Mtss1 promotes cell-cell junction assembly and stability through the small GTPase Rac1.

Cell-cell junctions are an integral part of epithelia and are often disrupted in cancer cells during epithelial-to-mesenchymal transition (EMT), which is a main driver of metastatic spread. We show here that Metastasis suppressor-1 (Mtss1; Missing in Metastasis, MIM), a member of the IMD-family of p...

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Autores principales:	John C Dawson, Susann Bruche, Heather J Spence, Vania M M Braga, Laura M Machesky
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a5e57de7038d49d480ef17bb33be33eb
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spelling	oai:doaj.org-article:a5e57de7038d49d480ef17bb33be33eb2021-11-18T07:24:18ZMtss1 promotes cell-cell junction assembly and stability through the small GTPase Rac1.1932-620310.1371/journal.pone.0031141https://doaj.org/article/a5e57de7038d49d480ef17bb33be33eb2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22479308/?tool=EBIhttps://doaj.org/toc/1932-6203Cell-cell junctions are an integral part of epithelia and are often disrupted in cancer cells during epithelial-to-mesenchymal transition (EMT), which is a main driver of metastatic spread. We show here that Metastasis suppressor-1 (Mtss1; Missing in Metastasis, MIM), a member of the IMD-family of proteins, inhibits cell-cell junction disassembly in wound healing or HGF-induced scatter assays by enhancing cell-cell junction strength. Mtss1 not only makes cells more resistant to cell-cell junction disassembly, but also accelerates the kinetics of adherens junction assembly. Mtss1 drives enhanced junction formation specifically by elevating Rac-GTP. Lastly, we show that Mtss1 depletion reduces recruitment of F-actin at cell-cell junctions. We thus propose that Mtss1 promotes Rac1 activation and actin recruitment driving junction maintenance. We suggest that the observed loss of Mtss1 in cancers may compromise junction stability and thus promote EMT and metastasis.John C DawsonSusann BrucheHeather J SpenceVania M M BragaLaura M MacheskyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 3, p e31141 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q John C Dawson Susann Bruche Heather J Spence Vania M M Braga Laura M Machesky Mtss1 promotes cell-cell junction assembly and stability through the small GTPase Rac1.
description	Cell-cell junctions are an integral part of epithelia and are often disrupted in cancer cells during epithelial-to-mesenchymal transition (EMT), which is a main driver of metastatic spread. We show here that Metastasis suppressor-1 (Mtss1; Missing in Metastasis, MIM), a member of the IMD-family of proteins, inhibits cell-cell junction disassembly in wound healing or HGF-induced scatter assays by enhancing cell-cell junction strength. Mtss1 not only makes cells more resistant to cell-cell junction disassembly, but also accelerates the kinetics of adherens junction assembly. Mtss1 drives enhanced junction formation specifically by elevating Rac-GTP. Lastly, we show that Mtss1 depletion reduces recruitment of F-actin at cell-cell junctions. We thus propose that Mtss1 promotes Rac1 activation and actin recruitment driving junction maintenance. We suggest that the observed loss of Mtss1 in cancers may compromise junction stability and thus promote EMT and metastasis.
format	article
author	John C Dawson Susann Bruche Heather J Spence Vania M M Braga Laura M Machesky
author_facet	John C Dawson Susann Bruche Heather J Spence Vania M M Braga Laura M Machesky
author_sort	John C Dawson
title	Mtss1 promotes cell-cell junction assembly and stability through the small GTPase Rac1.
title_short	Mtss1 promotes cell-cell junction assembly and stability through the small GTPase Rac1.
title_full	Mtss1 promotes cell-cell junction assembly and stability through the small GTPase Rac1.
title_fullStr	Mtss1 promotes cell-cell junction assembly and stability through the small GTPase Rac1.
title_full_unstemmed	Mtss1 promotes cell-cell junction assembly and stability through the small GTPase Rac1.
title_sort	mtss1 promotes cell-cell junction assembly and stability through the small gtpase rac1.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/a5e57de7038d49d480ef17bb33be33eb
work_keys_str_mv	AT johncdawson mtss1promotescellcelljunctionassemblyandstabilitythroughthesmallgtpaserac1 AT susannbruche mtss1promotescellcelljunctionassemblyandstabilitythroughthesmallgtpaserac1 AT heatherjspence mtss1promotescellcelljunctionassemblyandstabilitythroughthesmallgtpaserac1 AT vaniammbraga mtss1promotescellcelljunctionassemblyandstabilitythroughthesmallgtpaserac1 AT laurammachesky mtss1promotescellcelljunctionassemblyandstabilitythroughthesmallgtpaserac1
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Mtss1 promotes cell-cell junction assembly and stability through the small GTPase Rac1.

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