Combination of RGD compound and low-dose paclitaxel induces apoptosis in human glioblastoma cells.

<h4>Background</h4>Integrins are a family of transmembrane adhesion proteins that mediate cell adhesion and intracellular signaling. Integrin-αvβ3 is expressed on the surface of human glioblastoma cells, and can be further induced by chemical stress. The Arg-Gly-Asp (RGD) motif-containin...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ming-Wei Chang, Jem-Mau Lo, Hsueh-Fen Juan, Hsin-Yi Chang, Chun-Yu Chuang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a5e96247eb21405582b5834a6395a8cc
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a5e96247eb21405582b5834a6395a8cc
record_format	dspace
spelling	oai:doaj.org-article:a5e96247eb21405582b5834a6395a8cc2021-11-18T07:17:29ZCombination of RGD compound and low-dose paclitaxel induces apoptosis in human glioblastoma cells.1932-620310.1371/journal.pone.0037935https://doaj.org/article/a5e96247eb21405582b5834a6395a8cc2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22655084/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Integrins are a family of transmembrane adhesion proteins that mediate cell adhesion and intracellular signaling. Integrin-αvβ3 is expressed on the surface of human glioblastoma cells, and can be further induced by chemical stress. The Arg-Gly-Asp (RGD) motif-containing peptides are specifically bound to integrin-αvβ3, and to inhibit neovasculature underlying competition to normal extracellular matrix proteins. This study employed two types of RGD peptides, cyclic RGD (c(RGDyK)) and bi-cyclic RGD (E[c(RGDyK)](2)) peptide, to human glioblastoma U87MG cells with combination of low dose Paclitaxel (PTX) pre-treatment to augment therapeutic activity for RGD peptide-induced apoptosis.<h4>Principal findings</h4>Human glioblastoma U87MG cells were treated with RGD peptides in the absence or presence of initial exposure to low-dose 10 nM PTX. Results showed that integrin-αvβ3 expressing on the surface of U87MG cells was induced by 10 nM PTX pre-treatment for 12 hrs. Additionally, the U87MG cells pre-treated with PTX and followed by RGD peptides exhibited greater expression of caspases-3, -8 and -9 than those merely treated with single agent of PTX or RGD peptide. Furthermore, the caspase-3, -8 and -9 inhibitor presented significant protection against E[c(RGDyK)](2) peptide induced U87MG programmed cell death. The increased expression of PTX-induced integrin-αvβ3 was correlated with the enhanced apoptosis in U87MG cells.<h4>Conclusions</h4>This study provides a novel concept of targeting integrin-αvβ3 with RGD peptides in combination with low-dose PTX pre-treatment to improve efficiency in human glioblastoma treatment.Ming-Wei ChangJem-Mau LoHsueh-Fen JuanHsin-Yi ChangChun-Yu ChuangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 5, p e37935 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ming-Wei Chang Jem-Mau Lo Hsueh-Fen Juan Hsin-Yi Chang Chun-Yu Chuang Combination of RGD compound and low-dose paclitaxel induces apoptosis in human glioblastoma cells.
description	<h4>Background</h4>Integrins are a family of transmembrane adhesion proteins that mediate cell adhesion and intracellular signaling. Integrin-αvβ3 is expressed on the surface of human glioblastoma cells, and can be further induced by chemical stress. The Arg-Gly-Asp (RGD) motif-containing peptides are specifically bound to integrin-αvβ3, and to inhibit neovasculature underlying competition to normal extracellular matrix proteins. This study employed two types of RGD peptides, cyclic RGD (c(RGDyK)) and bi-cyclic RGD (E[c(RGDyK)](2)) peptide, to human glioblastoma U87MG cells with combination of low dose Paclitaxel (PTX) pre-treatment to augment therapeutic activity for RGD peptide-induced apoptosis.<h4>Principal findings</h4>Human glioblastoma U87MG cells were treated with RGD peptides in the absence or presence of initial exposure to low-dose 10 nM PTX. Results showed that integrin-αvβ3 expressing on the surface of U87MG cells was induced by 10 nM PTX pre-treatment for 12 hrs. Additionally, the U87MG cells pre-treated with PTX and followed by RGD peptides exhibited greater expression of caspases-3, -8 and -9 than those merely treated with single agent of PTX or RGD peptide. Furthermore, the caspase-3, -8 and -9 inhibitor presented significant protection against E[c(RGDyK)](2) peptide induced U87MG programmed cell death. The increased expression of PTX-induced integrin-αvβ3 was correlated with the enhanced apoptosis in U87MG cells.<h4>Conclusions</h4>This study provides a novel concept of targeting integrin-αvβ3 with RGD peptides in combination with low-dose PTX pre-treatment to improve efficiency in human glioblastoma treatment.
format	article
author	Ming-Wei Chang Jem-Mau Lo Hsueh-Fen Juan Hsin-Yi Chang Chun-Yu Chuang
author_facet	Ming-Wei Chang Jem-Mau Lo Hsueh-Fen Juan Hsin-Yi Chang Chun-Yu Chuang
author_sort	Ming-Wei Chang
title	Combination of RGD compound and low-dose paclitaxel induces apoptosis in human glioblastoma cells.
title_short	Combination of RGD compound and low-dose paclitaxel induces apoptosis in human glioblastoma cells.
title_full	Combination of RGD compound and low-dose paclitaxel induces apoptosis in human glioblastoma cells.
title_fullStr	Combination of RGD compound and low-dose paclitaxel induces apoptosis in human glioblastoma cells.
title_full_unstemmed	Combination of RGD compound and low-dose paclitaxel induces apoptosis in human glioblastoma cells.
title_sort	combination of rgd compound and low-dose paclitaxel induces apoptosis in human glioblastoma cells.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/a5e96247eb21405582b5834a6395a8cc
work_keys_str_mv	AT mingweichang combinationofrgdcompoundandlowdosepaclitaxelinducesapoptosisinhumanglioblastomacells AT jemmaulo combinationofrgdcompoundandlowdosepaclitaxelinducesapoptosisinhumanglioblastomacells AT hsuehfenjuan combinationofrgdcompoundandlowdosepaclitaxelinducesapoptosisinhumanglioblastomacells AT hsinyichang combinationofrgdcompoundandlowdosepaclitaxelinducesapoptosisinhumanglioblastomacells AT chunyuchuang combinationofrgdcompoundandlowdosepaclitaxelinducesapoptosisinhumanglioblastomacells
_version_	1718423683679125504

Combination of RGD compound and low-dose paclitaxel induces apoptosis in human glioblastoma cells.

Ejemplares similares