Early evaluation of corneal collagen crosslinking in ex-vivo human corneas using two-photon imaging

Abstract The clinical outcome of corneal collagen crosslinking (CXL) is typically evaluated several weeks after treatment. An earlier assessment of its outcome could lead to an optimization of the treatment, including an immediate re-intervention in case of failure, thereby, avoiding additional disc...

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Autores principales:	Ana Batista, Hans Georg Breunig, Tobias Hager, Berthold Seitz, Karsten König
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a5c6042916684329aae24e8bedde2402
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spelling	oai:doaj.org-article:a5c6042916684329aae24e8bedde24022021-12-02T15:09:46ZEarly evaluation of corneal collagen crosslinking in ex-vivo human corneas using two-photon imaging10.1038/s41598-019-46572-32045-2322https://doaj.org/article/a5c6042916684329aae24e8bedde24022019-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-46572-3https://doaj.org/toc/2045-2322Abstract The clinical outcome of corneal collagen crosslinking (CXL) is typically evaluated several weeks after treatment. An earlier assessment of its outcome could lead to an optimization of the treatment, including an immediate re-intervention in case of failure, thereby, avoiding additional discomfort and pain to the patient. In this study, we propose two-photon imaging (TPI) as an earlier evaluation method. CXL was performed in human corneas by application of riboflavin followed by UVA irradiation. Autofluorescence (AF) intensity and lifetime images were acquired using a commercial clinically certified multiphoton tomograph prior to CXL and after 2h, 24h, 72h, and 144h storage in culture medium. The first monitoring point was determined as the minimum time required for riboflavin clearance from the cornea. As control, untreated samples and samples treated only with riboflavin (without UVA irradiation) were monitored at the same time points. Significant increases in the stroma AF intensity and lifetime were observed as soon as 2h after treatment. A depth-dependent TPI analysis showed higher AF lifetimes anteriorly corresponding to areas were CXL was most effective. No alterations were observed in the control groups. Using TPI, the outcome of CXL can be assessed non-invasively and label-free much sooner than with conventional clinical devices.Ana BatistaHans Georg BreunigTobias HagerBerthold SeitzKarsten KönigNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-9 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ana Batista Hans Georg Breunig Tobias Hager Berthold Seitz Karsten König Early evaluation of corneal collagen crosslinking in ex-vivo human corneas using two-photon imaging
description	Abstract The clinical outcome of corneal collagen crosslinking (CXL) is typically evaluated several weeks after treatment. An earlier assessment of its outcome could lead to an optimization of the treatment, including an immediate re-intervention in case of failure, thereby, avoiding additional discomfort and pain to the patient. In this study, we propose two-photon imaging (TPI) as an earlier evaluation method. CXL was performed in human corneas by application of riboflavin followed by UVA irradiation. Autofluorescence (AF) intensity and lifetime images were acquired using a commercial clinically certified multiphoton tomograph prior to CXL and after 2h, 24h, 72h, and 144h storage in culture medium. The first monitoring point was determined as the minimum time required for riboflavin clearance from the cornea. As control, untreated samples and samples treated only with riboflavin (without UVA irradiation) were monitored at the same time points. Significant increases in the stroma AF intensity and lifetime were observed as soon as 2h after treatment. A depth-dependent TPI analysis showed higher AF lifetimes anteriorly corresponding to areas were CXL was most effective. No alterations were observed in the control groups. Using TPI, the outcome of CXL can be assessed non-invasively and label-free much sooner than with conventional clinical devices.
format	article
author	Ana Batista Hans Georg Breunig Tobias Hager Berthold Seitz Karsten König
author_facet	Ana Batista Hans Georg Breunig Tobias Hager Berthold Seitz Karsten König
author_sort	Ana Batista
title	Early evaluation of corneal collagen crosslinking in ex-vivo human corneas using two-photon imaging
title_short	Early evaluation of corneal collagen crosslinking in ex-vivo human corneas using two-photon imaging
title_full	Early evaluation of corneal collagen crosslinking in ex-vivo human corneas using two-photon imaging
title_fullStr	Early evaluation of corneal collagen crosslinking in ex-vivo human corneas using two-photon imaging
title_full_unstemmed	Early evaluation of corneal collagen crosslinking in ex-vivo human corneas using two-photon imaging
title_sort	early evaluation of corneal collagen crosslinking in ex-vivo human corneas using two-photon imaging
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/a5c6042916684329aae24e8bedde2402
work_keys_str_mv	AT anabatista earlyevaluationofcornealcollagencrosslinkinginexvivohumancorneasusingtwophotonimaging AT hansgeorgbreunig earlyevaluationofcornealcollagencrosslinkinginexvivohumancorneasusingtwophotonimaging AT tobiashager earlyevaluationofcornealcollagencrosslinkinginexvivohumancorneasusingtwophotonimaging AT bertholdseitz earlyevaluationofcornealcollagencrosslinkinginexvivohumancorneasusingtwophotonimaging AT karstenkonig earlyevaluationofcornealcollagencrosslinkinginexvivohumancorneasusingtwophotonimaging
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Early evaluation of corneal collagen crosslinking in ex-vivo human corneas using two-photon imaging

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