The study on the function and cell source of interleukin‐6 in interstitial cystitis/bladder painful syndrome rat model

Abstract Objective The elevated expression of interleukin‐6 (IL‐6) in patients with interstitial cystitis/bladder painful syndrome (IC/BPS) has been demonstrated, but the role of IL‐6 in IC/BPS and its source remain to be explored. Methods IC/BPS rat model was created in female rats by using long‐te...

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Autores principales:	Zhenming Zheng, Jiapeng Zhang, Caixia Zhang, Wenshuang Li, Kaiqun Ma, Hao Huang, Kuiqing Li, Yousheng Yao
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	cell source interleukin‐6 interstitial cystitis/bladder painful syndrome macrophages Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/a5e97d83879a48ba89768860a1b78c99
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spelling	oai:doaj.org-article:a5e97d83879a48ba89768860a1b78c992021-11-12T19:57:15ZThe study on the function and cell source of interleukin‐6 in interstitial cystitis/bladder painful syndrome rat model2050-452710.1002/iid3.505https://doaj.org/article/a5e97d83879a48ba89768860a1b78c992021-12-01T00:00:00Zhttps://doi.org/10.1002/iid3.505https://doaj.org/toc/2050-4527Abstract Objective The elevated expression of interleukin‐6 (IL‐6) in patients with interstitial cystitis/bladder painful syndrome (IC/BPS) has been demonstrated, but the role of IL‐6 in IC/BPS and its source remain to be explored. Methods IC/BPS rat model was created in female rats by using long‐term intermittent intravesical hyaluronidase (0.5 ml, 4 mg/ml). After modeling, IL‐6 stimulation group, and anti‐IL‐6R group were treated with recombinant rat IL‐6 and tocilizumab, respectively. Symptomatic changes were detected by Vonfrey pain score and urodynamics, and hematoxylin‐eosin (HE) staining, mast cell staining and Masson staining were used to evaluate the changes of inflammation in the bladder tissue of rats. Cell sources of IL‐6 was explored through enzyme linked immunosorbent assay (ELISA) test, reverse transcription polymerase chain reaction (RT‐PCR), and western‐blot test on the supernatant of coculturing rat bladder epithelial cells and rat macrophages. Results The Vonfrey pain scores of the model group and IL‐6 stimulation group were significantly higher than those of the control group, while the anti‐IL‐6R group were significantly lower (p < .05). Compared with the blank control group, urodynamic results showed that the urination interval of the model group and IL‐6 stimulation group was significantly shortened, and the maximum bladder capacity was significantly reduced (p < .05), and anti‐IL‐6R treatment significantly alleviated the inflammatory response of bladder tissue. The results of HE, Mast cell staining, and Masson staining showed that the inflammatory response of bladder tissue after anti‐IL‐6R treatment was significantly reduced. Through cells coculture, the relative expression of IL‐6 from model group was found significantly higher than blank control group by RT‐PCR, ELISA, and western blot test (p < .05). Conclusions IL‐6 played an essential role in the development of IC/BPS rat model as a proinflammation cytokine. Further evidence from coculture proved that macrophages are the cell resource of IL‐6 in IC/BPS.Zhenming ZhengJiapeng ZhangCaixia ZhangWenshuang LiKaiqun MaHao HuangKuiqing LiYousheng YaoWileyarticlecell sourceinterleukin‐6interstitial cystitis/bladder painful syndromemacrophagesImmunologic diseases. AllergyRC581-607ENImmunity, Inflammation and Disease, Vol 9, Iss 4, Pp 1520-1528 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cell source interleukin‐6 interstitial cystitis/bladder painful syndrome macrophages Immunologic diseases. Allergy RC581-607
spellingShingle	cell source interleukin‐6 interstitial cystitis/bladder painful syndrome macrophages Immunologic diseases. Allergy RC581-607 Zhenming Zheng Jiapeng Zhang Caixia Zhang Wenshuang Li Kaiqun Ma Hao Huang Kuiqing Li Yousheng Yao The study on the function and cell source of interleukin‐6 in interstitial cystitis/bladder painful syndrome rat model
description	Abstract Objective The elevated expression of interleukin‐6 (IL‐6) in patients with interstitial cystitis/bladder painful syndrome (IC/BPS) has been demonstrated, but the role of IL‐6 in IC/BPS and its source remain to be explored. Methods IC/BPS rat model was created in female rats by using long‐term intermittent intravesical hyaluronidase (0.5 ml, 4 mg/ml). After modeling, IL‐6 stimulation group, and anti‐IL‐6R group were treated with recombinant rat IL‐6 and tocilizumab, respectively. Symptomatic changes were detected by Vonfrey pain score and urodynamics, and hematoxylin‐eosin (HE) staining, mast cell staining and Masson staining were used to evaluate the changes of inflammation in the bladder tissue of rats. Cell sources of IL‐6 was explored through enzyme linked immunosorbent assay (ELISA) test, reverse transcription polymerase chain reaction (RT‐PCR), and western‐blot test on the supernatant of coculturing rat bladder epithelial cells and rat macrophages. Results The Vonfrey pain scores of the model group and IL‐6 stimulation group were significantly higher than those of the control group, while the anti‐IL‐6R group were significantly lower (p < .05). Compared with the blank control group, urodynamic results showed that the urination interval of the model group and IL‐6 stimulation group was significantly shortened, and the maximum bladder capacity was significantly reduced (p < .05), and anti‐IL‐6R treatment significantly alleviated the inflammatory response of bladder tissue. The results of HE, Mast cell staining, and Masson staining showed that the inflammatory response of bladder tissue after anti‐IL‐6R treatment was significantly reduced. Through cells coculture, the relative expression of IL‐6 from model group was found significantly higher than blank control group by RT‐PCR, ELISA, and western blot test (p < .05). Conclusions IL‐6 played an essential role in the development of IC/BPS rat model as a proinflammation cytokine. Further evidence from coculture proved that macrophages are the cell resource of IL‐6 in IC/BPS.
format	article
author	Zhenming Zheng Jiapeng Zhang Caixia Zhang Wenshuang Li Kaiqun Ma Hao Huang Kuiqing Li Yousheng Yao
author_facet	Zhenming Zheng Jiapeng Zhang Caixia Zhang Wenshuang Li Kaiqun Ma Hao Huang Kuiqing Li Yousheng Yao
author_sort	Zhenming Zheng
title	The study on the function and cell source of interleukin‐6 in interstitial cystitis/bladder painful syndrome rat model
title_short	The study on the function and cell source of interleukin‐6 in interstitial cystitis/bladder painful syndrome rat model
title_full	The study on the function and cell source of interleukin‐6 in interstitial cystitis/bladder painful syndrome rat model
title_fullStr	The study on the function and cell source of interleukin‐6 in interstitial cystitis/bladder painful syndrome rat model
title_full_unstemmed	The study on the function and cell source of interleukin‐6 in interstitial cystitis/bladder painful syndrome rat model
title_sort	study on the function and cell source of interleukin‐6 in interstitial cystitis/bladder painful syndrome rat model
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/a5e97d83879a48ba89768860a1b78c99
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The study on the function and cell source of interleukin‐6 in interstitial cystitis/bladder painful syndrome rat model

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