Synergistic roles of eukaryotic translation elongation factors 1Bγ and 1A in stimulation of tombusvirus minus-strand synthesis.

Host factors are recruited into viral replicase complexes to aid replication of plus-strand RNA viruses. In this paper, we show that deletion of eukaryotic translation elongation factor 1Bgamma (eEF1Bγ) reduces Tomato bushy stunt virus (TBSV) replication in yeast host. Also, knock down of eEF1Bγ lev...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zsuzsanna Sasvari, Lara Izotova, Terri Goss Kinzy, Peter D Nagy
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a5c6ce80110546439bd4d9c1ab35e89e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a5c6ce80110546439bd4d9c1ab35e89e
record_format	dspace
spelling	oai:doaj.org-article:a5c6ce80110546439bd4d9c1ab35e89e2021-11-18T06:04:58ZSynergistic roles of eukaryotic translation elongation factors 1Bγ and 1A in stimulation of tombusvirus minus-strand synthesis.1553-73661553-737410.1371/journal.ppat.1002438https://doaj.org/article/a5c6ce80110546439bd4d9c1ab35e89e2011-12-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22194687/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Host factors are recruited into viral replicase complexes to aid replication of plus-strand RNA viruses. In this paper, we show that deletion of eukaryotic translation elongation factor 1Bgamma (eEF1Bγ) reduces Tomato bushy stunt virus (TBSV) replication in yeast host. Also, knock down of eEF1Bγ level in plant host decreases TBSV accumulation. eEF1Bγ binds to the viral RNA and is one of the resident host proteins in the tombusvirus replicase complex. Additional in vitro assays with whole cell extracts prepared from yeast strains lacking eEF1Bγ demonstrated its role in minus-strand synthesis by opening of the structured 3' end of the viral RNA and reducing the possibility of re-utilization of (+)-strand templates for repeated (-)-strand synthesis within the replicase. We also show that eEF1Bγ plays a synergistic role with eukaryotic translation elongation factor 1A in tombusvirus replication, possibly via stimulation of the proper positioning of the viral RNA-dependent RNA polymerase over the promoter region in the viral RNA template.These roles for translation factors during TBSV replication are separate from their canonical roles in host and viral protein translation.Zsuzsanna SasvariLara IzotovaTerri Goss KinzyPeter D NagyPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 7, Iss 12, p e1002438 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Zsuzsanna Sasvari Lara Izotova Terri Goss Kinzy Peter D Nagy Synergistic roles of eukaryotic translation elongation factors 1Bγ and 1A in stimulation of tombusvirus minus-strand synthesis.
description	Host factors are recruited into viral replicase complexes to aid replication of plus-strand RNA viruses. In this paper, we show that deletion of eukaryotic translation elongation factor 1Bgamma (eEF1Bγ) reduces Tomato bushy stunt virus (TBSV) replication in yeast host. Also, knock down of eEF1Bγ level in plant host decreases TBSV accumulation. eEF1Bγ binds to the viral RNA and is one of the resident host proteins in the tombusvirus replicase complex. Additional in vitro assays with whole cell extracts prepared from yeast strains lacking eEF1Bγ demonstrated its role in minus-strand synthesis by opening of the structured 3' end of the viral RNA and reducing the possibility of re-utilization of (+)-strand templates for repeated (-)-strand synthesis within the replicase. We also show that eEF1Bγ plays a synergistic role with eukaryotic translation elongation factor 1A in tombusvirus replication, possibly via stimulation of the proper positioning of the viral RNA-dependent RNA polymerase over the promoter region in the viral RNA template.These roles for translation factors during TBSV replication are separate from their canonical roles in host and viral protein translation.
format	article
author	Zsuzsanna Sasvari Lara Izotova Terri Goss Kinzy Peter D Nagy
author_facet	Zsuzsanna Sasvari Lara Izotova Terri Goss Kinzy Peter D Nagy
author_sort	Zsuzsanna Sasvari
title	Synergistic roles of eukaryotic translation elongation factors 1Bγ and 1A in stimulation of tombusvirus minus-strand synthesis.
title_short	Synergistic roles of eukaryotic translation elongation factors 1Bγ and 1A in stimulation of tombusvirus minus-strand synthesis.
title_full	Synergistic roles of eukaryotic translation elongation factors 1Bγ and 1A in stimulation of tombusvirus minus-strand synthesis.
title_fullStr	Synergistic roles of eukaryotic translation elongation factors 1Bγ and 1A in stimulation of tombusvirus minus-strand synthesis.
title_full_unstemmed	Synergistic roles of eukaryotic translation elongation factors 1Bγ and 1A in stimulation of tombusvirus minus-strand synthesis.
title_sort	synergistic roles of eukaryotic translation elongation factors 1bγ and 1a in stimulation of tombusvirus minus-strand synthesis.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/a5c6ce80110546439bd4d9c1ab35e89e
work_keys_str_mv	AT zsuzsannasasvari synergisticrolesofeukaryotictranslationelongationfactors1bgand1ainstimulationoftombusvirusminusstrandsynthesis AT laraizotova synergisticrolesofeukaryotictranslationelongationfactors1bgand1ainstimulationoftombusvirusminusstrandsynthesis AT terrigosskinzy synergisticrolesofeukaryotictranslationelongationfactors1bgand1ainstimulationoftombusvirusminusstrandsynthesis AT peterdnagy synergisticrolesofeukaryotictranslationelongationfactors1bgand1ainstimulationoftombusvirusminusstrandsynthesis
_version_	1718424624447881216

Synergistic roles of eukaryotic translation elongation factors 1Bγ and 1A in stimulation of tombusvirus minus-strand synthesis.

Ejemplares similares