3 ISOFORMS OF PML RARA IN ACUTE PROMYELOCYTIC LEUKAEMIA

Objective: To determine the frequencies of three isoforms of PML RARα fusion gene in APL. Study Design: Descriptive cross sectional study. Place and Duration of Study: Department of Hematology, Armed Forces Institute of Pathology (AFIP), Rawalpindi Pakistan, from Apr 2015 to Oct 2015. Ma...

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Autores principales:	Memoona Khan, Saleem Ahmed Khan, Chaudhry Altaf, Hamid Saeed Malik, Pervaz Ahmed, Haider Nisar
Formato:	article
Lenguaje:	EN
Publicado:	Army Medical College Rawalpindi 2018
Materias:	pml-rarα fusion gene isoforms Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a5cb5eb023204e8eb8073690000f563e
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spelling	oai:doaj.org-article:a5cb5eb023204e8eb8073690000f563e2021-11-26T03:48:14Z3 ISOFORMS OF PML RARA IN ACUTE PROMYELOCYTIC LEUKAEMIA0030-96482411-8842https://doaj.org/article/a5cb5eb023204e8eb8073690000f563e2018-12-01T00:00:00Zhttps://www.pafmj.org/index.php/PAFMJ/article/view/2575/2108https://doaj.org/toc/0030-9648https://doaj.org/toc/2411-8842Objective: To determine the frequencies of three isoforms of PML RARα fusion gene in APL. Study Design: Descriptive cross sectional study. Place and Duration of Study: Department of Hematology, Armed Forces Institute of Pathology (AFIP), Rawalpindi Pakistan, from Apr 2015 to Oct 2015. Material and Methods: This study involved 97 newly diagnosed cases of APL, aged between 15-70 years from both genders. Double nested PCR was carried out by Applied Biosystems 2720 Thermal Cycler in every patient to determine the isoform of PML RARα fusion gene. A written informed consent was obtained from every patient. Results: The age of the patients ranged from 17 years to 69 years with a mean of 37.87 ± 12.89 years. There were 68 (70.1%) male and 29 (29.9%) female patients in the study group. The bcr1 isoform was found in 9 (9.3%) patients. There was no significant difference in the frequency of bcr1 isoform across age groups; 17-34 years vs. 35-52 years vs. 53-69 years (14.6% vs. 7.0% vs. 0.0%; p=0.223) and genders; male vs. female (7.4% vs. 13.8%; p=0.317). The bcr2 isoform was found in 26 (26.8%) patients. There was no significant difference in the frequency of bcr2 isoform across age groups; 17-34 years vs. 35-52 years vs. 53-69 years (19.5% vs. 27.9% vs. 46.2%; p=0.164) and genders; male vs. female (25.0% vs. 31.0%; p=0.539) while the bcr3 isoform was found in 62 (63.9%) patients. There was no significant difference in the frequency of bcr3 isoform across age groups; 17-34 years vs. 35-52 years vs. 53-69 years (65.9% vs. 65.1% vs. 53.8%; p=0.717) and genders; male vs. female (67.6% vs. 55.2%; p=0.242). Conclusion: The most frequent isoform of PML-RARα fusion gene was bcr3 which was observed in 62 (63.9%) cases followed by bcr2 (26.8%) and bcr1 (9.3%). There was no significant difference in the frequency of isoforms with patient’s age and gender.Memoona KhanSaleem Ahmed KhanChaudhry AltafHamid Saeed MalikPervaz AhmedHaider NisarArmy Medical College Rawalpindiarticlepml-rarα fusion geneisoformsMedicineRMedicine (General)R5-920ENPakistan Armed Forces Medical Journal, Vol 68, Iss 6, Pp 1677-1682 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	pml-rarα fusion gene isoforms Medicine R Medicine (General) R5-920
spellingShingle	pml-rarα fusion gene isoforms Medicine R Medicine (General) R5-920 Memoona Khan Saleem Ahmed Khan Chaudhry Altaf Hamid Saeed Malik Pervaz Ahmed Haider Nisar 3 ISOFORMS OF PML RARA IN ACUTE PROMYELOCYTIC LEUKAEMIA
description	Objective: To determine the frequencies of three isoforms of PML RARα fusion gene in APL. Study Design: Descriptive cross sectional study. Place and Duration of Study: Department of Hematology, Armed Forces Institute of Pathology (AFIP), Rawalpindi Pakistan, from Apr 2015 to Oct 2015. Material and Methods: This study involved 97 newly diagnosed cases of APL, aged between 15-70 years from both genders. Double nested PCR was carried out by Applied Biosystems 2720 Thermal Cycler in every patient to determine the isoform of PML RARα fusion gene. A written informed consent was obtained from every patient. Results: The age of the patients ranged from 17 years to 69 years with a mean of 37.87 ± 12.89 years. There were 68 (70.1%) male and 29 (29.9%) female patients in the study group. The bcr1 isoform was found in 9 (9.3%) patients. There was no significant difference in the frequency of bcr1 isoform across age groups; 17-34 years vs. 35-52 years vs. 53-69 years (14.6% vs. 7.0% vs. 0.0%; p=0.223) and genders; male vs. female (7.4% vs. 13.8%; p=0.317). The bcr2 isoform was found in 26 (26.8%) patients. There was no significant difference in the frequency of bcr2 isoform across age groups; 17-34 years vs. 35-52 years vs. 53-69 years (19.5% vs. 27.9% vs. 46.2%; p=0.164) and genders; male vs. female (25.0% vs. 31.0%; p=0.539) while the bcr3 isoform was found in 62 (63.9%) patients. There was no significant difference in the frequency of bcr3 isoform across age groups; 17-34 years vs. 35-52 years vs. 53-69 years (65.9% vs. 65.1% vs. 53.8%; p=0.717) and genders; male vs. female (67.6% vs. 55.2%; p=0.242). Conclusion: The most frequent isoform of PML-RARα fusion gene was bcr3 which was observed in 62 (63.9%) cases followed by bcr2 (26.8%) and bcr1 (9.3%). There was no significant difference in the frequency of isoforms with patient’s age and gender.
format	article
author	Memoona Khan Saleem Ahmed Khan Chaudhry Altaf Hamid Saeed Malik Pervaz Ahmed Haider Nisar
author_facet	Memoona Khan Saleem Ahmed Khan Chaudhry Altaf Hamid Saeed Malik Pervaz Ahmed Haider Nisar
author_sort	Memoona Khan
title	3 ISOFORMS OF PML RARA IN ACUTE PROMYELOCYTIC LEUKAEMIA
title_short	3 ISOFORMS OF PML RARA IN ACUTE PROMYELOCYTIC LEUKAEMIA
title_full	3 ISOFORMS OF PML RARA IN ACUTE PROMYELOCYTIC LEUKAEMIA
title_fullStr	3 ISOFORMS OF PML RARA IN ACUTE PROMYELOCYTIC LEUKAEMIA
title_full_unstemmed	3 ISOFORMS OF PML RARA IN ACUTE PROMYELOCYTIC LEUKAEMIA
title_sort	3 isoforms of pml rara in acute promyelocytic leukaemia
publisher	Army Medical College Rawalpindi
publishDate	2018
url	https://doaj.org/article/a5cb5eb023204e8eb8073690000f563e
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3 ISOFORMS OF PML RARA IN ACUTE PROMYELOCYTIC LEUKAEMIA

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