Age-Related Variation of Bacterial and Fungal Communities in Different Body Habitats across the Young, Elderly, and Centenarians in Sardinia

ABSTRACT Human body microbes interact with the host, forming microbial communities that are in continual flux during the aging process. Previous studies have mostly focused on surveying a single body habitat to determine the age-related variation in the bacterial and fungal communities. A more compr...

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Autores principales:	Lu Wu, Tiansheng Zeng, Massimo Deligios, Luciano Milanesi, Morgan G. I. Langille, Angelo Zinellu, Salvatore Rubino, Ciriaco Carru, David J. Kelvin
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	16S rRNA sequencing ITS1 sequencing Sardinian aging gut microbiota human microbiome Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/a5b9ffc85a324834bc4fff8bd64bcdba
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spelling	oai:doaj.org-article:a5b9ffc85a324834bc4fff8bd64bcdba2021-11-15T15:27:53ZAge-Related Variation of Bacterial and Fungal Communities in Different Body Habitats across the Young, Elderly, and Centenarians in Sardinia10.1128/mSphere.00558-192379-5042https://doaj.org/article/a5b9ffc85a324834bc4fff8bd64bcdba2020-02-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00558-19https://doaj.org/toc/2379-5042ABSTRACT Human body microbes interact with the host, forming microbial communities that are in continual flux during the aging process. Previous studies have mostly focused on surveying a single body habitat to determine the age-related variation in the bacterial and fungal communities. A more comprehensive understanding of the variation in the human microbiota and mycobiota across multiple body habitats related to aging is still unclear. To obtain an integrated view of the spatial distribution of microbes in a specific Mediterranean population across a wide age range, we surveyed the bacterial and fungal communities in the skin, oral cavity, and gut in the young, elderly, and centenarians in Sardinia using 16S rRNA gene and internal transcribed spacer 1 (ITS1) sequencing. We found that the distribution and correlation of bacterial and fungal communities in Sardinians were largely determined by body site. In each age group, the bacterial and fungal communities found in the skin were significantly different in structure. In the oral cavity, age had a marginal impact on the structures of the bacterial and fungal communities. Furthermore, the gut bacterial communities in centenarians clustered separately from those of the young and elderly, while the fungal communities in the gut habitat could not be separated by host age. IMPORTANCE Site-specific microbial communities are recognized as important factors in host health and disease. To better understand how the human microbiota potentially affects and is affected by its host during the aging process, the fundamental issue to address is the distribution of microbiota related to age. Here, we show an integrated view of the spatial distribution of microbes in a specific Mediterranean population (Sardinians) across a wide age range. Our study indicates that age plays a critical role in shaping the human microbiota in a habitat-dependent manner. The dynamic age-related microbiota changes we observed across multiple body sites may provide possibilities for modulating microbe communities to maintain or improve health during aging.Lu WuTiansheng ZengMassimo DeligiosLuciano MilanesiMorgan G. I. LangilleAngelo ZinelluSalvatore RubinoCiriaco CarruDavid J. KelvinAmerican Society for Microbiologyarticle16S rRNA sequencingITS1 sequencingSardinianaginggut microbiotahuman microbiomeMicrobiologyQR1-502ENmSphere, Vol 5, Iss 1 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	16S rRNA sequencing ITS1 sequencing Sardinian aging gut microbiota human microbiome Microbiology QR1-502
spellingShingle	16S rRNA sequencing ITS1 sequencing Sardinian aging gut microbiota human microbiome Microbiology QR1-502 Lu Wu Tiansheng Zeng Massimo Deligios Luciano Milanesi Morgan G. I. Langille Angelo Zinellu Salvatore Rubino Ciriaco Carru David J. Kelvin Age-Related Variation of Bacterial and Fungal Communities in Different Body Habitats across the Young, Elderly, and Centenarians in Sardinia
description	ABSTRACT Human body microbes interact with the host, forming microbial communities that are in continual flux during the aging process. Previous studies have mostly focused on surveying a single body habitat to determine the age-related variation in the bacterial and fungal communities. A more comprehensive understanding of the variation in the human microbiota and mycobiota across multiple body habitats related to aging is still unclear. To obtain an integrated view of the spatial distribution of microbes in a specific Mediterranean population across a wide age range, we surveyed the bacterial and fungal communities in the skin, oral cavity, and gut in the young, elderly, and centenarians in Sardinia using 16S rRNA gene and internal transcribed spacer 1 (ITS1) sequencing. We found that the distribution and correlation of bacterial and fungal communities in Sardinians were largely determined by body site. In each age group, the bacterial and fungal communities found in the skin were significantly different in structure. In the oral cavity, age had a marginal impact on the structures of the bacterial and fungal communities. Furthermore, the gut bacterial communities in centenarians clustered separately from those of the young and elderly, while the fungal communities in the gut habitat could not be separated by host age. IMPORTANCE Site-specific microbial communities are recognized as important factors in host health and disease. To better understand how the human microbiota potentially affects and is affected by its host during the aging process, the fundamental issue to address is the distribution of microbiota related to age. Here, we show an integrated view of the spatial distribution of microbes in a specific Mediterranean population (Sardinians) across a wide age range. Our study indicates that age plays a critical role in shaping the human microbiota in a habitat-dependent manner. The dynamic age-related microbiota changes we observed across multiple body sites may provide possibilities for modulating microbe communities to maintain or improve health during aging.
format	article
author	Lu Wu Tiansheng Zeng Massimo Deligios Luciano Milanesi Morgan G. I. Langille Angelo Zinellu Salvatore Rubino Ciriaco Carru David J. Kelvin
author_facet	Lu Wu Tiansheng Zeng Massimo Deligios Luciano Milanesi Morgan G. I. Langille Angelo Zinellu Salvatore Rubino Ciriaco Carru David J. Kelvin
author_sort	Lu Wu
title	Age-Related Variation of Bacterial and Fungal Communities in Different Body Habitats across the Young, Elderly, and Centenarians in Sardinia
title_short	Age-Related Variation of Bacterial and Fungal Communities in Different Body Habitats across the Young, Elderly, and Centenarians in Sardinia
title_full	Age-Related Variation of Bacterial and Fungal Communities in Different Body Habitats across the Young, Elderly, and Centenarians in Sardinia
title_fullStr	Age-Related Variation of Bacterial and Fungal Communities in Different Body Habitats across the Young, Elderly, and Centenarians in Sardinia
title_full_unstemmed	Age-Related Variation of Bacterial and Fungal Communities in Different Body Habitats across the Young, Elderly, and Centenarians in Sardinia
title_sort	age-related variation of bacterial and fungal communities in different body habitats across the young, elderly, and centenarians in sardinia
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/a5b9ffc85a324834bc4fff8bd64bcdba
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Age-Related Variation of Bacterial and Fungal Communities in Different Body Habitats across the Young, Elderly, and Centenarians in Sardinia

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