Detection of Lung Cancer Cells in Solutions Using a Terahertz Chemical Microscope

Cancer genome analysis has recently attracted attention for personalized cancer treatment. In this treatment, evaluation of the ratio of cancer cells in a specimen tissue is essential for the precise analysis of the genome. Conventionally, the evaluation takes at least two days and depends on the sk...

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Autores principales:	Yuichi Yoshida, Xue Ding, Kohei Iwatsuki, Katsuya Taniizumi, Hirofumi Inoue, Jin Wang, Kenji Sakai, Toshihiko Kiwa
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	terahertz cancer genomic medicine cancer cells Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/a5dd39dbc1304e4bbf676857424e140b
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spelling	oai:doaj.org-article:a5dd39dbc1304e4bbf676857424e140b2021-11-25T18:58:04ZDetection of Lung Cancer Cells in Solutions Using a Terahertz Chemical Microscope10.3390/s212276311424-8220https://doaj.org/article/a5dd39dbc1304e4bbf676857424e140b2021-11-01T00:00:00Zhttps://www.mdpi.com/1424-8220/21/22/7631https://doaj.org/toc/1424-8220Cancer genome analysis has recently attracted attention for personalized cancer treatment. In this treatment, evaluation of the ratio of cancer cells in a specimen tissue is essential for the precise analysis of the genome. Conventionally, the evaluation takes at least two days and depends on the skill of the pathologist. In our group, a terahertz chemical microscope (TCM) was developed to easily and quickly measure the number of cancer cells in a solution. In this study, an antibody was immobilized on a sensing plate using an avidin-biotin reaction to immobilize it for high density and to improve antibody alignment. In addition, as the detected terahertz signals vary depending on the sensitivity of the sensing plate, the sensitivity was evaluated using pH measurement. The result of the cancer cell detection was corrected using the result of pH measurement. These results indicate that a TCM is expected to be an excellent candidate for liquid biopsies in cancer diagnosis.Yuichi YoshidaXue DingKohei IwatsukiKatsuya TaniizumiHirofumi InoueJin WangKenji SakaiToshihiko KiwaMDPI AGarticleterahertzcancer genomic medicinecancer cellsChemical technologyTP1-1185ENSensors, Vol 21, Iss 7631, p 7631 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	terahertz cancer genomic medicine cancer cells Chemical technology TP1-1185
spellingShingle	terahertz cancer genomic medicine cancer cells Chemical technology TP1-1185 Yuichi Yoshida Xue Ding Kohei Iwatsuki Katsuya Taniizumi Hirofumi Inoue Jin Wang Kenji Sakai Toshihiko Kiwa Detection of Lung Cancer Cells in Solutions Using a Terahertz Chemical Microscope
description	Cancer genome analysis has recently attracted attention for personalized cancer treatment. In this treatment, evaluation of the ratio of cancer cells in a specimen tissue is essential for the precise analysis of the genome. Conventionally, the evaluation takes at least two days and depends on the skill of the pathologist. In our group, a terahertz chemical microscope (TCM) was developed to easily and quickly measure the number of cancer cells in a solution. In this study, an antibody was immobilized on a sensing plate using an avidin-biotin reaction to immobilize it for high density and to improve antibody alignment. In addition, as the detected terahertz signals vary depending on the sensitivity of the sensing plate, the sensitivity was evaluated using pH measurement. The result of the cancer cell detection was corrected using the result of pH measurement. These results indicate that a TCM is expected to be an excellent candidate for liquid biopsies in cancer diagnosis.
format	article
author	Yuichi Yoshida Xue Ding Kohei Iwatsuki Katsuya Taniizumi Hirofumi Inoue Jin Wang Kenji Sakai Toshihiko Kiwa
author_facet	Yuichi Yoshida Xue Ding Kohei Iwatsuki Katsuya Taniizumi Hirofumi Inoue Jin Wang Kenji Sakai Toshihiko Kiwa
author_sort	Yuichi Yoshida
title	Detection of Lung Cancer Cells in Solutions Using a Terahertz Chemical Microscope
title_short	Detection of Lung Cancer Cells in Solutions Using a Terahertz Chemical Microscope
title_full	Detection of Lung Cancer Cells in Solutions Using a Terahertz Chemical Microscope
title_fullStr	Detection of Lung Cancer Cells in Solutions Using a Terahertz Chemical Microscope
title_full_unstemmed	Detection of Lung Cancer Cells in Solutions Using a Terahertz Chemical Microscope
title_sort	detection of lung cancer cells in solutions using a terahertz chemical microscope
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a5dd39dbc1304e4bbf676857424e140b
work_keys_str_mv	AT yuichiyoshida detectionoflungcancercellsinsolutionsusingaterahertzchemicalmicroscope AT xueding detectionoflungcancercellsinsolutionsusingaterahertzchemicalmicroscope AT koheiiwatsuki detectionoflungcancercellsinsolutionsusingaterahertzchemicalmicroscope AT katsuyataniizumi detectionoflungcancercellsinsolutionsusingaterahertzchemicalmicroscope AT hirofumiinoue detectionoflungcancercellsinsolutionsusingaterahertzchemicalmicroscope AT jinwang detectionoflungcancercellsinsolutionsusingaterahertzchemicalmicroscope AT kenjisakai detectionoflungcancercellsinsolutionsusingaterahertzchemicalmicroscope AT toshihikokiwa detectionoflungcancercellsinsolutionsusingaterahertzchemicalmicroscope
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Detection of Lung Cancer Cells in Solutions Using a Terahertz Chemical Microscope

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