Prospective genotyping of Mycobacterium tuberculosis from fresh clinical samples.

Shorter time-to-result is key for improving molecular-guided epidemiological investigation of tuberculosis (TB) cases. We performed a prospective study to evaluate the use of standardized MIRU-VNTR (mycobacterial interspersed repetitive-unit-variable-number tandem-repeat) typing of Mycobacterium tub...

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Autores principales:	Urška Bidovec-Stojkovič, Katja Seme, Manca Žolnir-Dovč, Philip Supply
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a5aff1cffd1b46d182dd1e8c1f004550
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spelling	oai:doaj.org-article:a5aff1cffd1b46d182dd1e8c1f0045502021-11-25T05:56:42ZProspective genotyping of Mycobacterium tuberculosis from fresh clinical samples.1932-620310.1371/journal.pone.0109547https://doaj.org/article/a5aff1cffd1b46d182dd1e8c1f0045502014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0109547https://doaj.org/toc/1932-6203Shorter time-to-result is key for improving molecular-guided epidemiological investigation of tuberculosis (TB) cases. We performed a prospective study to evaluate the use of standardized MIRU-VNTR (mycobacterial interspersed repetitive-unit-variable-number tandem-repeat) typing of Mycobacterium tuberculosis directly on 79 fresh clinical samples from 26 TB patients consecutively enrolled over a 17-month period. Overall, complete 24-locus types were obtained for 18 out of the 26 (69.2%) patients and 14 of the 16 grade 3+ and grade 2+ samples (87.5%). The degree of completion of the genotypes obtained significantly correlated with smear microscopy grade both for 26 first samples (p = 0.0003) and for 53 follow-up samples (p = 0.002). For 20 of the 26 patients for whom complete or even incomplete M. tuberculosis isolate genotypes were obtained, typing applied to the clinical samples allowed the same unambiguous conclusions regarding case clustering or uniqueness as those that could have been drawn based on the corresponding cultured isolates. Standard 24 locus MIRU-VNTR typing of M. tuberculosis can be applied directly to fresh clinical samples, with typeability depending on the bacterial load in the sample.Urška Bidovec-StojkovičKatja SemeManca Žolnir-DovčPhilip SupplyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 10, p e109547 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Urška Bidovec-Stojkovič Katja Seme Manca Žolnir-Dovč Philip Supply Prospective genotyping of Mycobacterium tuberculosis from fresh clinical samples.
description	Shorter time-to-result is key for improving molecular-guided epidemiological investigation of tuberculosis (TB) cases. We performed a prospective study to evaluate the use of standardized MIRU-VNTR (mycobacterial interspersed repetitive-unit-variable-number tandem-repeat) typing of Mycobacterium tuberculosis directly on 79 fresh clinical samples from 26 TB patients consecutively enrolled over a 17-month period. Overall, complete 24-locus types were obtained for 18 out of the 26 (69.2%) patients and 14 of the 16 grade 3+ and grade 2+ samples (87.5%). The degree of completion of the genotypes obtained significantly correlated with smear microscopy grade both for 26 first samples (p = 0.0003) and for 53 follow-up samples (p = 0.002). For 20 of the 26 patients for whom complete or even incomplete M. tuberculosis isolate genotypes were obtained, typing applied to the clinical samples allowed the same unambiguous conclusions regarding case clustering or uniqueness as those that could have been drawn based on the corresponding cultured isolates. Standard 24 locus MIRU-VNTR typing of M. tuberculosis can be applied directly to fresh clinical samples, with typeability depending on the bacterial load in the sample.
format	article
author	Urška Bidovec-Stojkovič Katja Seme Manca Žolnir-Dovč Philip Supply
author_facet	Urška Bidovec-Stojkovič Katja Seme Manca Žolnir-Dovč Philip Supply
author_sort	Urška Bidovec-Stojkovič
title	Prospective genotyping of Mycobacterium tuberculosis from fresh clinical samples.
title_short	Prospective genotyping of Mycobacterium tuberculosis from fresh clinical samples.
title_full	Prospective genotyping of Mycobacterium tuberculosis from fresh clinical samples.
title_fullStr	Prospective genotyping of Mycobacterium tuberculosis from fresh clinical samples.
title_full_unstemmed	Prospective genotyping of Mycobacterium tuberculosis from fresh clinical samples.
title_sort	prospective genotyping of mycobacterium tuberculosis from fresh clinical samples.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/a5aff1cffd1b46d182dd1e8c1f004550
work_keys_str_mv	AT urskabidovecstojkovic prospectivegenotypingofmycobacteriumtuberculosisfromfreshclinicalsamples AT katjaseme prospectivegenotypingofmycobacteriumtuberculosisfromfreshclinicalsamples AT mancazolnirdovc prospectivegenotypingofmycobacteriumtuberculosisfromfreshclinicalsamples AT philipsupply prospectivegenotypingofmycobacteriumtuberculosisfromfreshclinicalsamples
_version_	1718414358129672192

Prospective genotyping of Mycobacterium tuberculosis from fresh clinical samples.

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