Erythropoietin ameliorates diabetes-associated cognitive dysfunction in vitro and in vivo

Abstract Several studies indicate that erythropoietin (EPO) has remarkable neuroprotective effects in various central nervous system disorders, while little is known about the effects of EPO in diabetes-associated cognitive dysfunction. Therefore, the present study aimed to investigate whether EPO a...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Meng Wang, Wenhui Yan, Yuan Liu, Hao Hu, Qiang Sun, Xinlin Chen, Weijin Zang, Lina Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a5b10f1360684aad8b1da859e2ea4d52
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a5b10f1360684aad8b1da859e2ea4d52
record_format	dspace
spelling	oai:doaj.org-article:a5b10f1360684aad8b1da859e2ea4d522021-12-02T15:06:17ZErythropoietin ameliorates diabetes-associated cognitive dysfunction in vitro and in vivo10.1038/s41598-017-03137-62045-2322https://doaj.org/article/a5b10f1360684aad8b1da859e2ea4d522017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03137-6https://doaj.org/toc/2045-2322Abstract Several studies indicate that erythropoietin (EPO) has remarkable neuroprotective effects in various central nervous system disorders, while little is known about the effects of EPO in diabetes-associated cognitive dysfunction. Therefore, the present study aimed to investigate whether EPO ameliorates diabetes-associated cognitive dysfunction in vivo and in vitro. We investigated the protective effects of EPO on high-glucose (HG)-induced PC12 cell death and oxidative stress. The effects of EPO (300 U/kg administered three times a week for 4 weeks) on diabetes-associated cognitive decline were investigated in diabetic rats. EPO significantly increased cell viability, increased the activity of superoxide dismutase, decreased the production of malondialdehyde and reactive oxygen species, and decreased the apoptosis rate. Additionally, LY294002, a phosphatidylinositol 3-kinase (PI3K) inhibitor, abolished the protective effects of EPO in HG-treated PC12 cells. In diabetic rats, EPO prevented deficits in spatial learning and memory in the Morris water maze test. The results of real-time PCR and Western blotting showed that EPO upregulated EPO receptor, PI3K, and phosphorylated Akt2 relative to unphosphorylated Akt2 (p-Akt2/Akt2) and downregulated glycogen synthase kinase-3β (GSK-3β). These studies demonstrate that EPO is an effective neuroprotective agent in the context of diabetes-associated cognitive dysfunction and show that this effect involves the PI3K/Akt/GSK-3β pathway.Meng WangWenhui YanYuan LiuHao HuQiang SunXinlin ChenWeijin ZangLina ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Meng Wang Wenhui Yan Yuan Liu Hao Hu Qiang Sun Xinlin Chen Weijin Zang Lina Chen Erythropoietin ameliorates diabetes-associated cognitive dysfunction in vitro and in vivo
description	Abstract Several studies indicate that erythropoietin (EPO) has remarkable neuroprotective effects in various central nervous system disorders, while little is known about the effects of EPO in diabetes-associated cognitive dysfunction. Therefore, the present study aimed to investigate whether EPO ameliorates diabetes-associated cognitive dysfunction in vivo and in vitro. We investigated the protective effects of EPO on high-glucose (HG)-induced PC12 cell death and oxidative stress. The effects of EPO (300 U/kg administered three times a week for 4 weeks) on diabetes-associated cognitive decline were investigated in diabetic rats. EPO significantly increased cell viability, increased the activity of superoxide dismutase, decreased the production of malondialdehyde and reactive oxygen species, and decreased the apoptosis rate. Additionally, LY294002, a phosphatidylinositol 3-kinase (PI3K) inhibitor, abolished the protective effects of EPO in HG-treated PC12 cells. In diabetic rats, EPO prevented deficits in spatial learning and memory in the Morris water maze test. The results of real-time PCR and Western blotting showed that EPO upregulated EPO receptor, PI3K, and phosphorylated Akt2 relative to unphosphorylated Akt2 (p-Akt2/Akt2) and downregulated glycogen synthase kinase-3β (GSK-3β). These studies demonstrate that EPO is an effective neuroprotective agent in the context of diabetes-associated cognitive dysfunction and show that this effect involves the PI3K/Akt/GSK-3β pathway.
format	article
author	Meng Wang Wenhui Yan Yuan Liu Hao Hu Qiang Sun Xinlin Chen Weijin Zang Lina Chen
author_facet	Meng Wang Wenhui Yan Yuan Liu Hao Hu Qiang Sun Xinlin Chen Weijin Zang Lina Chen
author_sort	Meng Wang
title	Erythropoietin ameliorates diabetes-associated cognitive dysfunction in vitro and in vivo
title_short	Erythropoietin ameliorates diabetes-associated cognitive dysfunction in vitro and in vivo
title_full	Erythropoietin ameliorates diabetes-associated cognitive dysfunction in vitro and in vivo
title_fullStr	Erythropoietin ameliorates diabetes-associated cognitive dysfunction in vitro and in vivo
title_full_unstemmed	Erythropoietin ameliorates diabetes-associated cognitive dysfunction in vitro and in vivo
title_sort	erythropoietin ameliorates diabetes-associated cognitive dysfunction in vitro and in vivo
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a5b10f1360684aad8b1da859e2ea4d52
work_keys_str_mv	AT mengwang erythropoietinamelioratesdiabetesassociatedcognitivedysfunctioninvitroandinvivo AT wenhuiyan erythropoietinamelioratesdiabetesassociatedcognitivedysfunctioninvitroandinvivo AT yuanliu erythropoietinamelioratesdiabetesassociatedcognitivedysfunctioninvitroandinvivo AT haohu erythropoietinamelioratesdiabetesassociatedcognitivedysfunctioninvitroandinvivo AT qiangsun erythropoietinamelioratesdiabetesassociatedcognitivedysfunctioninvitroandinvivo AT xinlinchen erythropoietinamelioratesdiabetesassociatedcognitivedysfunctioninvitroandinvivo AT weijinzang erythropoietinamelioratesdiabetesassociatedcognitivedysfunctioninvitroandinvivo AT linachen erythropoietinamelioratesdiabetesassociatedcognitivedysfunctioninvitroandinvivo
_version_	1718388534846423040

Erythropoietin ameliorates diabetes-associated cognitive dysfunction in vitro and in vivo

Ejemplares similares