 −-producing activity and the number of NO2 −-producing bacteria, especially in dental plaque. Overall, the major NO2 −-producing bacteria were identified as Actinomyces, Schaalia, Veillonella and Neisseria. Furthermore, Rothia was specifically detected in the tongue coatings of children. These results suggest that dental plaque has higher NO2 −-producing activity and that this activity depends not on the presence of specific bacteria or the bacterial compositions, but on the number of NO2 −-producing bacteria, although interindividual differences were detected.Yuria Sato-SuzukiJumpei WashioDimas Prasetianto WicaksonoTakuichi SatoSatoshi FukumotoNobuhiro TakahashiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuria Sato-Suzuki Jumpei Washio Dimas Prasetianto Wicaksono Takuichi Sato Satoshi Fukumoto Nobuhiro Takahashi Nitrite-producing oral microbiome in adults and children
description	Abstract Recently, it was suggested that the nitrite (NO2 −) produced from NO3 − by oral bacteria might contribute to oral and general health. Therefore, we aimed to clarify the detailed information about the bacterial NO2-production in the oral biofilm. Dental plaque and tongue-coating samples were collected, then the NO2-producing activity was measured. Furthermore, the composition of the NO2 −-producing bacterial population were identified using the Griess reagent-containing agar overlay method and molecular biological method. NO2 −-producing activity per mg wet weight varied among individuals but was higher in dental plaque. Additionally, anaerobic bacteria exhibited higher numbers of NO2 −-producing bacteria, except in the adults’ dental plaque. The proportion of NO2 −-producing bacteria also varied among individuals, but a positive correlation was found between NO2 −-producing activity and the number of NO2 −-producing bacteria, especially in dental plaque. Overall, the major NO2 −-producing bacteria were identified as Actinomyces, Schaalia, Veillonella and Neisseria. Furthermore, Rothia was specifically detected in the tongue coatings of children. These results suggest that dental plaque has higher NO2 −-producing activity and that this activity depends not on the presence of specific bacteria or the bacterial compositions, but on the number of NO2 −-producing bacteria, although interindividual differences were detected.
format	article
author	Yuria Sato-Suzuki Jumpei Washio Dimas Prasetianto Wicaksono Takuichi Sato Satoshi Fukumoto Nobuhiro Takahashi
author_facet	Yuria Sato-Suzuki Jumpei Washio Dimas Prasetianto Wicaksono Takuichi Sato Satoshi Fukumoto Nobuhiro Takahashi
author_sort	Yuria Sato-Suzuki
title	Nitrite-producing oral microbiome in adults and children
title_short	Nitrite-producing oral microbiome in adults and children
title_full	Nitrite-producing oral microbiome in adults and children
title_fullStr	Nitrite-producing oral microbiome in adults and children
title_full_unstemmed	Nitrite-producing oral microbiome in adults and children
title_sort	nitrite-producing oral microbiome in adults and children
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/a5aa5e47e9794da4bda5cd88ba354ed4
work_keys_str_mv	AT yuriasatosuzuki nitriteproducingoralmicrobiomeinadultsandchildren AT jumpeiwashio nitriteproducingoralmicrobiomeinadultsandchildren AT dimasprasetiantowicaksono nitriteproducingoralmicrobiomeinadultsandchildren AT takuichisato nitriteproducingoralmicrobiomeinadultsandchildren AT satoshifukumoto nitriteproducingoralmicrobiomeinadultsandchildren AT nobuhirotakahashi nitriteproducingoralmicrobiomeinadultsandchildren
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Nitrite-producing oral microbiome in adults and children

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