Genome-Wide Analysis of Alternative Splicing and Non-Coding RNAs Reveal Complicated Transcriptional Regulation in <i>Cannabis sativa</i> L.

It is of significance to mine the structural genes related to the biosynthetic pathway of fatty acid (FA) and cellulose as well as explore the regulatory mechanism of alternative splicing (AS), microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) in the biosynthesis of cannabinoids, FA and cellulos...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Bin Wu, Yanni Li, Jishuang Li, Zhenzhen Xie, Mingbao Luan, Chunsheng Gao, Yuhua Shi, Shilin Chen
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Cannabis sativa</i> L. alternative splicing non-coding RNAs gene expression and regulation Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/a5b8b493c5ee485590f2279a9e12f698
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a5b8b493c5ee485590f2279a9e12f698
record_format	dspace
spelling	oai:doaj.org-article:a5b8b493c5ee485590f2279a9e12f6982021-11-11T17:25:21ZGenome-Wide Analysis of Alternative Splicing and Non-Coding RNAs Reveal Complicated Transcriptional Regulation in <i>Cannabis sativa</i> L.10.3390/ijms2221119891422-00671661-6596https://doaj.org/article/a5b8b493c5ee485590f2279a9e12f6982021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11989https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067It is of significance to mine the structural genes related to the biosynthetic pathway of fatty acid (FA) and cellulose as well as explore the regulatory mechanism of alternative splicing (AS), microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) in the biosynthesis of cannabinoids, FA and cellulose, which would enhance the knowledge of gene expression and regulation at post-transcriptional level in <i>Cannabis sativa</i> L. In this study, transcriptome, small RNA and degradome libraries of hemp ‘Yunma No.1’ were established, and comprehensive analysis was performed. As a result, a total of 154, 32 and 331 transcripts encoding key enzymes involved in the biosynthesis of cannabinoids, FA and cellulose were predicted, respectively, among which AS occurred in 368 transcripts. Moreover, 183 conserved miRNAs, 380 <i>C. sativa</i>-specific miRNAs and 7783 lncRNAs were predicted. Among them, 70 miRNAs and 17 lncRNAs potentially targeted 13 and 17 transcripts, respectively, encoding key enzymes or transporters involved in the biosynthesis of cannabinoids, cellulose or FA. Finally, the crosstalk between AS and miRNAs or lncRNAs involved in cannabinoids and cellulose was also predicted. In summary, all these results provided insights into the complicated network of gene expression and regulation in <i>C. sativa</i>.Bin WuYanni LiJishuang LiZhenzhen XieMingbao LuanChunsheng GaoYuhua ShiShilin ChenMDPI AGarticle<i>Cannabis sativa</i> L.alternative splicingnon-coding RNAsgene expression and regulationBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11989, p 11989 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Cannabis sativa</i> L. alternative splicing non-coding RNAs gene expression and regulation Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	<i>Cannabis sativa</i> L. alternative splicing non-coding RNAs gene expression and regulation Biology (General) QH301-705.5 Chemistry QD1-999 Bin Wu Yanni Li Jishuang Li Zhenzhen Xie Mingbao Luan Chunsheng Gao Yuhua Shi Shilin Chen Genome-Wide Analysis of Alternative Splicing and Non-Coding RNAs Reveal Complicated Transcriptional Regulation in <i>Cannabis sativa</i> L.
description	It is of significance to mine the structural genes related to the biosynthetic pathway of fatty acid (FA) and cellulose as well as explore the regulatory mechanism of alternative splicing (AS), microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) in the biosynthesis of cannabinoids, FA and cellulose, which would enhance the knowledge of gene expression and regulation at post-transcriptional level in <i>Cannabis sativa</i> L. In this study, transcriptome, small RNA and degradome libraries of hemp ‘Yunma No.1’ were established, and comprehensive analysis was performed. As a result, a total of 154, 32 and 331 transcripts encoding key enzymes involved in the biosynthesis of cannabinoids, FA and cellulose were predicted, respectively, among which AS occurred in 368 transcripts. Moreover, 183 conserved miRNAs, 380 <i>C. sativa</i>-specific miRNAs and 7783 lncRNAs were predicted. Among them, 70 miRNAs and 17 lncRNAs potentially targeted 13 and 17 transcripts, respectively, encoding key enzymes or transporters involved in the biosynthesis of cannabinoids, cellulose or FA. Finally, the crosstalk between AS and miRNAs or lncRNAs involved in cannabinoids and cellulose was also predicted. In summary, all these results provided insights into the complicated network of gene expression and regulation in <i>C. sativa</i>.
format	article
author	Bin Wu Yanni Li Jishuang Li Zhenzhen Xie Mingbao Luan Chunsheng Gao Yuhua Shi Shilin Chen
author_facet	Bin Wu Yanni Li Jishuang Li Zhenzhen Xie Mingbao Luan Chunsheng Gao Yuhua Shi Shilin Chen
author_sort	Bin Wu
title	Genome-Wide Analysis of Alternative Splicing and Non-Coding RNAs Reveal Complicated Transcriptional Regulation in <i>Cannabis sativa</i> L.
title_short	Genome-Wide Analysis of Alternative Splicing and Non-Coding RNAs Reveal Complicated Transcriptional Regulation in <i>Cannabis sativa</i> L.
title_full	Genome-Wide Analysis of Alternative Splicing and Non-Coding RNAs Reveal Complicated Transcriptional Regulation in <i>Cannabis sativa</i> L.
title_fullStr	Genome-Wide Analysis of Alternative Splicing and Non-Coding RNAs Reveal Complicated Transcriptional Regulation in <i>Cannabis sativa</i> L.
title_full_unstemmed	Genome-Wide Analysis of Alternative Splicing and Non-Coding RNAs Reveal Complicated Transcriptional Regulation in <i>Cannabis sativa</i> L.
title_sort	genome-wide analysis of alternative splicing and non-coding rnas reveal complicated transcriptional regulation in <i>cannabis sativa</i> l.
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a5b8b493c5ee485590f2279a9e12f698
work_keys_str_mv	AT binwu genomewideanalysisofalternativesplicingandnoncodingrnasrevealcomplicatedtranscriptionalregulationinicannabissativail AT yannili genomewideanalysisofalternativesplicingandnoncodingrnasrevealcomplicatedtranscriptionalregulationinicannabissativail AT jishuangli genomewideanalysisofalternativesplicingandnoncodingrnasrevealcomplicatedtranscriptionalregulationinicannabissativail AT zhenzhenxie genomewideanalysisofalternativesplicingandnoncodingrnasrevealcomplicatedtranscriptionalregulationinicannabissativail AT mingbaoluan genomewideanalysisofalternativesplicingandnoncodingrnasrevealcomplicatedtranscriptionalregulationinicannabissativail AT chunshenggao genomewideanalysisofalternativesplicingandnoncodingrnasrevealcomplicatedtranscriptionalregulationinicannabissativail AT yuhuashi genomewideanalysisofalternativesplicingandnoncodingrnasrevealcomplicatedtranscriptionalregulationinicannabissativail AT shilinchen genomewideanalysisofalternativesplicingandnoncodingrnasrevealcomplicatedtranscriptionalregulationinicannabissativail
_version_	1718432136808103936

Genome-Wide Analysis of Alternative Splicing and Non-Coding RNAs Reveal Complicated Transcriptional Regulation in <i>Cannabis sativa</i> L.

Ejemplares similares