A novel adenylate isopentenyltransferase 5 regulates shoot branching via the ATTTA motif in Camellia sinensis

Abstract Background Shoot branching is one of the important agronomic traits affecting yields and quality of tea plant (Camellia sinensis). Cytokinins (CTKs) play critical roles in regulating shoot branching. However, whether and how differently alternative splicing (AS) variant of CTKs-related gene...

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Autores principales:	Liping Zhang, Menghan Li, Peng Yan, Jianyu Fu, Lan Zhang, Xin Li, Wenyan Han
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Adenylate isopentenyltransferase ATTTA motif Alternative splicing (AS) variant Axillary bud Cytokinins (CTKs) Tea plant Botany QK1-989
Acceso en línea:	https://doaj.org/article/a5ad9863634149caab2a3fb46a580fc3
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spelling	oai:doaj.org-article:a5ad9863634149caab2a3fb46a580fc32021-11-14T12:09:25ZA novel adenylate isopentenyltransferase 5 regulates shoot branching via the ATTTA motif in Camellia sinensis10.1186/s12870-021-03254-51471-2229https://doaj.org/article/a5ad9863634149caab2a3fb46a580fc32021-11-01T00:00:00Zhttps://doi.org/10.1186/s12870-021-03254-5https://doaj.org/toc/1471-2229Abstract Background Shoot branching is one of the important agronomic traits affecting yields and quality of tea plant (Camellia sinensis). Cytokinins (CTKs) play critical roles in regulating shoot branching. However, whether and how differently alternative splicing (AS) variant of CTKs-related genes can influence shoot branching of tea plant is still not fully elucidated. Results In this study, five AS variants of CTK biosynthetic gene adenylate isopentenyltransferase (CsA-IPT5) with different 3′ untranslated region (3ˊ UTR) and 5ˊ UTR from tea plant were cloned and investigated for their regulatory effects. Transient expression assays showed that there were significant negative correlations between CsA-IPT5 protein expression, mRNA expression of CsA-IPT5 AS variants and the number of ATTTA motifs, respectively. Shoot branching processes induced by exogenous 6-BA or pruning were studied, where CsA-IPT5 was demonstrated to regulate protein synthesis of CsA-IPT5, as well as the biosynthesis of trans-zeatin (tZ)- and isopentenyladenine (iP)-CTKs, through transcriptionally changing ratios of its five AS variants in these processes. Furthermore, the 3′ UTR AS variant 2 (3AS2) might act as the predominant AS transcript. Conclusions Together, our results indicate that 3AS2 of the CsA-IPT5 gene is potential in regulating shoot branching of tea plant and provides a gene resource for improving the plant-type of woody plants.Liping ZhangMenghan LiPeng YanJianyu FuLan ZhangXin LiWenyan HanBMCarticleAdenylate isopentenyltransferaseATTTA motifAlternative splicing (AS) variantAxillary budCytokinins (CTKs)Tea plantBotanyQK1-989ENBMC Plant Biology, Vol 21, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Adenylate isopentenyltransferase ATTTA motif Alternative splicing (AS) variant Axillary bud Cytokinins (CTKs) Tea plant Botany QK1-989
spellingShingle	Adenylate isopentenyltransferase ATTTA motif Alternative splicing (AS) variant Axillary bud Cytokinins (CTKs) Tea plant Botany QK1-989 Liping Zhang Menghan Li Peng Yan Jianyu Fu Lan Zhang Xin Li Wenyan Han A novel adenylate isopentenyltransferase 5 regulates shoot branching via the ATTTA motif in Camellia sinensis
description	Abstract Background Shoot branching is one of the important agronomic traits affecting yields and quality of tea plant (Camellia sinensis). Cytokinins (CTKs) play critical roles in regulating shoot branching. However, whether and how differently alternative splicing (AS) variant of CTKs-related genes can influence shoot branching of tea plant is still not fully elucidated. Results In this study, five AS variants of CTK biosynthetic gene adenylate isopentenyltransferase (CsA-IPT5) with different 3′ untranslated region (3ˊ UTR) and 5ˊ UTR from tea plant were cloned and investigated for their regulatory effects. Transient expression assays showed that there were significant negative correlations between CsA-IPT5 protein expression, mRNA expression of CsA-IPT5 AS variants and the number of ATTTA motifs, respectively. Shoot branching processes induced by exogenous 6-BA or pruning were studied, where CsA-IPT5 was demonstrated to regulate protein synthesis of CsA-IPT5, as well as the biosynthesis of trans-zeatin (tZ)- and isopentenyladenine (iP)-CTKs, through transcriptionally changing ratios of its five AS variants in these processes. Furthermore, the 3′ UTR AS variant 2 (3AS2) might act as the predominant AS transcript. Conclusions Together, our results indicate that 3AS2 of the CsA-IPT5 gene is potential in regulating shoot branching of tea plant and provides a gene resource for improving the plant-type of woody plants.
format	article
author	Liping Zhang Menghan Li Peng Yan Jianyu Fu Lan Zhang Xin Li Wenyan Han
author_facet	Liping Zhang Menghan Li Peng Yan Jianyu Fu Lan Zhang Xin Li Wenyan Han
author_sort	Liping Zhang
title	A novel adenylate isopentenyltransferase 5 regulates shoot branching via the ATTTA motif in Camellia sinensis
title_short	A novel adenylate isopentenyltransferase 5 regulates shoot branching via the ATTTA motif in Camellia sinensis
title_full	A novel adenylate isopentenyltransferase 5 regulates shoot branching via the ATTTA motif in Camellia sinensis
title_fullStr	A novel adenylate isopentenyltransferase 5 regulates shoot branching via the ATTTA motif in Camellia sinensis
title_full_unstemmed	A novel adenylate isopentenyltransferase 5 regulates shoot branching via the ATTTA motif in Camellia sinensis
title_sort	novel adenylate isopentenyltransferase 5 regulates shoot branching via the attta motif in camellia sinensis
publisher	BMC
publishDate	2021
url	https://doaj.org/article/a5ad9863634149caab2a3fb46a580fc3
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A novel adenylate isopentenyltransferase 5 regulates shoot branching via the ATTTA motif in Camellia sinensis

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